##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427164
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06139562322667	31.0	31.0	34.0	31.0	34.0
2	32.509331310690975	34.0	31.0	34.0	31.0	34.0
3	32.46503450665318	34.0	31.0	34.0	31.0	34.0
4	36.22320467080559	37.0	37.0	37.0	35.0	37.0
5	36.1647353241378	37.0	37.0	37.0	35.0	37.0
6	36.305486885598974	37.0	37.0	37.0	35.0	37.0
7	36.400092236237136	37.0	37.0	37.0	35.0	37.0
8	36.5401227631542	37.0	37.0	37.0	35.0	37.0
9	38.62649474206628	39.0	39.0	39.0	38.0	39.0
10	37.941050743976554	39.0	38.0	39.0	35.0	39.0
11	37.69739959359871	39.0	38.0	39.0	35.0	39.0
12	37.22475676789242	39.0	37.0	39.0	34.0	39.0
13	37.08134112425204	39.0	37.0	39.0	33.0	39.0
14	37.96445159236265	40.0	38.0	40.0	33.0	40.0
15	37.989931267616186	40.0	38.0	40.0	33.0	40.0
16	37.91687969960015	40.0	38.0	40.0	33.0	40.0
17	37.94836175333128	40.0	38.0	40.0	34.0	40.0
18	37.96730295624163	40.0	38.0	40.0	34.0	40.0
19	37.72436113530166	40.0	37.0	40.0	33.0	40.0
20	37.78407356425167	40.0	37.0	40.0	33.0	40.0
21	37.7563113932822	40.0	37.0	40.0	33.0	40.0
22	37.7383815115506	40.0	37.0	40.0	33.0	40.0
23	37.71092133232201	40.0	37.0	40.0	33.0	40.0
24	37.63613272654062	40.0	37.0	40.0	33.0	40.0
25	37.535316646533886	40.0	36.0	40.0	33.0	40.0
26	37.38757947767134	40.0	36.0	40.0	33.0	40.0
27	37.2363143897894	40.0	35.0	40.0	33.0	40.0
28	37.12830903353279	39.0	35.0	40.0	32.0	40.0
29	37.04324334447659	39.0	35.0	40.0	32.0	40.0
30	36.847447350432155	39.0	35.0	40.0	32.0	40.0
31	36.70510389452295	39.0	35.0	40.0	31.0	40.0
32	36.40743367886807	39.0	35.0	40.0	31.0	40.0
33	36.296321787416545	38.0	35.0	40.0	31.0	40.0
34	36.1108075586894	38.0	35.0	40.0	30.0	40.0
35	36.03300371754174	38.0	35.0	40.0	30.0	40.0
36	35.939112378383946	38.0	35.0	40.0	30.0	40.0
37	35.68701014130404	38.0	35.0	40.0	30.0	40.0
38	35.59644539333839	37.0	34.0	40.0	30.0	40.0
39	35.45744257474881	37.0	34.0	40.0	29.0	40.0
40	35.239896152297476	37.0	34.0	40.0	29.0	40.0
41	35.08371023775412	37.0	34.0	40.0	28.0	40.0
42	34.973853133691044	36.0	34.0	40.0	28.0	40.0
43	34.82622599282711	36.0	34.0	40.0	28.0	40.0
44	34.601780580760554	36.0	34.0	40.0	27.0	40.0
45	34.532807071756984	35.0	33.0	39.0	27.0	40.0
46	34.36936165032634	35.0	33.0	39.0	26.0	40.0
47	34.13531805114663	35.0	33.0	39.0	26.0	40.0
48	33.92609630025002	35.0	33.0	39.0	26.0	40.0
49	34.16556404565928	35.0	33.0	39.0	27.0	40.0
50	34.10279424296055	35.0	33.0	39.0	27.0	40.0
51	34.07521467164836	35.0	33.0	39.0	27.0	40.0
52	33.613956232266766	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	1.0
15	6.0
16	11.0
17	35.0
18	57.0
19	124.0
20	254.0
21	455.0
22	835.0
23	1318.0
24	1859.0
25	2695.0
26	3579.0
27	4629.0
28	5603.0
29	6656.0
30	8209.0
31	10043.0
32	12814.0
33	17722.0
34	31284.0
35	38925.0
36	54229.0
37	76714.0
38	106943.0
39	42160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	97.6297159873023	1.2084351677575826	0.22473803972244852	0.9371108052176681
8	98.29714114485303	0.9043365077581444	0.20554166549615602	0.5929806818926688
9	96.95807699150677	1.719480106001442	0.54030770383272	0.7821351986590631
10	53.716605331909996	32.97843451227163	6.666760307516551	6.638199848301824
11	37.59539661581969	24.707606446236106	20.520924047906657	17.17607289003755
12	32.14620145892444	15.643874483804815	26.966691949696138	25.24323210757461
