##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527791_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1150864
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23029046003698	33.0	31.0	34.0	31.0	34.0
2	32.549243872429756	34.0	31.0	34.0	31.0	34.0
3	32.85558067677849	34.0	31.0	34.0	31.0	34.0
4	36.242978318897805	37.0	37.0	37.0	35.0	37.0
5	35.98830096345007	37.0	35.0	37.0	35.0	37.0
6	36.11202453113487	37.0	35.0	37.0	35.0	37.0
7	36.547189763516805	37.0	37.0	37.0	35.0	37.0
8	36.60194688512283	37.0	37.0	37.0	35.0	37.0
9	38.609046768341	39.0	39.0	39.0	37.0	39.0
10	37.88646269237721	39.0	38.0	39.0	35.0	39.0
11	36.58979688303744	38.0	35.0	39.0	33.0	39.0
12	36.342866750545674	39.0	35.0	39.0	32.0	39.0
13	36.22559051286685	38.0	35.0	39.0	32.0	39.0
14	36.94522463123358	39.0	35.0	40.0	32.0	40.0
15	37.096543118908926	39.0	35.0	40.0	33.0	40.0
16	37.20321688748627	39.0	35.0	40.0	33.0	40.0
17	37.17414481641619	39.0	35.0	40.0	33.0	40.0
18	37.158564348176675	39.0	35.0	40.0	33.0	40.0
19	37.13019609615037	39.0	35.0	40.0	33.0	40.0
20	37.04155486660457	39.0	35.0	40.0	32.0	40.0
21	36.981473918725406	39.0	35.0	40.0	32.0	40.0
22	36.936281784815584	39.0	35.0	40.0	32.0	40.0
23	36.734667171794406	39.0	35.0	40.0	32.0	40.0
24	36.57849493945418	38.0	35.0	40.0	31.0	40.0
25	36.548943228739454	38.0	35.0	40.0	31.0	40.0
26	36.41395073614259	38.0	35.0	40.0	31.0	40.0
27	36.34068578042236	38.0	35.0	40.0	31.0	40.0
28	36.21584218465431	38.0	35.0	40.0	31.0	40.0
29	36.08223647624741	38.0	35.0	40.0	31.0	40.0
30	35.938473181887694	38.0	35.0	40.0	30.0	40.0
31	35.75075334705056	38.0	34.0	40.0	30.0	40.0
32	35.61710419302368	38.0	34.0	40.0	30.0	40.0
33	35.43892849198515	37.0	34.0	40.0	29.0	40.0
34	35.195809409278596	37.0	34.0	40.0	28.0	40.0
35	35.032640694295765	37.0	33.0	40.0	28.0	40.0
36	34.994565821852106	36.0	33.0	40.0	28.0	40.0
37	34.839363295749976	36.0	33.0	40.0	28.0	40.0
38	34.66028305690334	36.0	33.0	40.0	27.0	40.0
39	34.51643895369045	36.0	33.0	40.0	27.0	40.0
40	34.2407591166289	35.0	33.0	39.0	26.0	40.0
41	34.070712960002226	35.0	33.0	39.0	25.0	40.0
42	33.93403564626229	35.0	33.0	39.0	25.0	40.0
43	33.82179823158948	35.0	33.0	39.0	25.0	40.0
44	33.662361495363484	35.0	33.0	39.0	24.0	40.0
45	33.542431599215895	35.0	32.0	38.0	24.0	40.0
46	33.43102920935923	35.0	32.0	38.0	24.0	40.0
47	33.16792166580934	35.0	32.0	38.0	23.0	40.0
48	32.93441362315617	35.0	32.0	38.0	23.0	40.0
49	33.390710805099474	35.0	32.0	38.0	24.0	40.0
50	33.55170897690778	35.0	33.0	38.0	26.0	40.0
51	33.53546379068248	35.0	33.0	38.0	26.0	40.0
52	33.1007503927484	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	16.0
15	26.0
16	80.0
17	195.0
18	475.0
19	815.0
20	1476.0
21	2208.0
22	3390.0
23	4841.0
24	6769.0
25	8819.0
26	11678.0
27	15303.0
28	18650.0
29	23671.0
30	29680.0
31	38697.0
32	49747.0
33	72118.0
34	130273.0
35	110332.0
36	131856.0
37	176193.0
38	251312.0
39	62241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.89092021298781	0.9252179232298516	0.1339862920379819	0.049875571744359026
8	98.6428457228656	1.0292267374772344	0.21792322985165927	0.11000430980550266
9	96.08016238234927	1.6619687469588067	1.1677313739937993	1.0901374966981328
10	56.63753493027846	29.555099473091524	6.390155570076048	7.417210026553962
11	35.25055957958542	24.89008258143447	20.873187448734168	18.986170390245938
12	31.402407234912204	17.52570242878394	26.930028222274743	24.14186211402911
13	25.424029251067026	16.685377247007466	28.319245366959084	29.571348134966424
14	26.380267346967145	19.330520374257947	29.17434205953093	25.11487021924398
15	26.96965062770232	19.3883899400798	28.186910008480588	25.455049423737297
16	30.83518122037009	20.549256906115755	23.28033546969929	25.335226403814875
17	30.782698911426547	23.102990448914902	21.97018935339015	24.1441212862684
18	31.042590610185044	20.54499923535709	22.160133603970582	26.252276550487284
19	31.796980355628467	22.178120090644942	21.196335970192827	24.828563583533764
