##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1352100
Sequences flagged as poor quality	0
Sequence length	52
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14825086901856	33.0	31.0	34.0	31.0	34.0
2	32.74926262850381	34.0	31.0	34.0	31.0	34.0
3	32.933784483396195	34.0	31.0	34.0	31.0	34.0
4	36.11911249167961	37.0	37.0	37.0	35.0	37.0
5	36.19001701057614	37.0	37.0	37.0	35.0	37.0
6	35.300641224761485	37.0	35.0	37.0	32.0	37.0
7	36.29975001848975	37.0	35.0	37.0	35.0	37.0
8	36.5909925301383	37.0	37.0	37.0	35.0	37.0
9	38.686431476961765	39.0	39.0	39.0	38.0	39.0
10	38.05000295836107	39.0	38.0	39.0	37.0	39.0
11	37.66119887582279	39.0	38.0	39.0	35.0	39.0
12	36.94778714592116	39.0	35.0	39.0	34.0	39.0
13	36.83537904001183	39.0	35.0	39.0	33.0	39.0
14	37.61517121514681	40.0	36.0	40.0	33.0	40.0
15	37.66830264033725	40.0	36.0	40.0	33.0	40.0
16	37.65743805931514	40.0	36.0	40.0	33.0	40.0
17	37.47170919310702	40.0	36.0	40.0	33.0	40.0
18	37.44030101323867	40.0	36.0	40.0	33.0	40.0
19	37.41585015901191	40.0	36.0	40.0	33.0	40.0
20	37.37591376377487	40.0	35.0	40.0	33.0	40.0
21	37.35515494416094	40.0	35.0	40.0	33.0	40.0
22	37.351886694771096	40.0	35.0	40.0	33.0	40.0
23	37.31608978625841	40.0	35.0	40.0	33.0	40.0
24	37.22344870941498	39.0	35.0	40.0	33.0	40.0
25	37.2168944604689	39.0	35.0	40.0	33.0	40.0
26	37.07135049182753	39.0	35.0	40.0	33.0	40.0
27	36.95100066563124	39.0	35.0	40.0	32.0	40.0
28	36.804368759707124	39.0	35.0	40.0	32.0	40.0
29	36.69055839065158	39.0	35.0	40.0	32.0	40.0
30	36.48671991716589	38.0	35.0	40.0	31.0	40.0
31	36.38092226906294	38.0	35.0	40.0	31.0	40.0
32	36.20626728792249	38.0	35.0	40.0	31.0	40.0
33	36.00473559647955	38.0	35.0	40.0	31.0	40.0
34	35.6287160712965	38.0	34.0	40.0	29.0	40.0
35	35.525746616374526	37.0	34.0	40.0	29.0	40.0
36	35.53415649730049	37.0	34.0	40.0	30.0	40.0
37	35.46507802677317	37.0	34.0	40.0	30.0	40.0
38	35.40338880260336	37.0	34.0	40.0	30.0	40.0
39	35.271616004733374	36.0	34.0	40.0	30.0	40.0
40	34.970124990755124	36.0	34.0	39.0	29.0	40.0
41	34.86471045041047	35.0	34.0	39.0	29.0	40.0
42	34.67766955106871	35.0	33.0	39.0	28.0	40.0
43	34.57614081798683	35.0	33.0	39.0	28.0	40.0
44	34.368243473115896	35.0	33.0	39.0	28.0	40.0
45	34.226679239701205	35.0	33.0	38.0	27.0	40.0
46	34.04110346867835	35.0	33.0	38.0	27.0	40.0
47	33.80520597588936	35.0	33.0	38.0	26.0	40.0
48	33.57571111604171	35.0	33.0	37.0	26.0	40.0
49	33.924910879372824	35.0	33.0	37.0	27.0	40.0
50	34.01628134013757	35.0	33.0	37.0	28.0	40.0
51	34.0006027660676	35.0	33.0	37.0	28.0	40.0
52	33.60057983876932	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	21.0
16	46.0
17	127.0
18	247.0
19	461.0
20	913.0
21	1633.0
22	2473.0
23	3554.0
24	5463.0
25	7487.0
26	9864.0
27	13184.0
28	16602.0
29	21073.0
30	26715.0
31	34527.0
32	45876.0
33	67442.0
34	140375.0
35	143000.0
36	180025.0
37	247397.0
38	300636.0
39	82955.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.4479698247171	0.4801420013312625	0.059463057466163746	0.012425116485467052
8	99.27623696472155	0.5264403520449671	0.11345314695658604	0.08386953627690259
9	98.69329191627838	0.7612602618149545	0.25375342060498485	0.2916944013016789
10	67.7105243694993	24.34109903113675	2.8080763257155534	5.140300273648399
11	41.596775386435915	23.491235855336143	18.05694845055839	16.85504030766955
12	35.01375637896606	15.911618963094446	24.181051697359663	24.89357296057984
13	27.442792692848162	16.060868278973448	27.284446416685153	29.211892611493234
14	27.53487168108868	16.491235855336146	29.637674728200576	26.3362177353746
15	29.227645884180163	17.089564381332742	27.137267953553728	26.545521780933363
16	34.60587234671992	17.94046298350714	23.61008801124177	23.843576658531173
17	34.930552473929446	18.694105465572072	22.393757858146586	23.981584202351897
18	32.98224983359219	20.07388506767251	22.976333111456253	23.96753198727905
19	33.12328969750758	22.122993861400783	20.327268693144	24.426447747947638
20	33.042156645218554	22.1402263146217	20.1680349086606	24.649582131499148
21	32.91775756231048	21.6168922416981	22.372309740403818	23.093040455587605
22	31.981880038458694	19.076843428740478	25.699430515494413	23.24184601730641
23	32.90215220767695	20.137193994527035	22.758893572960577	24.201760224835443
24	33.43768952000591	19.055469270024407	22.500702610753642	25.006138599216033
