FastQCFastQC Report
Fri 17 Jun 2016
SRR1527795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337932
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGA16390.48500881834215165No Hit
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT16390.48500881834215165No Hit
ACACCCGGGATGTTTCAGGATTCTTTTAACCCTGTTCCAAACTGAGTGTA14180.4196110460092563No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11770.34829492323899486No Hit
ACACCCGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAAC11270.3334990471455796No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG10170.3009481197400661No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA8430.249458470934981No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC7520.22252997644496525No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT7120.21069327557023307No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA6870.20329533752352544No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC6690.19796882212989594No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG6500.19234638921439814Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT5840.17281583277109003No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT5320.15742812163393818No Hit
ACACCCCCCAGTCTGTCCCATCTCAGTTTGCAAAAAGCACTGACACGTAT5260.15565261650272835No Hit
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCT5210.15417302889338685Illumina Single End Adapter 2 (96% over 29bp)
ACACCCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG5190.15358119384965022No Hit
ACACCCGGGGTACAGCCTTGAGTGTGAATAATTATTGTAAACTATATTTT4970.14707100836854753No Hit
ACACCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA4960.14677509084667922No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG4740.1402649053655765No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC4500.1331628848407372No Hit
ACACCCGGGGTGTGTGTGTGTGTGTATCTCTGTGTGTATGTGTGTTTCTG4380.12961187457831755No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGT4290.1269486168815028No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC4270.1263567818377662No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG4130.12221393653160992No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC4080.12073434892226839No Hit
ACACCCGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA4050.11984659635666348No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG3930.1162955860942438No Hit
ACACCCGGGAGGGGGTATGGGGCTGCTGCTGGATCCCGCGCTCCTAGCAT3820.11304049335369246No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT3820.11304049335369246No Hit
ACACCCGGGGAGCGTGGTCACGTGGCCGCTGGTCACCGCCGCCACCTCCC3740.11067315317874601No Hit
ACACCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA3590.10623439035072145No Hit
ACACCCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAG3510.10386705017577502No Hit
ACACCCGGGAGCTTTCAGTGCAAAGAGCCACATCCAGGCCTGAGGGCAGC3490.1032752151320384No Hit
ACACCCGGGGTCTGGGCAGCTTTCCCTAGCAGAGGCTCTTTGGCTTCTTT3470.10268338008830179No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT3460.1023874625664335No Hit
ACACCCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC3440.10179562752269689No Hit
ACACCCGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3440.10179562752269689No Hit
ACACCCGGGACGATGGGTGCCACCCCTCATTGGCGAGATGTATGGGTTGC3430.10149971000082857No Hit
ACACCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.10002012239148704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC254.4361484E-544.028
CTTGTCG207.848584E-444.019
TTGATAC207.848584E-444.013
ATCCTCG207.848584E-444.030
TGTCAAT302.5223926E-644.037
CGGGTAT600.044.06
TTAGGCT254.4361484E-544.018
AGACTAC207.848584E-444.043
GTCGCTC207.848584E-444.022
TGATACT254.4361484E-544.014
AATCCGG207.848584E-444.021
AATCCGA207.848584E-444.029
GTCGCAT207.848584E-444.038
ACACGTA950.044.043
CTCGTTC454.783942E-1044.044
ATTAGTG207.848584E-444.026
ATAGGTC551.8189894E-1244.038
CACGGGT408.281859E-944.016
CGAAACT207.848584E-444.022
CTATCGA207.848584E-444.041
Produced by FastQC (version 0.11.5)