##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527793_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429517
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03466684671387	31.0	31.0	34.0	31.0	34.0
2	32.48551046873581	34.0	31.0	34.0	31.0	34.0
3	32.470721764214225	34.0	31.0	34.0	31.0	34.0
4	36.22345797721627	37.0	37.0	37.0	35.0	37.0
5	36.16551614953541	37.0	37.0	37.0	35.0	37.0
6	36.2618708921882	37.0	37.0	37.0	35.0	37.0
7	36.363834260343594	37.0	35.0	37.0	35.0	37.0
8	36.508887890351254	37.0	37.0	37.0	35.0	37.0
9	38.578144753292655	39.0	39.0	39.0	37.0	39.0
10	37.84248353382986	39.0	38.0	39.0	35.0	39.0
11	37.65935224915428	39.0	38.0	39.0	35.0	39.0
12	37.17372537059069	39.0	37.0	39.0	34.0	39.0
13	36.89004858946212	39.0	37.0	39.0	33.0	39.0
14	37.8850709052261	40.0	38.0	40.0	33.0	40.0
15	37.94331074206609	40.0	38.0	40.0	33.0	40.0
16	37.899577897964456	40.0	38.0	40.0	33.0	40.0
17	37.96528193296191	40.0	38.0	40.0	34.0	40.0
18	38.000849791742816	40.0	38.0	40.0	34.0	40.0
19	37.7139461301881	40.0	37.0	40.0	33.0	40.0
20	37.78547065657471	40.0	37.0	40.0	33.0	40.0
21	37.8215227802392	40.0	37.0	40.0	34.0	40.0
22	37.69101572231134	40.0	37.0	40.0	33.0	40.0
23	37.689250949322144	40.0	37.0	40.0	33.0	40.0
24	37.659929641900085	40.0	37.0	40.0	33.0	40.0
25	37.522756957233355	40.0	36.0	40.0	33.0	40.0
26	37.32352386517879	40.0	36.0	40.0	33.0	40.0
27	37.18877483312651	40.0	35.0	40.0	33.0	40.0
28	37.071105451006595	39.0	35.0	40.0	32.0	40.0
29	37.01356174493675	39.0	35.0	40.0	32.0	40.0
30	36.78534493396071	39.0	35.0	40.0	31.0	40.0
31	36.664746680573764	39.0	35.0	40.0	31.0	40.0
32	36.38012697984015	39.0	35.0	40.0	31.0	40.0
33	36.09547701255131	38.0	35.0	40.0	30.0	40.0
34	36.01417406063089	38.0	35.0	40.0	30.0	40.0
35	36.00632571004174	38.0	35.0	40.0	30.0	40.0
36	35.760954746843545	38.0	35.0	40.0	30.0	40.0
37	35.59023973439934	38.0	35.0	40.0	29.0	40.0
38	35.24523825599452	37.0	34.0	40.0	28.0	40.0
39	35.266354998754416	37.0	34.0	40.0	28.0	40.0
40	35.142320327251305	37.0	34.0	40.0	28.0	40.0
41	34.776646791628735	36.0	34.0	40.0	27.0	40.0
42	34.80128376758079	36.0	34.0	40.0	27.0	40.0
43	34.752361373356585	36.0	34.0	40.0	28.0	40.0
44	34.523341334568826	35.0	33.0	39.0	27.0	40.0
45	34.47121767008058	35.0	33.0	39.0	27.0	40.0
46	34.12629535035866	35.0	33.0	39.0	26.0	40.0
47	33.80532086040832	35.0	33.0	39.0	25.0	40.0
48	33.774111385579616	35.0	33.0	39.0	25.0	40.0
49	33.70080811702447	35.0	33.0	39.0	26.0	40.0
50	33.38266005769271	35.0	33.0	38.0	24.0	40.0
51	33.63840080834984	35.0	33.0	38.0	26.0	40.0
52	33.39718567600351	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	5.0
16	13.0
17	41.0
18	67.0
19	152.0
20	302.0
21	517.0
22	824.0
23	1362.0
24	1898.0
25	2791.0
26	3900.0
27	4768.0
28	5821.0
29	7146.0
30	8457.0
31	10467.0
32	13469.0
33	18708.0
34	32633.0
35	39852.0
36	56871.0
37	78720.0
38	104019.0
39	36710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	97.61755646458697	1.219974995168992	0.22839608211083612	0.9340724581332054
8	98.28761143330765	0.8982182311759488	0.20185464137624354	0.6123156941401621
9	96.9195631371983	1.736136171560148	0.5543435999040782	0.789957091337479
10	53.63512969218913	32.961675556497184	6.68774460615063	6.7154501451630555
11	37.58407699811649	24.911237506315235	20.40082231902346	17.103863176544817
