##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527796_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1361067
Sequences flagged as poor quality	0
Sequence length	52
%GC	62
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.096271528146666	33.0	31.0	34.0	31.0	34.0
2	32.72760856004884	34.0	31.0	34.0	31.0	34.0
3	32.92258059302004	34.0	31.0	34.0	31.0	34.0
4	36.104704617774146	37.0	37.0	37.0	35.0	37.0
5	36.20259472898836	37.0	37.0	37.0	35.0	37.0
6	35.198364224538544	37.0	35.0	37.0	32.0	37.0
7	36.24534207353496	37.0	35.0	37.0	35.0	37.0
8	36.55896807431228	37.0	37.0	37.0	35.0	37.0
9	38.64212783059173	39.0	39.0	39.0	38.0	39.0
10	37.96133474693016	39.0	38.0	39.0	35.0	39.0
11	37.62594273463393	39.0	37.0	39.0	35.0	39.0
12	36.88163551096309	39.0	35.0	39.0	33.0	39.0
13	36.63768572744765	39.0	35.0	39.0	33.0	39.0
14	37.54428547602726	40.0	36.0	40.0	33.0	40.0
15	37.61572868932977	40.0	36.0	40.0	33.0	40.0
16	37.6364095228229	40.0	36.0	40.0	33.0	40.0
17	37.47862742980323	40.0	36.0	40.0	33.0	40.0
18	37.47313688451781	40.0	36.0	40.0	33.0	40.0
19	37.393604429466	40.0	36.0	40.0	33.0	40.0
20	37.37299927189477	40.0	35.0	40.0	33.0	40.0
21	37.40549510053509	40.0	35.0	40.0	33.0	40.0
22	37.29197460521782	40.0	35.0	40.0	33.0	40.0
23	37.279890703396674	40.0	35.0	40.0	33.0	40.0
24	37.241420150514266	39.0	35.0	40.0	33.0	40.0
25	37.19549221309458	39.0	35.0	40.0	33.0	40.0
26	37.01067912160092	39.0	35.0	40.0	33.0	40.0
27	36.89322347834457	39.0	35.0	40.0	32.0	40.0
28	36.76020871860092	39.0	35.0	40.0	32.0	40.0
29	36.6398369808393	38.0	35.0	40.0	32.0	40.0
30	36.4253670098533	38.0	35.0	40.0	31.0	40.0
31	36.34217198712481	38.0	35.0	40.0	31.0	40.0
32	36.17392677950461	38.0	35.0	40.0	31.0	40.0
33	35.79731196186521	38.0	35.0	40.0	30.0	40.0
34	35.53362913067468	38.0	34.0	40.0	28.0	40.0
35	35.513206917807864	37.0	34.0	40.0	29.0	40.0
36	35.37882925675224	37.0	34.0	40.0	29.0	40.0
37	35.37112794594241	37.0	34.0	40.0	29.0	40.0
38	35.047523744238894	36.0	34.0	40.0	29.0	40.0
39	35.08130826770468	36.0	34.0	40.0	29.0	40.0
40	34.85151722876243	36.0	34.0	39.0	28.0	40.0
41	34.56492883891829	35.0	33.0	39.0	28.0	40.0
42	34.512311296945704	35.0	33.0	39.0	28.0	40.0
43	34.51922205152281	35.0	33.0	39.0	28.0	40.0
44	34.31060263748956	35.0	33.0	39.0	28.0	40.0
45	34.17253155061434	35.0	33.0	38.0	27.0	40.0
46	33.815686516534456	35.0	33.0	38.0	26.0	40.0
47	33.50172842336197	35.0	33.0	38.0	25.0	40.0
48	33.42849984607665	35.0	32.0	37.0	25.0	40.0
49	33.37867496603767	35.0	32.0	37.0	26.0	40.0
50	33.231896005119516	35.0	32.0	37.0	26.0	40.0
51	33.56342560652782	35.0	33.0	37.0	27.0	40.0
52	33.37496317227587	35.0	33.0	36.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	6.0
15	18.0
16	41.0
17	118.0
18	299.0
19	536.0
20	1019.0
21	1649.0
22	2593.0
23	3833.0
24	5628.0
25	7893.0
26	10622.0
27	13875.0
28	17377.0
29	22006.0
30	27966.0
31	36393.0
32	49070.0
33	73621.0
34	144378.0
35	146497.0
36	185575.0
37	250509.0
38	287406.0
39	72138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.43536945646319	0.4866769967973656	0.06458168481051998	0.013371861928913124
8	99.25896373947792	0.5446462224122692	0.11108931448635519	0.08530072362345131
9	98.6507644370189	0.7785803343994087	0.2733884518543172	0.2972667767273764
