FastQCFastQC Report
Fri 17 Jun 2016
SRR1527791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150864
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG93640.8136495711048395No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA80200.6968677445814623No Hit
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT59410.5162208566781131No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA58280.5064021465611923No Hit
CGGCAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG41510.360685537126889No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC33400.290216741508988No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC29880.2596310250385797No Hit
CGGCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG24290.21105882189381195No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT23610.2051502175756649No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC20860.1812551265831584No Hit
CGGCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT20270.17612854342476608No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG20040.17413004490539283No Hit
CGGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG18020.15657801443089714No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC17200.14945293275313154No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17170.14919225903321331No Hit
CGGCAGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG16980.14754132547373103No Hit
CGGCAGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA16870.14658552183403076No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT16750.14554282695435777No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15660.13607168179732793No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC15210.13216157599855413No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG14620.12703499284016181No Hit
CGGCAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT13840.12025747612228727No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13450.11686871776334998No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT13100.1138275243643037No Hit
CGGCAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTC12850.11165524336498492No Hit
CGGCAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT12600.10948296236566613No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG11710.1017496420080913No Hit
CGGCAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA11650.10122829456825481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATGT700.046.00000428
TAATCGG351.01957994E-746.00000431
ACACGCT253.4171593E-546.040
AATTTCG453.092282E-1046.011
CATATCG301.8614064E-646.046
CGTATAT206.311458E-446.036
CGACGTA253.4171593E-546.034
CCGATTC253.4171593E-546.046
TAGCGTC453.092282E-1046.045
ATTCGGC206.311458E-446.016
ATTCGAG301.8614064E-646.010
CACGATC206.311458E-446.040
ATCCGAT206.311458E-446.046
CCCCGTA301.8614064E-646.026
CCGTAGA453.092282E-1046.019
TACGCCT206.311458E-446.025
TACGCCC253.4171593E-546.030
TATTCGA206.311458E-446.034
ACAATCG253.4171593E-546.028
TCGTAAG206.311458E-446.043
Produced by FastQC (version 0.11.5)