FastQCFastQC Report
Fri 17 Jun 2016
SRR1527797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352100
Sequences flagged as poor quality0
Sequence length52
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC178431.3196509133939798No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG87560.6475852377782708No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT87560.6475852377782708No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC77130.5704459729309963No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT72600.5369425338362547No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC49640.3671326085348717No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC47090.3482730567265735No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC36570.270468160639006No Hit
GGGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC35120.25974410176762075No Hit
GGGCGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT33610.24857628873604024No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC28620.21167073441313514No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG26850.1985799866873752No Hit
GGGCGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25390.18778196878929074No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGTCTGCT20430.15109829154648324No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT20170.14917535685230382No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC19750.14606907773093708No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC18840.13933880630130907No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT18620.1377117077139265No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT18530.13704607647363362No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17230.12743140300273648No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG17220.1273574439760373No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT16390.12121884476000297No Hit
GGGCGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG16390.12121884476000297No Hit
GGGCGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA15140.11197396642260188No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC14950.11056874491531693No Hit
GGGCGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT14760.10916352340803194No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG14660.10842393314103986No Hit
GGGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGTGCCGACTGCT14270.10553953109977071No Hit
GGGCGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT14240.1053176540196731No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT13830.10228533392500555No Hit
GGGCGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT13620.10073219436432218No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT13590.10051031728422453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA206.311852E-446.037
AGCGTAC206.311852E-446.011
CATATCG253.417478E-546.022
GGTCGAC301.8616483E-646.09
ACGTTAT206.311852E-446.038
CGCATTC206.311852E-446.045
TAGCGTC206.311852E-446.022
GACGTAT206.311852E-446.010
TAACGCA206.311852E-446.027
ATCACGC206.311852E-446.043
ATATCGT301.8616483E-646.023
TACGATA301.8616483E-646.031
TAACACG206.311852E-446.042
TATTCGC206.311852E-446.012
TCGTATT551.8189894E-1246.022
ACGTATC253.417478E-546.014
ATAACCG206.311852E-446.011
AACTCGT206.311852E-446.020
TCATACG253.417478E-546.041
ATACGTA253.417478E-546.043
Produced by FastQC (version 0.11.5)