##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527792_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	754672
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45043542095109	33.0	31.0	34.0	31.0	34.0
2	32.83593269658871	34.0	31.0	34.0	31.0	34.0
3	33.116775234803995	34.0	33.0	34.0	31.0	34.0
4	36.39179140076749	37.0	37.0	37.0	35.0	37.0
5	36.25154504208451	37.0	37.0	37.0	35.0	37.0
6	36.354143256938116	37.0	37.0	37.0	35.0	37.0
7	36.586160610172364	37.0	37.0	37.0	35.0	37.0
8	36.635835170776176	37.0	37.0	37.0	35.0	37.0
9	38.650120847202494	39.0	39.0	39.0	38.0	39.0
10	38.037783301884794	39.0	38.0	39.0	37.0	39.0
11	36.729095554095025	38.0	35.0	39.0	33.0	39.0
12	36.47073430576462	39.0	35.0	39.0	33.0	39.0
13	36.28147327580724	38.0	35.0	39.0	32.0	39.0
14	36.95435235440032	39.0	35.0	40.0	33.0	40.0
15	37.09165836283842	39.0	35.0	40.0	33.0	40.0
16	37.18164447601077	39.0	35.0	40.0	33.0	40.0
17	37.115038056268155	39.0	35.0	40.0	33.0	40.0
18	37.034312920050034	39.0	35.0	40.0	33.0	40.0
19	36.98430178938665	39.0	35.0	40.0	32.0	40.0
20	36.79417415777981	39.0	35.0	40.0	31.0	40.0
21	36.72694627599805	38.0	35.0	40.0	31.0	40.0
22	36.7094247567155	38.0	35.0	40.0	32.0	40.0
23	36.422277492738566	38.0	35.0	40.0	31.0	40.0
24	36.21374981448894	38.0	35.0	40.0	31.0	40.0
25	36.16918608349057	38.0	35.0	40.0	31.0	40.0
26	36.01165804481947	38.0	35.0	40.0	31.0	40.0
27	35.90854172408675	38.0	35.0	40.0	30.0	40.0
28	35.6994654631416	38.0	34.0	40.0	30.0	40.0
29	36.02989245659041	38.0	35.0	40.0	30.0	40.0
30	36.22739680284945	38.0	35.0	40.0	31.0	40.0
31	36.249550798227574	38.0	35.0	40.0	31.0	40.0
32	36.21324495939958	38.0	35.0	40.0	31.0	40.0
33	36.09718791739988	38.0	35.0	40.0	31.0	40.0
34	35.68257203129306	38.0	35.0	40.0	30.0	40.0
35	35.61887415989993	38.0	34.0	40.0	29.0	40.0
36	35.560479254563575	37.0	34.0	40.0	29.0	40.0
37	35.36660827485318	37.0	34.0	40.0	29.0	40.0
38	35.203516229567285	37.0	34.0	40.0	28.0	40.0
39	35.027266150062545	36.0	34.0	40.0	28.0	40.0
40	34.79058981915322	36.0	34.0	40.0	27.0	40.0
41	34.64997773867322	36.0	34.0	40.0	27.0	40.0
42	34.49785867237687	36.0	34.0	40.0	26.0	40.0
43	34.42881278224182	35.0	33.0	40.0	26.0	40.0
44	34.09835398689762	35.0	33.0	40.0	24.0	40.0
45	34.04943207115144	35.0	33.0	39.0	25.0	40.0
46	33.962313428880364	35.0	33.0	39.0	24.0	40.0
47	33.77132317086098	35.0	33.0	39.0	23.0	40.0
48	33.660734729789894	35.0	33.0	39.0	23.0	40.0
49	33.570553830008265	35.0	33.0	39.0	24.0	40.0
50	32.97323737994785	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	3.0
14	14.0
15	34.0
16	54.0
17	149.0
18	275.0
19	546.0
20	842.0
21	1337.0
22	1989.0
23	2689.0
24	3812.0
25	5169.0
26	6619.0
27	9138.0
28	12001.0
29	15063.0
30	17918.0
31	22046.0
32	28674.0
33	42329.0
34	82009.0
35	69007.0
36	80957.0
37	112182.0
38	191196.0
39	48618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.71692602879132	1.049065024275447	0.16470731655606674	0.06930163037717048
8	98.53671528823118	1.0840471092077089	0.2393092628320648	0.13992833972904786
9	95.9579790955541	1.660058939512795	1.2378887781711791	1.1440731867619311
10	56.704237072529516	29.523687111751862	6.523893824071915	7.248181991646701
11	35.43658702058642	24.87650263955732	20.765047596836773	18.921862743019485
12	31.46373523862022	17.591615960311234	26.917654292195813	24.02699450887273
13	25.220625649288696	16.844536434371488	28.42718426018191	29.507653656157906
14	26.18594568236267	19.463820043674605	29.339898657960013	25.010335616002717
15	26.873661670235542	19.541469671592427	28.36066529565162	25.22420336252041
16	30.962457862488602	20.575031271863804	23.206770623529163	25.255740242118428
17	30.695851972777575	23.190737167935207	21.918661352216592	24.19474950707062
18	30.754817987152034	20.746231475395934	22.190170034134034	26.308780503317998
19	31.707019738376406	22.363490364025694	21.1446827230903	24.784807174507602
20	31.98078105455085	21.73672800898934	22.337783831916383	23.94470710454343
21	32.37485954162868	20.28033370788899	23.801466067377618	23.543340683104713
22	31.08078741492993	19.48859902050162	24.337858036338964	25.092755528229482
23	29.461010876248224	21.6971081476456	24.24059723959548	24.601283736510695