20	32.18555798078661	21.536080718486286	22.168214489288047	24.110146811439055
21	32.52147951452126	20.216811021980007	23.869371185474563	23.392338278024162
22	31.086557577611256	19.37605138400367	24.369864727717612	25.167526310667466
23	29.4043431717388	21.66667825216533	24.313732986695214	24.615245589400658
24	28.42551335344576	22.71667199599605	23.88501160866966	24.97280304188853
25	29.894757330145005	22.408729441532625	21.457009690111082	26.239503538211288
26	27.091385254904143	23.130882536946153	21.56753534735642	28.21019686079328
27	26.944886624310083	21.723070666907645	23.44847001904656	27.883572689735715
28	28.32576220995704	22.22382488287061	22.013113625936686	27.43729928123566
29	29.912830708059335	22.993594377789208	22.62474106410488	24.468833850046572
30	29.39591472146144	21.057918224916236	22.25197764462178	27.29418940900054
31	28.920706530050467	22.402560163494556	22.613445202908427	26.06328810354655
32	32.517134952522625	22.21565710631317	21.956373646234482	23.310834294929723
33	32.914141027958124	22.376405900262757	21.42625019116073	23.283202880618386
34	28.78837117157197	23.697500312808465	25.094016321650514	22.420112193969054
35	28.24391066190271	25.69017711910356	23.783696422861432	22.282215796132295
36	29.792747014416992	24.812836269098696	21.611328532302686	23.783088184181622
37	29.949151246367943	26.254275049006658	20.501727397850658	23.294846306774737
38	31.13443465083624	24.586658371449623	22.00503274061922	22.273874237094912
39	29.156790029056427	23.678036675054567	21.914926524767477	25.25024677112152
40	29.014896634180932	23.063889390927166	22.886978826342645	25.034235148549268
41	25.9882140722101	23.486441490914654	23.67586439405525	26.84948004282
42	26.570646053747442	22.609187532149758	24.349792851283905	26.47037356281889
43	27.70448984415187	20.375561317410224	26.114814608850395	25.805134229587512
44	27.42383113903989	20.83599799802583	24.92353570882398	26.8166351541103
45	26.944017711910355	21.20554644162994	24.32980786609017	27.520627980369532
46	27.067750837631554	22.730313920671776	23.82922743260715	26.37270780908952
47	25.9107070861544	24.514712424752187	24.218326405205133	25.35625408388828
48	28.30847085320246	23.432482030891574	23.925937382696826	24.333109733209138
49	27.394461899929095	22.637253402660956	24.038374647221566	25.929910050188383
50	24.741237887361148	24.434164245297445	24.27767312210652	26.546924745234886
51	25.631786205841873	23.532754521820127	23.720874056361133	27.114585215976867
52	27.85350832070514	23.317785594127542	24.09945918892241	24.72924689624491
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	17.5
10	35.0
11	23.0
12	11.0
13	145.0
14	868.5
15	1458.0
16	2096.5
17	2735.0
18	3557.0
19	4379.0
20	3476.5
21	2574.0
22	2717.5
23	2861.0
24	2752.5
25	2644.0
26	2639.0
27	2634.0
28	2976.5
29	3319.0
30	3623.0
31	3927.0
32	4703.0
33	5479.0
34	6441.5
35	7404.0
36	8251.5
37	9099.0
38	11065.0
39	15586.0
40	18141.0
41	18560.5
42	18980.0
43	20578.0
44	22176.0
45	25512.0
46	28848.0
47	29307.0
48	29766.0
49	32197.0
50	34628.0
51	36988.0
52	39348.0
53	40612.5
54	41877.0
55	46877.0
56	51877.0
57	57596.5
58	63316.0
59	66354.0
60	69392.0
61	81252.5
62	93113.0
63	104467.5
64	110515.5
65	105209.0
66	99681.0
67	94153.0
68	84485.0
69	74817.0
70	67572.0
71	60327.0
72	49691.5
73	39056.0
74	33772.0
75	28488.0
76	24785.0
77	21082.0
78	20540.5
79	19999.0
80	16184.0
81	12369.0
82	7932.5
83	3496.0
84	2617.5
85	1739.0
86	1228.0
87	717.0
88	434.5
89	99.0
90	46.0
91	37.0
92	28.0
93	19.0
94	10.0
95	11.0
96	12.0
97	11.5
98	11.0
99	5.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1150864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.597438643618124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.83883136854706	6.798713376452414
2	9.178402990590383	1.761783190971846
3	3.93068699519345	1.1317358179091048
4	2.0643107421684155	0.7924838275729265
5	1.1129392660527209	0.5340683160007189
6	0.6393073875853527	0.36814280560573287
