##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527798_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	899386
Sequences flagged as poor quality	0
Sequence length	50
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.505567131354056	33.0	31.0	34.0	31.0	34.0
2	33.062663861790156	34.0	31.0	34.0	31.0	34.0
3	33.232628704471715	34.0	34.0	34.0	31.0	34.0
4	36.30962901357148	37.0	37.0	37.0	35.0	37.0
5	36.41711901230395	37.0	37.0	37.0	35.0	37.0
6	35.59711959047617	37.0	35.0	37.0	35.0	37.0
7	36.387440987518154	37.0	37.0	37.0	35.0	37.0
8	36.66828147202647	37.0	37.0	37.0	35.0	37.0
9	38.7626903242879	39.0	39.0	39.0	39.0	39.0
10	38.285140084457616	39.0	39.0	39.0	37.0	39.0
11	37.994845372287315	39.0	38.0	39.0	35.0	39.0
12	37.295677273161914	39.0	37.0	39.0	35.0	39.0
13	37.206635415716946	39.0	37.0	39.0	35.0	39.0
14	37.953926345306684	40.0	37.0	40.0	34.0	40.0
15	37.97241784951066	40.0	37.0	40.0	34.0	40.0
16	37.950887605544224	40.0	37.0	40.0	34.0	40.0
17	37.73069516314463	40.0	37.0	40.0	33.0	40.0
18	37.6311083339078	40.0	36.0	40.0	33.0	40.0
19	37.56879137544947	40.0	36.0	40.0	33.0	40.0
20	37.46025733111256	40.0	36.0	40.0	33.0	40.0
21	37.44315121649659	40.0	36.0	40.0	33.0	40.0
22	37.5231546855299	40.0	36.0	40.0	33.0	40.0
23	37.447747685643314	40.0	35.0	40.0	33.0	40.0
24	37.37707391487081	40.0	35.0	40.0	33.0	40.0
25	37.360400317549974	39.0	35.0	40.0	33.0	40.0
26	37.243386043367366	39.0	35.0	40.0	33.0	40.0
27	37.102038501822356	39.0	35.0	40.0	33.0	40.0
28	36.89375974275784	39.0	35.0	40.0	32.0	40.0
29	36.982286804553326	39.0	35.0	40.0	33.0	40.0
30	37.088096768239666	39.0	35.0	40.0	33.0	40.0
31	37.11550657893274	39.0	35.0	40.0	33.0	40.0
32	37.030047165510695	39.0	35.0	40.0	33.0	40.0
33	36.87745417429224	39.0	35.0	40.0	33.0	40.0
34	36.39773022928976	39.0	35.0	40.0	31.0	40.0
35	36.37190260911333	39.0	35.0	40.0	31.0	40.0
36	36.334865119092356	38.0	35.0	40.0	31.0	40.0
37	36.2901090299382	38.0	35.0	40.0	32.0	40.0
38	36.215791662311844	37.0	35.0	40.0	32.0	40.0
39	36.08264860693851	37.0	35.0	40.0	32.0	40.0
40	35.798108932093676	37.0	35.0	40.0	31.0	40.0
41	35.69619495967249	36.0	35.0	40.0	31.0	40.0
42	35.52716186376039	36.0	35.0	40.0	31.0	40.0
43	35.41319855990643	36.0	35.0	40.0	31.0	40.0
44	35.07976664079717	35.0	34.0	39.0	30.0	40.0
45	35.015919749695904	35.0	34.0	39.0	30.0	40.0
46	34.842348001859044	35.0	34.0	39.0	30.0	40.0
47	34.71513343547709	35.0	34.0	39.0	30.0	40.0
48	34.58166793790431	35.0	34.0	39.0	29.0	40.0
49	34.45824818264905	35.0	34.0	38.0	29.0	40.0
50	33.94250855583698	35.0	33.0	37.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	9.0
16	26.0
17	72.0
18	148.0
19	235.0
20	495.0
21	819.0
22	1235.0
23	1774.0
24	2506.0
25	3510.0
26	4788.0
27	6073.0
28	8044.0
29	9963.0
30	13114.0
31	16904.0
32	22616.0
33	34472.0
34	82926.0
35	89323.0
36	104839.0
37	165019.0
38	237298.0
39	93175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.40981958803006	0.4864429733173521	0.08650345902649141	0.017233979626100474
8	99.22280311234553	0.558158565954996	0.1150785091162193	0.10395981258325125
9	98.65019024089769	0.7912064452860061	0.2589544422528258	0.2996488715634889
10	67.63458626218332	24.32670733144612	2.8587280655914147	5.1799783407791535
11	41.63596053307479	23.419532881321256	18.148603602902426	16.795902982701534
12	34.96874534404583	15.956107833566454	24.226083127822758	24.84906369456496
13	27.484194772878386	16.07997011294372	27.297289484159194	29.138545630018704
14	27.638077532894663	16.57419617383415	29.516025377312964	26.271700915958217
15	29.144327352215843	17.18027632184624	27.037667920114387	26.63772840582353
16	34.567249212240355	17.950690804615594	23.636013902818146	23.846046080325912
17	34.9765284316189	18.718881548078357	22.344577300513908	23.960012719788836
18	32.87398291723464	20.076363207788425	22.95343712265924	24.09621675231769
19	33.04298710453576	22.121647435027896	20.40836748626285	24.426997974173492
20	33.165959888190386	22.022357474988492	20.19366545621124	24.618017180609883
21	33.01563511106466	21.63264716150796	22.379378820662097	22.972338906765284
22	32.011616814137646	19.147062551562954	25.618588681611676	23.222731952687724
23	32.89232876651405	20.215680475346513	22.716275325611026	24.175715432528413