12	32.0720716525772	15.633607051641729	27.023144601959874	25.2711766938212
13	23.992065506138292	18.70682650512087	27.759320352861472	29.541787635879373
14	26.11514794525013	21.152131347536884	26.78497009431524	25.947750612897742
15	27.22709462023622	19.422281306677036	27.389137100510574	25.961486972576175
16	29.500578556844083	20.69603764228191	25.37897219434854	24.42441160652547
17	32.643178267682075	21.032229224920084	20.75098308099563	25.573609426402218
18	30.83044442944051	20.349136355487676	22.10319964052645	26.71721957454536
19	30.892374457821226	20.9658756230836	22.689206713587588	25.45254320550758
20	29.02469518086595	24.424178786869902	21.734646125764524	24.816479906499627
21	29.230042117075694	19.346149279306758	26.47182765757816	24.951980946039388
22	30.569686415205915	18.601126381493632	22.811437032760054	28.017750170540396
23	26.886479464142283	24.126169627744652	22.802357066192958	26.18499384192011
24	26.32817793009357	24.02279770067308	24.42650698342557	25.22251738580778
25	26.39802382676355	24.066567795919603	22.743453693334605	26.79195468398224
26	28.2582528747407	23.520372883960356	21.288330846043348	26.933043395255602
27	26.46367896963333	20.67624797155875	23.26590100042606	29.594172058381858
28	27.953957584915152	23.660995955922584	22.046857283879334	26.338189175282935
29	30.15689716588633	22.42262820796383	21.85035749458112	25.570117131568715
30	27.68109294859109	22.443581976964825	23.16718546646582	26.708139607978264
31	31.740769282705923	20.979379163106465	22.6382192090185	24.64163234516911
32	30.796685579383354	20.666236726369387	23.98740911302696	24.549668581220303
33	32.205477315216854	21.286235469143246	23.175799793721787	23.332487421918106
34	28.215181238460872	23.632824777599023	23.620252516198427	24.53174146774167
35	27.83824621609855	25.168968865027463	23.85167525383163	23.14110966504236
36	30.112428611673113	23.331323323640273	23.199780218245145	23.35646784644147
37	29.49056731165472	23.41094764584405	23.255889755236698	23.84259528726453
38	31.335430262364472	22.70364153223272	22.379789391339575	23.581138814063237
39	28.74438031556376	22.886870601163633	21.256201733575157	27.11254734969745
40	25.93634244977498	24.990861828519012	23.658434939711352	25.41436078199466
41	27.59890761017608	24.475399111094557	23.035875180726258	24.889818098003104
42	27.09881099001902	20.95563155823867	22.25360113802248	29.69195631371983
43	26.249484886512057	23.935257510180037	25.00925458130877	24.80600302199913
44	26.686720199666137	20.965642803428036	24.125703988433518	28.221933008472305
45	25.804566524724283	22.83914257177248	22.813066770349018	28.543224133154215
46	27.86013126372181	22.623784390373373	22.37047660511691	27.14560774078791
47	27.292982582761567	22.46709676217705	25.11006549216911	25.129855162892273
48	30.204858014933052	22.57116714821532	22.803055525159657	24.42091931169197
49	28.579543999422608	23.361357059208366	23.604420779619897	24.454678161749126
50	26.415019661619915	25.312385772856487	23.581371633718806	24.691222931804795
51	25.979879725365933	24.641399525513542	23.443076758312245	25.935643990808284
52	27.00242365261445	24.147356216401214	24.092876416998628	24.757343713985712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	14.5
8	29.0
9	29.0
10	29.0
