FastQCFastQC Report
Fri 17 Jun 2016
SRR1527796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361067
Sequences flagged as poor quality0
Sequence length52
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC169841.2478445219816512No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT85460.6278897365081954No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG83450.6131219109713189No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC75750.5565486489643787No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT69520.5107757369769453No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC46470.3414233098003258No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC46140.33899874142859976No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34770.2554613402573128No Hit
GGGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC33420.24554265146388823No Hit
GGGCGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT31980.23496271675090205No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC27750.20388415853150507No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG24250.17816903943744136No Hit
GGGCGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23880.17545058399035462No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGTCTGCT19590.14393119515791655No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT18950.13922900195214488No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC18820.13827386895722255No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT18590.13658401827389835No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC17760.13048586146016325No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT16680.12255091042542357No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16640.12225702335006286No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT16410.12056717266673866No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC15640.11490984646604464No Hit
GGGCGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG15270.11219139101895793No Hit
GGGCGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15070.11072195564215427No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG14490.10646059304942372No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC14400.1057993471298621No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT14170.10410949644653791No Hit
GGGCGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA13830.10161145630597171No Hit
GGGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGTGCCGACTGCT13730.10087673861756989No Hit
GGGCGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT13670.10043590800452878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA253.417491E-546.037
AGCGTAA253.417491E-546.032
CCGATTA301.8616574E-646.032
CCGATAG206.3118665E-446.027
TAGCGTA253.417491E-546.029
CAACCCG301.8616574E-646.038
TAACGCA301.8616574E-646.027
ACGATAA253.417491E-546.032
ACTAGCA206.3118665E-446.019
TTAAGCG253.417491E-546.046
TCGTATT405.6134013E-946.022
GCGATCA600.046.019
GCGATAA253.417491E-546.031
TTAACCG301.8616574E-646.038
CCAACGA301.8616574E-646.043
GACGAAT206.3118665E-446.09
GATTCGT206.3118665E-446.09
CAATACG206.3118665E-446.028
CGATAGG206.3118665E-446.021
TAATGCG206.3118665E-446.013
Produced by FastQC (version 0.11.5)