SRR1527793_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527793_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429517
Sequences flagged as poor quality	0
Sequence length	52
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1981	0.4612157376774377	No Hit
ACACCCGGGATGTTTCAGGATTCTTTTAACCCTGTTCCAAACTGAGTGTAAT	1810	0.42140357657554883	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG	1707	0.39742315205218887	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1565	0.3643627609617314	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1354	0.3152378136371785	No Hit
ACACCCGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT	1252	0.29149020876938514	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1097	0.2554031621565619	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	929	0.21628946002137284	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	916	0.21326280449900703	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	820	0.1909121175646133	No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	786	0.18299624927534883	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	744	0.17321782374155156	No Hit
ACACCCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	690	0.16064556234095506	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	681	0.15855018544085564	No Hit
ACACCCGGGGTACAGCCTTGAGTGTGAATAATTATTGTAAACTATATTTTAC	631	0.14690920266252558	No Hit
ACACCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	598	0.13922615402882774	No Hit
ACACCCCCCAGTCTGTCCCATCTCAGTTTGCAAAAAGCACTGACACGTATCT	598	0.13922615402882774	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	576	0.1341041216063625	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	540	0.12572261400596482	No Hit
ACACCCGGGGTCTGGGCAGCTTTCCCTAGCAGAGGCTCTTTGGCTTCTTTCT	537	0.12502415503926503	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	536	0.12479133538369842	No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	529	0.12316159779473222	Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA	520	0.1210662208946328	No Hit
ACACCCGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT	493	0.11478009019433455	No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	491	0.11431445088320137	No Hit
ACACCCGGGGAGCGTGGTCACGTGGCCGCTGGTCACCGCCGCCACCTCCCGC	486	0.11315035260536835	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	485	0.11291753294980175	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	482	0.11221907398310195	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	481	0.11198625432753534	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGC	457	0.10639858259393692	No Hit
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC	442	0.10290628776043788	Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGAGGGGGTATGGGGCTGCTGCTGGATCCCGCGCTCCTAGCATTG	441	0.1026734681048713	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAC	70	0.0	46.000004	30
ACCGATT	35	1.01756086E-7	46.000004	36
GATTTGG	35	1.01756086E-7	46.000004	9
TAGCCAC	35	1.01756086E-7	46.000004	40
CCCGTCG	35	1.01756086E-7	46.000004	23
CTTATAC	35	1.01756086E-7	46.000004	42
TCTAATG	35	1.01756086E-7	46.000004	29
CGGGATA	285	0.0	46.000004	6
TTAACGG	35	1.01756086E-7	46.000004	23
GCAATCG	35	1.01756086E-7	46.000004	19
TTCATCG	35	1.01756086E-7	46.000004	33
ATACCGA	35	1.01756086E-7	46.000004	34
TACCGAT	35	1.01756086E-7	46.000004	35
TCACGCG	25	3.413558E-5	46.0	23
TCACGAA	30	1.8586616E-6	46.0	13
CTTAACG	30	1.8586616E-6	46.0	28
TATTTAC	20	6.3070183E-4	46.0	14
AGGTATT	20	6.3070183E-4	46.0	16
TGATATG	65	0.0	46.0	21
TTAGGAT	20	6.3070183E-4	46.0	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content