11	34.0
12	39.0
13	179.0
14	460.0
15	601.0
16	781.0
17	961.0
18	987.0
19	1013.0
20	740.5
21	468.0
22	609.5
23	751.0
24	758.0
25	765.0
26	928.0
27	1091.0
28	1095.5
29	1100.0
30	1326.5
31	1553.0
32	2131.5
33	2710.0
34	2574.5
35	2439.0
36	3994.0
37	5549.0
38	4884.0
39	4755.0
40	5291.0
41	7156.0
42	9021.0
43	9294.0
44	9567.0
45	11231.0
46	12895.0
47	14731.5
48	16568.0
49	16155.5
50	15743.0
51	17706.5
52	19670.0
53	20561.0
54	21452.0
55	24457.0
56	27462.0
57	29242.5
58	31023.0
59	33603.0
60	36183.0
61	36496.5
62	36810.0
63	36975.0
64	34753.5
65	32367.0
66	31261.5
67	30156.0
68	26663.0
69	23170.0
70	18125.5
71	13081.0
72	11020.0
73	8959.0
74	7836.0
75	6713.0
76	6975.5
77	7238.0
78	5530.0
79	3822.0
80	2330.0
81	838.0
82	670.0
83	502.0
84	283.0
85	64.0
86	100.0
87	136.0
88	71.0
89	3.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	429517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.632908592675026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5397492032288	11.496401772223217
2	10.70205224436303	3.346084089803197
3	4.025556250558485	1.887934586989572
4	2.000119143359247	1.2507071897037836
5	1.0127185535996186	0.7915868289264452
6	0.571888124385667	0.53641648642545
7	0.3574300777410419	0.39113702135189066
8	0.2859440621928335	0.35761099095029997
9	0.21892592261638816	0.30802040431461386
>10	3.498346885890448	15.507651617980198
>50	2.5317963839990467	27.82707087263135
>100	1.2212194322818932	31.31773596854141
>500	0.023828671849402794	2.4751057583285414
>1k	0.010425043934113722	2.5065364118300324
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACACCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1981	0.4612157376774377	No Hit
ACACCCGGGATGTTTCAGGATTCTTTTAACCCTGTTCCAAACTGAGTGTAAT	1810	0.42140357657554883	No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGAAG	1707	0.39742315205218887	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1565	0.3643627609617314	No Hit
ACACCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1354	0.3152378136371785	No Hit
ACACCCGGGCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTTAACAT	1252	0.29149020876938514	No Hit
ACACCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1097	0.2554031621565619	No Hit
ACACCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	929	0.21628946002137284	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	916	0.21326280449900703	No Hit
ACACCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	820	0.1909121175646133	No Hit
ACACCCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	786	0.18299624927534883	No Hit
ACACCCGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	744	0.17321782374155156	No Hit
ACACCCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT	690	0.16064556234095506	No Hit
ACACCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	681	0.15855018544085564	No Hit
ACACCCGGGGTACAGCCTTGAGTGTGAATAATTATTGTAAACTATATTTTAC	631	0.14690920266252558	No Hit
ACACCCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	598	0.13922615402882774	No Hit
ACACCCCCCAGTCTGTCCCATCTCAGTTTGCAAAAAGCACTGACACGTATCT	598	0.13922615402882774	No Hit
ACACCCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA	576	0.1341041216063625	No Hit
ACACCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	540	0.12572261400596482	No Hit