24	33.45793685914613	19.12760483263026	22.456209013704907	24.958249294518705
25	31.11178070372454	19.888679610311925	21.175557547037645	27.82398213892589
26	27.85911721997007	19.326184752709068	23.83170296179838	28.982995065522477
27	26.07601185697798	18.480163133515532	23.566966797348414	31.876858212158073
28	28.452744427865234	22.607089725657282	20.972863709241636	27.96730213723585
29	31.422548271821	21.112069789834397	21.50811776033872	25.957264178005886
30	29.657455197212318	20.23413751159124	23.744532380979912	26.363874910216527
31	34.48041219231787	20.160309366612335	20.47007625202082	24.889202189048973
32	35.65399061137265	19.845316693833347	21.189567104669184	23.311125590124817
33	35.43606415932648	19.123046167051744	22.505798400241943	22.93509127337984
34	31.270333316284667	21.454748016980474	26.274814150987453	21.00010451574741
35	28.85412937270538	24.986490783712444	25.205084357550593	20.954295486031583
36	28.723039940581685	26.13327314412277	22.255182980388845	22.888503934906705
37	31.716526608152673	26.312951280095533	19.619940715110086	22.350581396641708
38	32.38742875695196	25.739004165063722	20.189996286355356	21.683570791628956
39	33.678642985325546	22.359587540833413	19.595590769702888	24.366178704138157
40	28.902384515658458	23.34592711027301	20.428825887883512	27.322862486185016
41	27.49264498224344	23.762989417224638	21.210915002012484	27.533450598519433
42	25.183736460207296	22.06394140002179	21.465088404756134	31.287233735014773
43	26.30005359211729	19.813406034783732	24.23953675062765	29.64700362247133
44	26.96906556250598	19.509420871572384	23.1087653132248	30.412748252696844
45	28.975434351880057	21.386479220268047	22.314445632909564	27.323640794942328
46	28.53735770848112	24.1711567669499	21.197461379207596	26.094024145361388
47	26.489738554969723	21.69257693582066	23.988143022017244	27.829541487192373
48	27.925273464341227	21.141867896542752	25.47193307434183	25.46092556477419
49	29.544489240437365	20.13962859106101	23.96379307661004	26.352089091891578
50	26.557784977751485	20.774506163093488	24.84528333774375	27.822425521411276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	24.0
10	48.0
11	35.0
12	22.0
13	19.5
14	17.0
15	138.0
16	259.0
17	503.5
18	748.0
19	906.0
20	1064.0
21	969.0
22	874.0
23	788.5
24	703.0
25	925.5
26	1148.0
27	1253.0
28	1358.0
29	1656.5
30	1955.0
31	2380.5
32	2806.0
33	3424.5
34	4043.0
35	4101.5
36	4160.0
37	4802.0
38	5444.0
39	7294.5
40	9145.0
41	9388.5
42	9632.0
43	11587.5
44	13543.0
45	15476.5
46	17410.0
47	18837.5
48	20265.0
49	21325.0
50	22385.0
51	24427.0
52	26469.0
53	30112.0
54	33755.0
55	36378.0
56	39001.0
57	43095.5
58	47190.0
59	51883.5
60	56577.0
61	67006.5
62	77436.0
63	81137.5
64	84839.0
65	91199.5
66	97560.0
67	103751.5
68	109943.0
69	87688.0
70	65433.0
71	56661.5
72	47890.0
73	39668.5
74	31447.0
75	27668.0
76	23889.0
77	19541.5
78	15194.0
79	16014.0
80	16834.0
81	11484.5
82	6135.0
83	3912.0
84	1689.0
85	1179.0
86	669.0
87	448.5
88	228.0
89	158.5
90	89.0
91	74.0
92	59.0
93	41.0
94	23.0
95	13.5
96	4.0
97	4.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	899386.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.266655251471558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.42446276231956	6.340659071855688
2	9.016954033332134	1.6711400889050976
3	3.486795531718321	0.9693279637441543
4	1.6330105707737903	0.6053018392547804
5	0.905894916189722	0.4197307941195437
6	0.6179283203148435	0.3435677228687126
7	0.40555295585712053	0.263068359970024
8	0.3635578272920341	0.26951720395914547
9	0.32156269872694765	0.2681829603751893
>10	8.841774354174916	24.29913296404436
>50	4.242707845889877	26.94760647819735
>100	1.6366101532222264	25.518520412814965
>500	0.07559123141715561	4.9040122928308865
>1k	0.022797355506761215	3.9788255543226154
>5k	0.0035995824484359815	1.883951940546106
>10k+	0.0011998608161453272	1.317454352191384
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	11849	1.317454352191384	No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5815	0.646552203392092	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5788	0.6435501553281905	No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	5341	0.5938495818258234	No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4774	0.5308065724838945	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	3241	0.3603569546334944	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	3206	0.3564654108469556	No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2367	0.26317954693535367	No Hit
GGGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGC	2301	0.25584120722359477	No Hit
GGGCGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2225	0.24739099785853905	No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2018	0.2243752960352952	No Hit
GGGCGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1715	0.190685645540402	No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1559	0.17334047894897187	No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1521	0.169115374266444	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGTCTG	1346	0.14965765533374992	No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1322	0.1469891681658376	No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1285	0.1428752504486394	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGC	1283	0.14265287651798003	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTG	1188	0.13209011481166041	No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1169	0.12997756247039646	No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1127	0.12530770992654988	No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1123	0.12486296206523116	No Hit
GGGCGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	1015	0.11285476980962567	No Hit
GGGCGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	991	0.11018628264171335	No Hit
GGGCGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	990	0.11007509567638367	No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	989	0.10996390871105398	No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	985	0.10951916084973527	No Hit
GGGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGTGCCGACTG	958	0.1065171127858339	No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	942	0.10473812134055902	No Hit
GGGCGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	929	0.10329269079127315	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	921	0.10240319506863572	No Hit
GGGCGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	903	0.10040182969270146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.10095776451934987	0.0	0.0	0.0	0.0
7	0.10206963417264667	0.0	0.0	0.0	0.0
8	0.10462693437522932	0.0	0.0	0.0	0.0
9	0.11763580931880194	0.0	0.0	0.0	0.0
10	0.1439871201019362	0.0	0.0	0.0	0.0
11	0.1862381669272148	0.0	0.0	0.0	0.0
12	0.19491075022292986	0.0	0.0	0.0	0.0
13	0.20869793392381025	0.0	0.0	0.0	0.0
14	0.22570953961925136	0.0	0.0	0.0	0.0
15	0.24238758441870342	0.0	0.0	0.0	0.0
16	0.254729337570298	0.0	0.0	0.0	0.0
17	0.2664039689299144	0.0	0.0	0.0	0.0
18	0.2803023395961245	0.0	0.0	0.0	0.0
19	0.3013166760434341	0.0	0.0	0.0	0.0
20	0.3172164120855784	0.0	0.0	0.0	0.0
21	0.3304476609598104	0.0	0.0	0.0	0.0
22	0.34000973997816286	0.0	0.0	0.0	0.0
23	0.3563542238816259	0.0	0.0	0.0	0.0
24	0.3709197163398141	0.0	0.0	0.0	0.0
25	0.3848180870060241	0.0	0.0	0.0	0.0
26	0.3992723924988826	0.0	0.0	0.0	0.0
27	0.41361551102641136	0.0	0.0	0.0	0.0
28	0.4320725472711383	0.0	0.0	0.0	0.0
29	0.4440807395267438	0.0	0.0	0.0	0.0
30	0.45931335377691	0.0	0.0	0.0	0.0
31	0.4729893505124607	0.0	0.0	0.0	0.0
32	0.488555525658616	0.0	0.0	0.0	0.0
33	0.5018979614981777	0.0	0.0	0.0	0.0
34	0.5165746409216955	0.0	0.0	0.0	0.0
35	0.5332526857211476	0.0	0.0	0.0	0.0
36	0.550820226243237	0.0	0.0	0.0	0.0
37	0.5686101406959859	0.0	0.0	0.0	0.0
38	0.5818413895702179	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTATC	30	2.5271002E-6	44.000004	14
ATACGGA	60	0.0	44.000004	10
TACGTAT	30	2.5271002E-6	44.000004	13
TCACGCG	25	4.442065E-5	44.0	39
AACGTTA	35	1.4456054E-7	44.0	37
TCCGATT	25	4.442065E-5	44.0	24
TCCGATA	20	7.8555674E-4	44.0	26
ACGTTAT	35	1.4456054E-7	44.0	38
CCGATAG	20	7.8555674E-4	44.0	27
TTATGCG	25	4.442065E-5	44.0	24
GCAAACG	20	7.8555674E-4	44.0	43
CGACAAA	25	4.442065E-5	44.0	31
TTATAAG	25	4.442065E-5	44.0	20
CCGTATC	20	7.8555674E-4	44.0	44
ACGATAA	20	7.8555674E-4	44.0	32
GTTCGTT	35	1.4456054E-7	44.0	26
GTTCGAA	25	4.442065E-5	44.0	29
TACGATA	20	7.8555674E-4	44.0	31
TCGTAAG	20	7.8555674E-4	44.0	18
AGTTATG	20	7.8555674E-4	44.0	38
>>END_MODULE