13	23.854538303789646	18.785290895300164	27.750465863228175	29.609704937682015
14	26.142652470713823	21.141528780515213	26.770982573437834	25.944836175333126
15	27.21507430401438	19.317639126892715	27.294669026416084	26.172617542676818
16	29.447706267382085	20.710780871047184	25.35255779981459	24.48895506175614
17	32.68159301813823	21.05912483261698	20.636102293264415	25.62317985598037
18	30.819544718187863	20.4443726531262	22.006067927072507	26.73001470161343
19	30.877133840866737	20.97414576134693	22.6641289996348	25.48459139815153
20	28.879540410708763	24.509087844481275	21.841260031276043	24.77011171353391
21	29.451451901377457	19.183030405183956	26.365517693438584	25.0
22	30.40237473195307	18.57717410643219	22.88910114148196	28.131350020132786
23	26.91027333764081	24.08325607963218	22.98180558286747	26.02466499985954
24	26.365283591313876	23.97744191926286	24.402805479862536	25.254469009560733
25	26.440430373346068	24.089810939124085	22.61332883857254	26.85642984895731
26	28.31839761777678	23.468269798016685	21.300250021069193	26.91308256313734
27	26.402271727018196	20.630483842271353	23.345366182543472	29.621878248166983
28	28.04122070211909	23.834171418939796	21.855306158758697	26.269301720182415
29	30.120983978050585	22.508216984577352	21.878014064855652	25.492784972516407
30	27.680235225814908	22.57399968162111	22.991871974230037	26.753893118333945
31	31.698598196477228	21.146679027258852	22.583129664484836	24.571593111779084
32	30.661291681883306	20.77867048721334	24.002022642357502	24.558015188545852
33	32.2227528537049	21.164236686612167	23.14146323192029	23.471547227762642
34	28.22616138064069	23.572913447762453	23.569167813767077	24.63175735782978
35	27.792136041426712	25.157784832055135	23.770261538893728	23.279817587624425
36	30.017276736803662	23.348175408040003	23.316805723328745	23.317742131827586
37	29.628901311908308	23.43502729630774	23.168150874137332	23.76792051764662
38	31.23039394705546	22.68238896536225	22.389293105224223	23.697923982358063
39	28.916060342163664	22.79054414697868	21.332322012154584	26.961073498703076
40	25.99750915339308	25.03745633995374	23.50525793372101	25.459776572932174
41	27.448474122351136	24.460394602541413	23.100261257971177	24.990870017136277
42	27.150930321843603	20.966888595480892	22.188667584346998	29.69351349832851
43	26.310503694131526	23.87490518863949	24.90729555861449	24.90729555861449
44	26.509022295886357	21.0724686537255	24.114859866468148	28.303649183919994
45	25.933599273347003	22.77837083649371	22.874586809749886	28.413443080409394
46	27.750699965352887	22.712588139449956	22.315316833815587	27.221395061381575
47	26.9357904692343	22.48597728272982	25.11166671348709	25.466565534548792
48	30.203387925948817	22.485509078480394	22.985785318987556	24.325317676583232
49	28.571227912464536	23.29760934910245	23.675918382635242	24.455244355797774
50	26.256894307572736	25.346002940322688	23.72835725857048	24.6687454935341
51	25.9808879025386	24.622861477090765	23.501746401850344	25.894504218520286
52	27.076719948310252	24.17596052101769	24.12586266632956	24.621456864342502
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	12.0
8	24.0
9	26.0
10	28.0
11	38.5
12	49.0
13	195.0
14	502.0
15	663.0
16	800.0
17	937.0
18	1004.5
