FastQCFastQC Report
Fri 17 Jun 2016
SRR1527792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754672
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT58320.7727860580490598No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC49530.6563116161723239No Hit
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC38650.5121430237242139No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA37750.5002173129518519No Hit
CGGCAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA27660.3665168444039265No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC20260.2684610002756164No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC19650.2603780185299044No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15920.2109525727733373No Hit
CGGCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC15860.21015752538851318No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14300.18948629338308565No Hit
CGGCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG13000.17226026671189604No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12850.17027264824983568No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG12810.1697426166599529No Hit
CGGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA11930.15808192168253227No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA11810.15649182691288402No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11720.1552992558356478No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG11260.1492038925519961No Hit
CGGCAGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT11060.1465537346025823No Hit
CGGCAGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA10490.13900078444675304No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA10390.13767570547204613No Hit
CGGCAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC9630.12760510526427374No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG9280.12296732885279964No Hit
CGGCAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGC9120.12084720249326861No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG9080.12031717090338584No Hit
CGGCAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC8200.10865647592596518No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC8070.10693387325884623No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT7880.10441622320690312No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG7800.10335616002713761No Hit
CGGCAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT7740.10256111264231348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG207.854761E-444.042
ATTTACG302.526558E-644.036
GATAATC207.854761E-444.010
CATATCA207.854761E-444.020
CGACGTA207.854761E-444.034
TTCGTTA207.854761E-444.041
ATTCGTT302.526558E-644.033
TTGTACG254.441382E-544.039
CGATTGG254.441382E-544.023
TAACGTC454.802132E-1044.024
CGATTGA207.854761E-444.035
CACGACC302.526558E-644.013
TATGCGG207.854761E-444.019
TTATCGA302.526558E-644.014
ATCGTTT207.854761E-444.031
CGTTAGT207.854761E-444.043
AAACGAC207.854761E-444.036
CCGTAAC254.441382E-544.043
TTAAGCG207.854761E-444.023
ACAATCG207.854761E-444.028
Produced by FastQC (version 0.11.5)