SRR1527794_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427164
Sequences flagged as poor quality	0
Sequence length	52
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2145	0.502149057504846	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG	1881	0.4403460965811725	No Hit
ACACCCGGGATGTTTCAGGATTCTTTTAACCCTGTTCCAAACTGAGTGTAAT	1863	0.4361322583363767	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1631	0.3818205654034516	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1305	0.30550327274770345	No Hit
ACACCCGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT	1236	0.28935022614265243	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1040	0.24346620969931923	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	993	0.2324634098379077	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	963	0.22544034609658117	No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	855	0.20015731662780573	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	794	0.18587708702044178	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	748	0.17510838928374114	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	700	0.16387148729761872	No Hit
ACACCCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	689	0.161296363925799	No Hit
ACACCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	668	0.15638021930687043	No Hit
ACACCCGGGGTACAGCCTTGAGTGTGAATAATTATTGTAAACTATATTTTAC	658	0.15403919805976157	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	596	0.1395248663276868	No Hit
ACACCCCCCAGTCTGTCCCATCTCAGTTTGCAAAAAGCACTGACACGTATCT	595	0.1392907642029759	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	579	0.13554513020760176	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	567	0.13273590471107116	No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	560	0.13109718983809496	Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGTCTGGGCAGCTTTCCCTAGCAGAGGCTCTTTGGCTTCTTTCT	540	0.1264151473438773	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	538	0.12594694309445553	No Hit
ACACCCGGGGAGCGTGGTCACGTGGCCGCTGGTCACCGCCGCCACCTCCCGC	529	0.12384002397205757	No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	507	0.11868977722841811	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	504	0.11798747085428549	No Hit
ACACCCGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT	504	0.11798747085428549	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	502	0.11751926660486371	No Hit
ACACCCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA	488	0.11424183685891133	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGC	469	0.10979389648940455	No Hit
ACACCCGGGACGATGGGTGCCACCCCTCATTGGCGAGATGTATGGGTTGCGC	457	0.10698467099287393	No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT	453	0.10604826249403039	No Hit
ACACCCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	452	0.10581416036931951	No Hit
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC	447	0.1046436497457651	Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	439	0.10277083274807801	No Hit
ACACCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	432	0.10113211787510182	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACTA	35	1.0175427E-7	46.000004	32
ACACGCG	35	1.0175427E-7	46.000004	12
GCGGTCA	35	1.0175427E-7	46.000004	16
TATATAG	35	1.0175427E-7	46.000004	36
ATATAGG	35	1.0175427E-7	46.000004	37
CGTCGTA	35	1.0175427E-7	46.000004	35
CGGTCAG	35	1.0175427E-7	46.000004	17
CGGTAAC	70	0.0	46.000004	20
GTAACGG	35	1.0175427E-7	46.000004	37
TGCCGAT	35	1.0175427E-7	46.000004	15
ACAACTC	30	1.8586361E-6	46.0	41
TTTAGTA	20	6.3069805E-4	46.0	23
AACGTTC	20	6.3069805E-4	46.0	18
AGACTAC	50	1.6370905E-11	46.0	43
CCTTATA	20	6.3069805E-4	46.0	26
TCGTTTA	25	3.4135264E-5	46.0	28
TCGTTGT	25	3.4135264E-5	46.0	23
ATCTCGT	20	6.3069805E-4	46.0	30
GTCGCAT	20	6.3069805E-4	46.0	38
GCAAGTA	20	6.3069805E-4	46.0	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content