24	28.568702694680603	22.887559098522274	23.646961858926794	24.896776347870333
25	29.90212966692815	22.57749061844086	21.296006742001826	26.22437297262917
26	27.23183051709882	23.363924989929398	21.340661903449444	28.063582589522333
27	27.10886318824602	21.750243814531345	23.339543536794793	27.801349460427843
28	28.386769351453346	22.406952954396083	22.00810418300931	27.198173511141267
29	29.964143362944434	23.198025102296096	22.557746941717728	24.280084593041746
30	29.270729535480317	21.207756482286342	22.38482413551848	27.136689846714862
31	28.77780015688935	22.542376025611127	22.581863379057392	26.09796043844213
32	32.50127207581572	22.325460597451606	21.914156083702586	23.259111243030084
33	32.82260372718214	22.546351262535246	21.568575487099032	23.06246952318358
34	28.866182924502297	23.922710793563297	24.978401212712278	22.232705069222124
35	28.206426102995742	25.98082345707804	23.68737676765535	22.125373672270868
36	29.854426823838704	25.004372760616533	21.56817796340662	23.573022452138147
37	30.041660482964783	26.269955689359087	20.407408781563383	23.280975046112747
38	31.095363283651707	24.764930989886995	21.890172154260394	22.249533572200903
39	29.192814891767547	23.859239510674836	21.930189539296542	25.01775605826107
40	29.093964000254413	23.1641030805436	22.74723323510081	24.994699684101175
41	25.99553183369729	23.651732143235737	23.656767443339625	26.695968579727353
42	26.638592660122544	22.830050671019993	24.27133907180868	26.260017597048783
43	27.711244090147773	20.601267835563	25.999242054826468	25.688246019462763
44	27.539646362923232	20.947908495346322	24.83330506498187	26.679140076748574
45	26.93779549261136	21.29375410774482	24.352168889265798	27.41628151037802
46	27.224940106430346	23.02390972501961	23.626820658511246	26.124329510038795
47	25.731443594038204	24.85185617062777	24.194616999173153	25.222083236160874
48	28.128776475077917	23.6197977399453	23.937154154387603	24.314271630589182
49	27.41058367078678	22.740342824432336	23.93092628320648	25.918147221574404
50	24.83065490703246	24.568421777937964	24.024476858820787	26.576446456208792
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.5
10	13.0
11	12.5
12	12.0
13	97.0
14	182.0
15	593.5
16	1005.0
17	1488.0
18	1971.0
19	2625.0
20	3279.0
21	2527.5
22	1776.0
23	1992.0
24	2208.0
25	2190.0
26	2172.0
27	2278.0
28	2384.0
29	2498.0
30	2612.0
31	3119.0
32	3626.0
33	3704.0
34	3782.0
35	5168.5
36	6555.0
37	7211.5
38	7868.0
39	9409.5
40	10951.0
41	11020.0
42	11089.0
43	12793.5
44	14498.0
45	17422.5
46	20347.0
47	20043.5
48	19740.0
49	21638.5
50	23537.0
51	24585.0
52	25633.0
53	27609.5
54	29586.0
55	32521.0
56	35456.0
57	38720.5
58	41985.0
59	44034.5
60	46084.0
61	56667.0
62	67250.0
63	72294.0
64	77338.0
65	75328.0
66	73318.0
67	66171.0
68	59024.0
69	54271.5
70	49519.0
71	42088.0
72	34657.0
73	29606.0
74	24555.0
75	20929.0
76	17303.0
77	14085.0
78	10867.0
79	12266.0
80	13665.0
81	9720.0
82	5775.0
83	3834.5
84	1894.0
85	1359.0
86	824.0
87	521.5
88	219.0
89	134.0
90	49.0
91	40.0
92	31.0
93	25.5
94	20.0
95	14.0
96	8.0
97	6.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	754672.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.588345665401658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.89098268804172	8.215092119490322
2	8.852653966656757	2.0517522844361524
3	3.1902485878290303	1.109091101829669
4	1.5951242939145152	0.739394067886446
5	0.8793194136345964	0.5094928657748002
6	0.5980286779335422	0.4158097822630229
7	0.44366181078052463	0.35989144953039204
8	0.378484689093695	0.3508809125023851
9	0.3178814005076955	0.3315347594716645
>10	8.339241201088573	27.64777280725931
>50	3.2085439202323505	25.378045031483875
>100	1.2452260617010085	24.49434986325185
>500	0.037734123081848744	2.894900036042148
>1k	0.021725707228943215	4.729206860728899
>5k	0.0011434582752075377	0.7727860580490598
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5832	0.7727860580490598	No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4953	0.6563116161723239	No Hit
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3865	0.5121430237242139	No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	3775	0.5002173129518519	No Hit
CGGCAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	2766	0.3665168444039265	No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2026	0.2684610002756164	No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1965	0.2603780185299044	No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1592	0.2109525727733373	No Hit
CGGCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	1586	0.21015752538851318	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1430	0.18948629338308565	No Hit
CGGCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1300	0.17226026671189604	No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1285	0.17027264824983568	No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1281	0.1697426166599529	No Hit
CGGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	1193	0.15808192168253227	No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1181	0.15649182691288402	No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1172	0.1552992558356478	No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1126	0.1492038925519961	No Hit
CGGCAGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT	1106	0.1465537346025823	No Hit
CGGCAGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA	1049	0.13900078444675304	No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	1039	0.13767570547204613	No Hit
CGGCAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	963	0.12760510526427374	No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	928	0.12296732885279964	No Hit
CGGCAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGC	912	0.12084720249326861	No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	908	0.12031717090338584	No Hit
CGGCAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	820	0.10865647592596518	No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	807	0.10693387325884623	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	788	0.10441622320690312	No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	780	0.10335616002713761	No Hit
CGGCAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	774	0.10256111264231348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0837449912014756	0.0	0.0	0.0	0.0
6	0.0837449912014756	0.0	0.0	0.0	0.0
7	0.08639514915088939	0.0	0.0	0.0	0.0
8	0.09885089151313418	0.0	0.0	0.0	0.0
9	0.10295863633472555	0.0	0.0	0.0	0.0
10	0.13873576865181164	0.0	0.0	0.0	0.0
11	0.1627196980940064	0.0	0.0	0.0	0.0
12	0.1786206457904891	0.0	0.0	0.0	0.0
13	0.19187143553755803	0.0	0.0	0.0	0.0
14	0.21294019123539762	0.0	0.0	0.0	0.0
15	0.22910615472682172	0.0	0.0	0.0	0.0
16	0.24739224457777684	0.0	0.0	0.0	0.0
17	0.26448576335149576	0.0	0.0	0.0	0.0
18	0.27826658468844745	0.0	0.0	0.0	0.0
19	0.2997328640786991	0.0	0.0	0.0	0.0
20	0.3143087328004749	0.0	0.0	0.0	0.0
21	0.3341849174210783	0.0	0.0	0.0	0.0
22	0.3508809125023851	0.0	0.0	0.0	0.0
23	0.37314223927746093	0.0	0.0	0.0	0.0
24	0.3939459791803592	0.0	0.0	0.0	0.0
25	0.4102444505692539	0.0	0.0	0.0	0.0
26	0.4287955562151504	0.0	0.0	0.0	0.0
27	0.4464191065787521	0.0	0.0	0.0	0.0
28	0.47278817817541924	0.0	0.0	0.0	0.0
29	0.48749655479466575	0.0	0.0	0.0	0.0
30	0.5052526130557381	0.0	0.0	0.0	0.0
31	0.5228761634193398	0.0	0.0	0.0	0.0
32	0.5434148875272966	0.0	0.0	0.0	0.0
33	0.5582557720440138	0.0	0.0	0.0	0.0
34	0.5765418618949689	0.0	0.0	0.0	0.0
35	0.5999957597472809	0.0	0.0	0.0	0.0
36	0.6162942311361758	0.0	0.0	0.0	0.0
37	0.6323276867301291	0.0	0.0	0.0	0.0
38	0.6458434922721394	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	7.854761E-4	44.0	42
ATTTACG	30	2.526558E-6	44.0	36
GATAATC	20	7.854761E-4	44.0	10
CATATCA	20	7.854761E-4	44.0	20
CGACGTA	20	7.854761E-4	44.0	34
TTCGTTA	20	7.854761E-4	44.0	41
ATTCGTT	30	2.526558E-6	44.0	33
TTGTACG	25	4.441382E-5	44.0	39
CGATTGG	25	4.441382E-5	44.0	23
TAACGTC	45	4.802132E-10	44.0	24
CGATTGA	20	7.854761E-4	44.0	35
CACGACC	30	2.526558E-6	44.0	13
TATGCGG	20	7.854761E-4	44.0	19
TTATCGA	30	2.526558E-6	44.0	14
ATCGTTT	20	7.854761E-4	44.0	31
CGTTAGT	20	7.854761E-4	44.0	43
AAACGAC	20	7.854761E-4	44.0	36
CCGTAAC	25	4.441382E-5	44.0	43
TTAAGCG	20	7.854761E-4	44.0	23
ACAATCG	20	7.854761E-4	44.0	28
>>END_MODULE