19	1072.0
20	768.0
21	464.0
22	605.5
23	747.0
24	730.0
25	713.0
26	855.5
27	998.0
28	1012.0
29	1026.0
30	1299.0
31	1572.0
32	2093.5
33	2615.0
34	2549.5
35	2484.0
36	4013.5
37	5543.0
38	4820.0
39	4693.0
40	5289.0
41	7125.5
42	8962.0
43	9316.5
44	9671.0
45	11338.0
46	13005.0
47	14584.5
48	16164.0
49	15898.0
50	15632.0
51	17614.0
52	19596.0
53	20500.0
54	21404.0
55	24155.5
56	26907.0
57	29086.0
58	31265.0
59	33825.5
60	36386.0
61	36282.5
62	36179.0
63	36261.0
64	34424.0
65	32505.0
66	31354.0
67	30203.0
68	26671.5
69	23140.0
70	18101.5
71	13063.0
72	10958.5
73	8854.0
74	7762.0
75	6670.0
76	6864.5
77	7059.0
78	5504.5
79	3950.0
80	2397.5
81	845.0
82	682.5
83	520.0
84	280.0
85	40.0
86	84.0
87	128.0
88	68.0
89	4.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	427164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.589693888061728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8045528915103	7.85834012229495
2	11.281232956955582	2.614920733020573
3	5.0053527783949745	1.7403151951007108
4	2.6238713717252105	1.2163946399977528
5	1.405861797321591	0.8146753939938758
6	0.7817076373038157	0.5435851335786723
7	0.5312380067465208	0.43098201159273725
8	0.3211667036984669	0.2977779026322443
9	0.2989476235683843	0.31182403011489734
>10	4.633688165309956	15.337903006807691
>50	3.4641565839174255	28.783558539577303
>100	1.7916658250348434	34.26974183217687
>500	0.04241824388470317	3.181213772696201
>1k	0.014139414628234391	2.598767686415522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2145	0.502149057504846	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG	1881	0.4403460965811725	No Hit
ACACCCGGGATGTTTCAGGATTCTTTTAACCCTGTTCCAAACTGAGTGTAAT	1863	0.4361322583363767	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1631	0.3818205654034516	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1305	0.30550327274770345	No Hit
ACACCCGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT	1236	0.28935022614265243	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1040	0.24346620969931923	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	993	0.2324634098379077	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	963	0.22544034609658117	No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	855	0.20015731662780573	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	794	0.18587708702044178	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	748	0.17510838928374114	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	700	0.16387148729761872	No Hit
ACACCCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	689	0.161296363925799	No Hit
ACACCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	668	0.15638021930687043	No Hit
ACACCCGGGGTACAGCCTTGAGTGTGAATAATTATTGTAAACTATATTTTAC	658	0.15403919805976157	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	596	0.1395248663276868	No Hit
ACACCCCCCAGTCTGTCCCATCTCAGTTTGCAAAAAGCACTGACACGTATCT	595	0.1392907642029759	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	579	0.13554513020760176	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	567	0.13273590471107116	No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	560	0.13109718983809496	Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGTCTGGGCAGCTTTCCCTAGCAGAGGCTCTTTGGCTTCTTTCT	540	0.1264151473438773	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	538	0.12594694309445553	No Hit