25	30.962872568597	19.778122919902373	21.167221359366913	28.091783152133722
26	27.859625767324903	19.276163005694848	23.912358553361436	28.951852673618816
27	25.96442570815768	18.413504918275276	23.65697803416907	31.965091339397972
28	28.340285481843058	22.66829376525405	20.97293099622809	28.018489756674803
29	31.386066119369872	21.16189630944457	21.46054285925597	25.99149471192959
30	29.6730271429628	20.111826048369203	23.782486502477624	26.43266030619037
31	34.5235559500037	20.13275645292508	20.437023888765623	24.906663708305597
32	35.87530508098513	19.80489608756749	21.054507802677318	23.26529102877006
33	35.360846091265444	19.07551216625989	22.63382885881222	22.92981288366245
34	31.213815546187412	21.335552104134308	26.414540344649062	21.036092005029214
35	28.73522668441683	25.149249315879	25.172176614155756	20.943347385548407
36	28.852525700761777	26.252274240071	22.099105095776938	22.79609496339028
37	31.796686635603876	26.234302196583094	19.59928999334369	22.369721174469344
38	32.366319059241185	25.711485836846386	20.1400783965683	21.78211670734413
39	33.6264329561423	22.41195177871459	19.614599511870423	24.34701575327269
40	28.873382146290954	23.21862288292286	20.445233340729235	27.46276163005695
41	27.441609348420975	23.832334886472893	21.20301752828933	27.523038236816806
42	25.319059241180387	21.92441387471341	21.4112861474743	31.345240736631908
43	26.304489312920644	19.6168922416981	24.345684490792102	29.732933954589157
44	26.843502699504473	19.482582649212336	23.10080615339102	30.573108497892164
45	29.04585459655351	21.275127579321058	22.307891428148803	27.37112639597663
46	28.2990163449449	24.07284964129872	21.285112047925452	26.343021965830932
47	26.452777161452556	21.535167517195475	24.067598550403076	27.944456770948893
48	27.96967679905332	20.98602174395385	25.555358331484356	25.488943125508467
49	29.47193254936765	20.043561866725835	24.10568744915317	26.378818134753345
50	26.468382516086088	20.822868131055394	24.94682345980327	27.761925893055245
51	24.995488499371348	21.043487907699134	25.236520967384067	28.72450262554545
52	26.27290880852008	20.11271355668959	25.71577546039494	27.898602174395386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	32.5
10	65.0
11	43.5
12	22.0
13	24.5
14	185.5
15	344.0
16	663.5
17	983.0
18	1221.0
19	1459.0
20	1327.5
21	1196.0
22	1068.5
23	941.0
24	1231.0
25	1521.0
26	1575.0
27	1629.0
28	1995.5
29	2362.0
30	2594.5
31	2827.0
32	3709.0
33	4591.0
34	5166.5
35	5742.0
36	6720.5
37	7699.0
38	8019.5
39	10934.5
40	13529.0
41	15726.5
42	17924.0
43	19289.5
44	20655.0
45	22361.0
46	24067.0
47	27681.0
48	31295.0
49	33581.0
50	35867.0
51	36867.0
52	37867.0
53	43040.5
54	48214.0
55	51469.5
56	54725.0
57	62462.5
58	70200.0
59	75204.0
60	80208.0
61	93870.5
62	107533.0
63	119571.5
64	132703.5
65	133797.0
66	145007.5
67	156218.0
68	131353.5
69	106489.0
70	92975.5
71	79462.0
72	66001.0
73	52540.0
74	45364.0
75	38188.0
76	31957.5
77	25727.0
78	27514.5
79	29302.0
80	20606.0
81	11910.0
82	7502.5
83	3095.0
84	2108.5
85	1122.0
86	874.0
87	626.0
88	365.0
89	77.5
90	51.0
91	34.5
92	18.0
93	13.0
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1352100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.417760383768952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.60136649188374	4.940329778683722
2	10.14491325998756	1.505050713534161
3	4.34342896827431	0.9665554599173889
4	2.357424924970756	0.6994725286463028
5	1.3216702930558746	0.4901916770117084
6	0.7728070043830553	0.3439498308847072
7	0.4888779044234012	0.2538465406352323
8	0.3769058688085161	0.22366379376462633
9	0.3259186027258396	0.21758274896697583
>10	5.2286941480163485	11.2612792020544
>50	4.883780289303973	26.137665372965152
>100	3.005249447215505	37.96069962731065
>500	0.09497627993528708	4.946410823474199
>1k	0.048987765440305975	6.321023582453476
>5k	0.003999001260433141	2.409057805105838
>10k+	9.997503151082852E-4	1.3232205145914564
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17843	1.3196509133939798	No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8756	0.6475852377782708	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8756	0.6475852377782708	No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7713	0.5704459729309963	No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7260	0.5369425338362547	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4964	0.3671326085348717	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4709	0.3482730567265735	No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3657	0.270468160639006	No Hit
GGGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC	3512	0.25974410176762075	No Hit
GGGCGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3361	0.24857628873604024	No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2862	0.21167073441313514	No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2685	0.1985799866873752	No Hit
GGGCGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2539	0.18778196878929074	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGTCTGCT	2043	0.15109829154648324	No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2017	0.14917535685230382	No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1975	0.14606907773093708	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC	1884	0.13933880630130907	No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1862	0.1377117077139265	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT	1853	0.13704607647363362	No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1723	0.12743140300273648	No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1722	0.1273574439760373	No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1639	0.12121884476000297	No Hit
GGGCGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1639	0.12121884476000297	No Hit
GGGCGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	1514	0.11197396642260188	No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1495	0.11056874491531693	No Hit
GGGCGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1476	0.10916352340803194	No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1466	0.10842393314103986	No Hit
GGGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGTGCCGACTGCT	1427	0.10553953109977071	No Hit
GGGCGAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1424	0.1053176540196731	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1383	0.10228533392500555	No Hit
GGGCGAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1362	0.10073219436432218	No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1359	0.10051031728422453	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.09170919310701871	0.0	0.0	0.0	0.0
7	0.09207898824051476	0.0	0.0	0.0	0.0
8	0.0951113083351823	0.0	0.0	0.0	0.0
9	0.10679683455365728	0.0	0.0	0.0	0.0
10	0.1291324606168183	0.0	0.0	0.0	0.0
11	0.16825678574069966	0.0	0.0	0.0	0.0
12	0.1764662377043118	0.0	0.0	0.0	0.0
13	0.18608091117520895	0.0	0.0	0.0	0.0
14	0.19917165890096886	0.0	0.0	0.0	0.0
15	0.21255824273352564	0.0	0.0	0.0	0.0
16	0.22490940019229347	0.0	0.0	0.0	0.0
17	0.23585533614377635	0.0	0.0	0.0	0.0
18	0.24717106722875526	0.0	0.0	0.0	0.0
19	0.26381184823607723	0.0	0.0	0.0	0.0
20	0.2785296945492197	0.0	0.0	0.0	0.0
21	0.289253753420605	0.0	0.0	0.0	0.0
22	0.30182678795947043	0.0	0.0	0.0	0.0
23	0.3167665113527106	0.0	0.0	0.0	0.0
24	0.3305228903187634	0.0	0.0	0.0	0.0
25	0.34206049848383996	0.0	0.0	0.0	0.0
26	0.3553731232896975	0.0	0.0	0.0	0.0
27	0.3662451002144812	0.0	0.0	0.0	0.0
28	0.3840692256489905	0.0	0.0	0.0	0.0
29	0.39494120257377413	0.0	0.0	0.0	0.0
30	0.4067006878189483	0.0	0.0	0.0	0.0
31	0.41772058279713037	0.0	0.0	0.0	0.0
32	0.43103320760298797	0.0	0.0	0.0	0.0
33	0.44227497966126766	0.0	0.0	0.0	0.0
34	0.4549219732268323	0.0	0.0	0.0	0.0
35	0.4700096146734709	0.0	0.0	0.0	0.0
36	0.4855410102803047	0.0	0.0	0.0	0.0
37	0.500850528807041	0.0	0.0	0.0	0.0
38	0.5145329487463945	0.0	0.0	0.0	0.0
39	0.5254049256711781	0.0	0.0	0.0	0.0
40	0.5370164928629539	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	20	6.311852E-4	46.0	37
AGCGTAC	20	6.311852E-4	46.0	11
CATATCG	25	3.417478E-5	46.0	22
GGTCGAC	30	1.8616483E-6	46.0	9
ACGTTAT	20	6.311852E-4	46.0	38
CGCATTC	20	6.311852E-4	46.0	45
TAGCGTC	20	6.311852E-4	46.0	22
GACGTAT	20	6.311852E-4	46.0	10
TAACGCA	20	6.311852E-4	46.0	27
ATCACGC	20	6.311852E-4	46.0	43
ATATCGT	30	1.8616483E-6	46.0	23
TACGATA	30	1.8616483E-6	46.0	31
TAACACG	20	6.311852E-4	46.0	42
TATTCGC	20	6.311852E-4	46.0	12
TCGTATT	55	1.8189894E-12	46.0	22
ACGTATC	25	3.417478E-5	46.0	14
ATAACCG	20	6.311852E-4	46.0	11
AACTCGT	20	6.311852E-4	46.0	20
TCATACG	25	3.417478E-5	46.0	41
ATACGTA	25	3.417478E-5	46.0	43
>>END_MODULE