10	67.66456023105401	24.347589060641393	2.8355694466179844	5.152281261686603
11	41.616907911219656	23.44910279949481	18.047678769671148	16.88631051961439
12	35.00452218737211	15.918907739295715	24.18014689945462	24.89642317387755
13	27.623474817918588	16.10714241106426	27.146716509914647	29.122666261102502
14	27.500630020417805	16.569794139450885	29.661287798469875	26.26828804166143
15	29.11002911686199	17.11870172445589	27.13018536192561	26.641083796756515
16	34.56148742126581	17.909992674864647	23.69942111593331	23.82909878793623
17	34.91907452021098	18.708483858619747	22.43548627657566	23.936955344593617
18	32.942169636028204	20.058013308676205	22.955813343501827	24.044003711793764
19	33.03158477870671	22.051228925541505	20.465561210432696	24.451625085319094
20	33.064647074684785	22.0104520938352	20.187764452447972	24.737136379032037
21	32.8333579463759	21.719871248072284	22.37501901082019	23.071751794731632
22	32.057936898036616	19.068128167092436	25.62225077824971	23.251684156621238
23	32.82380661642667	20.146546790128628	22.825107066735143	24.20453952670956
24	33.51326569522294	18.968941279158187	22.562739380206853	24.95505364541202
25	30.98627767773372	19.806445972167424	21.188082585207045	28.01919376489181
26	27.894585644938864	19.269073454870334	23.90881565712783	28.92752524306298
27	25.957061628854422	18.39931465534026	23.665697574035665	31.977926141769657
28	28.414545353020827	22.69050678621993	20.929168071814246	27.965779788944996
29	31.415279335991542	21.142970919139177	21.46448337958381	25.977266365285473
30	29.628519389567153	20.07454445666525	23.823221046429016	26.47371510733858
31	34.562148667185376	20.01870591234671	20.51552201324402	24.90362340722389
32	35.92078861657802	19.70101398388176	21.113435268065423	23.264762131474793
33	35.31663026140521	19.107509035190773	22.57001308532203	23.00584761808199
34	31.24056346968959	21.353173649790936	26.35248668875228	21.053776191767195
35	28.758540174730562	25.163419581842778	25.13682280152263	20.941217441904037
36	28.992915117330742	26.217445577624026	22.090462850102163	22.69917645494307
37	31.82326806836107	26.316926352633637	19.62239919122277	22.237406387782528
38	32.43727164055847	25.724670424012924	20.016575231050346	21.821482704378255
39	33.71935400681965	22.380676337020883	19.572732275486807	24.327237380672663
40	28.903940805265282	23.225969037527175	20.472100197859476	27.397989959348067
41	27.691583147633438	23.795301774269745	21.109688207854575	27.403426870242242
42	25.293023782076858	21.894881001449598	21.41268578255148	31.399409433922067
43	26.403769983402725	19.64818778208567	24.334364142250163	29.61367809226144
44	26.84856807196119	19.395151010200085	23.128765887351616	30.627515030487114
45	29.038467614011655	21.292779855804305	22.36025118528331	27.308501344900726
46	28.518360962392002	24.069424943812464	21.197780858693953	26.21443323510158
47	26.659304795428877	21.614145372711263	24.008443375675114	27.718106456184742
48	27.952995701166806	20.999627498131982	25.563620306715247	25.48375649398597
49	29.49751922572511	20.00724431640764	24.110936493207166	26.38429996466008
50	26.57855932147352	20.73292497724212	24.93293864299112	27.755577058293234
51	25.08943351062071	21.012778944754373	25.2141151023425	28.683672442282415