ACACCCGGGGTCTGGGCAGCTTTCCCTAGCAGAGGCTCTTTGGCTTCTTTCT	537	0.12502415503926503	No Hit
ACACCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	536	0.12479133538369842	No Hit
ACACCCGGGTGTAGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	529	0.12316159779473222	Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA	520	0.1210662208946328	No Hit
ACACCCGGGGATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCAGT	493	0.11478009019433455	No Hit
ACACCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	491	0.11431445088320137	No Hit
ACACCCGGGGAGCGTGGTCACGTGGCCGCTGGTCACCGCCGCCACCTCCCGC	486	0.11315035260536835	No Hit
ACACCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	485	0.11291753294980175	No Hit
ACACCCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	482	0.11221907398310195	No Hit
ACACCCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	481	0.11198625432753534	No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCGACTGC	457	0.10639858259393692	No Hit
ACACCCGGGTGTAGCACTGCACGATCGGAAGAGCTCGTATGCCGTCTTCTGC	442	0.10290628776043788	Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGAGGGGGTATGGGGCTGCTGCTGGATCCCGCGCTCCTAGCATTG	441	0.1026734681048713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.00745022897813125	0.0	0.0	0.0	0.0
8	0.021885047623260545	0.0	0.0	0.0	0.0
9	0.024678883490059766	0.0	0.0	0.0	0.0
10	0.04307163627982129	0.0	0.0	0.0	0.0
11	0.06844897873658086	0.0	0.0	0.0	0.0
12	0.08451353497067636	0.0	0.0	0.0	0.0
13	0.15994710337425527	0.0	0.0	0.0	0.0
14	0.21698791898807265	0.0	0.0	0.0	0.0
15	0.267044144934892	0.0	0.0	0.0	0.0
16	0.31710037088171134	0.0	0.0	0.0	0.0
17	0.3557484337057672	0.0	0.0	0.0	0.0
18	0.3871790872072584	0.0	0.0	0.0	0.0
19	0.4255943303757476	0.0	0.0	0.0	0.0
20	0.4467809190323084	0.0	0.0	0.0	0.0
21	0.5229129464025871	0.0	0.0	0.0	0.0
22	0.5368821257365832	0.0	0.0	0.0	0.0
23	0.5580687143931439	0.0	0.0	0.0	0.0
24	0.5832132371943369	0.0	0.0	0.0	0.0
25	0.6011403506729652	0.0	0.0	0.0	0.0
26	0.6509637569642179	0.0	0.0	0.0	0.0
27	0.6747113618320113	0.0	0.0	0.0	0.0
28	0.7606218147360873	0.0	0.0	0.0	0.0
29	0.7766863709701828	0.0	0.0	0.0	0.0
30	0.8202236465611373	0.0	0.0	0.0	0.0
31	0.8320974489950339	0.0	0.0	0.0	0.0
32	0.861898364907559	0.0	0.0	0.0	0.0
33	0.8863444287420521	0.0	0.0	0.0	0.0
34	0.9086951156764459	0.0	0.0	0.0	0.0
35	0.9780753730352931	0.0	0.0	0.0	0.0
36	0.9964681258250546	0.0	0.0	0.0	0.0
37	1.0176547144816155	0.0	0.0	0.0	0.0
38	1.0397725817604426	0.0	0.0	0.0	0.0
39	1.0563027773056712	0.0	0.0	0.0	0.0
40	1.0735314318175997	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAC	70	0.0	46.000004	30
ACCGATT	35	1.01756086E-7	46.000004	36
GATTTGG	35	1.01756086E-7	46.000004	9
TAGCCAC	35	1.01756086E-7	46.000004	40
CCCGTCG	35	1.01756086E-7	46.000004	23
CTTATAC	35	1.01756086E-7	46.000004	42
TCTAATG	35	1.01756086E-7	46.000004	29
CGGGATA	285	0.0	46.000004	6
TTAACGG	35	1.01756086E-7	46.000004	23
GCAATCG	35	1.01756086E-7	46.000004	19
TTCATCG	35	1.01756086E-7	46.000004	33
ATACCGA	35	1.01756086E-7	46.000004	34
TACCGAT	35	1.01756086E-7	46.000004	35
TCACGCG	25	3.413558E-5	46.0	23
TCACGAA	30	1.8586616E-6	46.0	13
CTTAACG	30	1.8586616E-6	46.0	28
TATTTAC	20	6.3070183E-4	46.0	14
AGGTATT	20	6.3070183E-4	46.0	16
TGATATG	65	0.0	46.0	21
TTAGGAT	20	6.3070183E-4	46.0	32
>>END_MODULE