ACACCCGGGGAGCGTGGTCACGTGGCCGCTGGTCACCGCCGCCACCTCCCGC	529	0.12384002397205757	No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	507	0.11868977722841811	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	504	0.11798747085428549	No Hit
ACACCCGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT	504	0.11798747085428549	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	502	0.11751926660486371	No Hit
ACACCCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA	488	0.11424183685891133	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGC	469	0.10979389648940455	No Hit
ACACCCGGGACGATGGGTGCCACCCCTCATTGGCGAGATGTATGGGTTGCGC	457	0.10698467099287393	No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT	453	0.10604826249403039	No Hit
ACACCCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	452	0.10581416036931951	No Hit
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC	447	0.1046436497457651	Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	439	0.10277083274807801	No Hit
ACACCCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	432	0.10113211787510182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.009832289237857122	0.0	0.0	0.0	0.0
8	0.025283029468775458	0.0	0.0	0.0	0.0
9	0.025751233718197226	0.0	0.0	0.0	0.0
10	0.04330889307151352	0.0	0.0	0.0	0.0
11	0.06601679916846925	0.0	0.0	0.0	0.0
12	0.08193574364880936	0.0	0.0	0.0	0.0
13	0.1610622618010881	0.0	0.0	0.0	0.0
14	0.22146060997649614	0.0	0.0	0.0	0.0
15	0.26594001367156406	0.0	0.0	0.0	0.0
16	0.3179106853573803	0.0	0.0	0.0	0.0
17	0.3532601061887238	0.0	0.0	0.0	0.0
18	0.38650260789766927	0.0	0.0	0.0	0.0
19	0.4239589478514107	0.0	0.0	0.0	0.0
20	0.44362352632712493	0.0	0.0	0.0	0.0
21	0.5248569636018017	0.0	0.0	0.0	0.0
22	0.5396053974585874	0.0	0.0	0.0	0.0
23	0.5580994653107472	0.0	0.0	0.0	0.0
24	0.5810414735324138	0.0	0.0	0.0	0.0
25	0.5995355413845737	0.0	0.0	0.0	0.0
26	0.6515062130703898	0.0	0.0	0.0	0.0
27	0.6753846297909	0.0	0.0	0.0	0.0
28	0.764109335056325	0.0	0.0	0.0	0.0
29	0.7816669944096413	0.0	0.0	0.0	0.0
30	0.8294238278506616	0.0	0.0	0.0	0.0
31	0.8408948319614948	0.0	0.0	0.0	0.0
32	0.8685188826773792	0.0	0.0	0.0	0.0
33	0.8874811547789607	0.0	0.0	0.0	0.0
34	0.9062093247558315	0.0	0.0	0.0	0.0
35	0.9731625324231443	0.0	0.0	0.0	0.0
36	0.9893155790281952	0.0	0.0	0.0	0.0
37	1.0136621999981272	0.0	0.0	0.0	0.0
38	1.0380088209680591	0.0	0.0	0.0	0.0
39	1.0520549484507122	0.0	0.0	0.0	0.0
40	1.070783118427583	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAACTA	35	1.0175427E-7	46.000004	32
ACACGCG	35	1.0175427E-7	46.000004	12
GCGGTCA	35	1.0175427E-7	46.000004	16
TATATAG	35	1.0175427E-7	46.000004	36
ATATAGG	35	1.0175427E-7	46.000004	37
CGTCGTA	35	1.0175427E-7	46.000004	35
CGGTCAG	35	1.0175427E-7	46.000004	17
CGGTAAC	70	0.0	46.000004	20
GTAACGG	35	1.0175427E-7	46.000004	37
TGCCGAT	35	1.0175427E-7	46.000004	15
ACAACTC	30	1.8586361E-6	46.0	41
TTTAGTA	20	6.3069805E-4	46.0	23
AACGTTC	20	6.3069805E-4	46.0	18
AGACTAC	50	1.6370905E-11	46.0	43
CCTTATA	20	6.3069805E-4	46.0	26
TCGTTTA	25	3.4135264E-5	46.0	28
TCGTTGT	25	3.4135264E-5	46.0	23
ATCTCGT	20	6.3069805E-4	46.0	30
GTCGCAT	20	6.3069805E-4	46.0	38
GCAAGTA	20	6.3069805E-4	46.0	37
>>END_MODULE