52	26.307301550915568	20.100259575759313	25.733119677429546	27.859319195895573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	35.0
10	70.0
11	50.0
12	30.0
13	27.0
14	179.5
15	335.0
16	665.5
17	996.0
18	1252.0
19	1508.0
20	1363.5
21	1219.0
22	1131.0
23	1043.0
24	1227.0
25	1411.0
26	1569.0
27	1727.0
28	2072.0
29	2417.0
30	2612.5
31	2808.0
32	3664.5
33	4521.0
34	5228.0
35	5935.0
36	6652.5
37	7370.0
38	7837.5
39	11023.0
40	13741.0
41	15878.0
42	18015.0
43	19433.0
44	20851.0
45	22505.5
46	24160.0
47	27656.5
48	31153.0
49	33628.0
50	36103.0
51	37004.5
52	37906.0
53	42877.0
54	47848.0
55	51730.0
56	55612.0
57	63447.0
58	71282.0
59	76384.5
60	81487.0
61	94792.5
62	108098.0
63	120254.0
64	133388.0
65	134366.0
66	145449.5
67	156533.0
68	132154.0
69	107775.0
70	93827.0
71	79879.0
72	66501.0
73	53123.0
74	45678.5
75	38234.0
76	32355.5
77	26477.0
78	27821.5
79	29166.0
80	20593.0
81	12020.0
82	7556.0
83	3092.0
84	2149.0
85	1206.0
86	915.0
87	624.0
88	368.5
89	79.5
90	46.0
91	32.5
92	19.0
93	11.5
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1361067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.77913670544746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9913625679598	7.867838754369191
2	9.92015669451088	2.1386145029918513
3	3.655693749765047	1.1821566804590182
4	1.7840739971957655	0.7692311003362897
5	0.9482199998491523	0.5110496502606691
6	0.5837367936184213	0.3775307219047521
7	0.405176671982245	0.3057218316008404
8	0.2725988602905574	0.2350704304656873
9	0.23189045846969703	0.2249621057280376
>10	3.8874408533492986	12.060082877523023
>50	3.3563389620240205	26.002574604501493
>100	1.864250581489054	34.18687993751537
>500	0.06328821162250663	4.656926311953743
>1k	0.032332021154976215	5.892287558345625
>5k	0.0027516613748915925	2.3296866530096128
>10k+	6.879153437228981E-4	1.2593862790347974
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16984	1.2478445219816512	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8546	0.6278897365081954	No Hit
GGGCGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8345	0.6131219109713189	No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7575	0.5565486489643787	No Hit
GGGCGAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6952	0.5107757369769453	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4647	0.3414233098003258	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4614	0.33899874142859976	No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3477	0.2554613402573128	No Hit
GGGCGAGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTC	3342	0.24554265146388823	No Hit
GGGCGAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3198	0.23496271675090205	No Hit
GGGCGAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2775	0.20388415853150507	No Hit
GGGCGAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2425	0.17816903943744136	No Hit
GGGCGAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2388	0.17545058399035462	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGTCTGCT	1959	0.14393119515791655	No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1895	0.13922900195214488	No Hit
GGGCGAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1882	0.13827386895722255	No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1859	0.13658401827389835	No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTC	1776	0.13048586146016325	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGCAGGCAAGAGCACCCGACTGCT	1668	0.12255091042542357	No Hit
GGGCGAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1664	0.12225702335006286	No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1641	0.12056717266673866	No Hit
GGGCGAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1564	0.11490984646604464	No Hit
GGGCGAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG	1527	0.11219139101895793	No Hit
GGGCGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1507	0.11072195564215427	No Hit
GGGCGAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1449	0.10646059304942372	No Hit
GGGCGAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1440	0.1057993471298621	No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1417	0.10410949644653791	No Hit
GGGCGAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	1383	0.10161145630597171	No Hit
GGGCGAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGTGCCGACTGCT	1373	0.10087673861756989	No Hit
GGGCGAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1367	0.10043590800452878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.09551329949223661	0.0	0.0	0.0	0.0
7	0.0963949607183188	0.0	0.0	0.0	0.0
8	0.09977466208496716	0.0	0.0	0.0	0.0
9	0.11086889917983464	0.0	0.0	0.0	0.0
10	0.13423292167101253	0.0	0.0	0.0	0.0
11	0.1739076768447108	0.0	0.0	0.0	0.0
12	0.18323859148741392	0.0	0.0	0.0	0.0
13	0.19425935681344123	0.0	0.0	0.0	0.0
14	0.20682302928511234	0.0	0.0	0.0	0.0
15	0.21938670175678346	0.0	0.0	0.0	0.0
16	0.23077482592701168	0.0	0.0	0.0	0.0
17	0.2425303089414408	0.0	0.0	0.0	0.0
18	0.2526694130413859	0.0	0.0	0.0	0.0
19	0.27081693994491085	0.0	0.0	0.0	0.0
20	0.2830132535723811	0.0	0.0	0.0	0.0
21	0.29440137774260927	0.0	0.0	0.0	0.0
22	0.30564255837515714	0.0	0.0	0.0	0.0
23	0.31945525091711136	0.0	0.0	0.0	0.0
24	0.3326801693083441	0.0	0.0	0.0	0.0
25	0.34399482170973217	0.0	0.0	0.0	0.0
26	0.3569258530256042	0.0	0.0	0.0	0.0
27	0.3669180135878689	0.0	0.0	0.0	0.0
28	0.3838165204211108	0.0	0.0	0.0	0.0
29	0.3968944952746632	0.0	0.0	0.0	0.0
30	0.4087969218267727	0.0	0.0	0.0	0.0
31	0.4193033847709187	0.0	0.0	0.0	0.0
32	0.432748718468672	0.0	0.0	0.0	0.0
33	0.4425939354932564	0.0	0.0	0.0	0.0
34	0.45302692666856226	0.0	0.0	0.0	0.0
35	0.46823558281847993	0.0	0.0	0.0	0.0
36	0.4854279767270825	0.0	0.0	0.0	0.0
37	0.5027673141733655	0.0	0.0	0.0	0.0
38	0.5163595914087992	0.0	0.0	0.0	0.0
39	0.5279681308855478	0.0	0.0	0.0	0.0
40	0.5394297268246163	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	25	3.417491E-5	46.0	37
AGCGTAA	25	3.417491E-5	46.0	32
CCGATTA	30	1.8616574E-6	46.0	32
CCGATAG	20	6.3118665E-4	46.0	27
TAGCGTA	25	3.417491E-5	46.0	29
CAACCCG	30	1.8616574E-6	46.0	38
TAACGCA	30	1.8616574E-6	46.0	27
ACGATAA	25	3.417491E-5	46.0	32
ACTAGCA	20	6.3118665E-4	46.0	19
TTAAGCG	25	3.417491E-5	46.0	46
TCGTATT	40	5.6134013E-9	46.0	22
GCGATCA	60	0.0	46.0	19
GCGATAA	25	3.417491E-5	46.0	31
TTAACCG	30	1.8616574E-6	46.0	38
CCAACGA	30	1.8616574E-6	46.0	43
GACGAAT	20	6.3118665E-4	46.0	9
GATTCGT	20	6.3118665E-4	46.0	9
CAATACG	20	6.3118665E-4	46.0	28
CGATAGG	20	6.3118665E-4	46.0	21
TAATGCG	20	6.3118665E-4	46.0	13
>>END_MODULE