7	0.45638740546053624	0.3066105085139295
8	0.36130648812486554	0.277409348105563
9	0.29248618262957005	0.25264063727040453
>10	4.93785490986073	13.468431261315263
>50	3.8910622677436475	26.716725360908107
>100	2.2013433495193873	36.38129424583495
>500	0.06610368758326421	4.381624652410923
>1k	0.025354839073032844	4.294716969438693
>5k	0.0036221198675761204	2.533619681689423
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9364	0.8136495711048395	No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8020	0.6968677445814623	No Hit
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5941	0.5162208566781131	No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	5828	0.5064021465611923	No Hit
CGGCAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	4151	0.360685537126889	No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3340	0.290216741508988	No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2988	0.2596310250385797	No Hit
CGGCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	2429	0.21105882189381195	No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2361	0.2051502175756649	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2086	0.1812551265831584	No Hit
CGGCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2027	0.17612854342476608	No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2004	0.17413004490539283	No Hit
CGGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1802	0.15657801443089714	No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1720	0.14945293275313154	No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1717	0.14919225903321331	No Hit
CGGCAGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1698	0.14754132547373103	No Hit
CGGCAGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA	1687	0.14658552183403076	No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1675	0.14554282695435777	No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1566	0.13607168179732793	No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1521	0.13216157599855413	No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1462	0.12703499284016181	No Hit
CGGCAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1384	0.12025747612228727	No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1345	0.11686871776334998	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	1310	0.1138275243643037	No Hit
CGGCAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTC	1285	0.11165524336498492	No Hit
CGGCAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1260	0.10948296236566613	No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1171	0.1017496420080913	No Hit
CGGCAGGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGA	1165	0.10122829456825481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.06994744817806448	0.0	0.0	0.0	0.0
6	0.06994744817806448	0.0	0.0	0.0	0.0
7	0.07238040289730151	0.0	0.0	0.0	0.0
8	0.08376315533373188	0.0	0.0	0.0	0.0
9	0.08880284725215143	0.0	0.0	0.0	0.0
10	0.12147395348190577	0.0	0.0	0.0	0.0
11	0.14154582991561124	0.0	0.0	0.0	0.0
12	0.15353682103185085	0.0	0.0	0.0	0.0
13	0.166049159587927	0.0	0.0	0.0	0.0
14	0.18290606014264066	0.0	0.0	0.0	0.0
15	0.19828580961781758	0.0	0.0	0.0	0.0
16	0.21383934157294	0.0	0.0	0.0	0.0
17	0.22913219980814414	0.0	0.0	0.0	0.0
18	0.24260034200392053	0.0	0.0	0.0	0.0
19	0.262324653477735	0.0	0.0	0.0	0.0
20	0.2770092730331299	0.0	0.0	0.0	0.0
21	0.2923890225083068	0.0	0.0	0.0	0.0
22	0.3081163369433747	0.0	0.0	0.0	0.0
23	0.32749308345729816	0.0	0.0	0.0	0.0
24	0.3456533526116031	0.0	0.0	0.0	0.0
25	0.3594690597672705	0.0	0.0	0.0	0.0
26	0.37676041652184794	0.0	0.0	0.0	0.0
27	0.39144503607724285	0.0	0.0	0.0	0.0
28	0.41090867383113905	0.0	0.0	0.0	0.0
29	0.4234210123872152	0.0	0.0	0.0	0.0
30	0.4370629370629371	0.0	0.0	0.0	0.0
31	0.4511393179385227	0.0	0.0	0.0	0.0
32	0.46730108857345437	0.0	0.0	0.0	0.0
33	0.4807692307692308	0.0	0.0	0.0	0.0
34	0.49536695908465295	0.0	0.0	0.0	0.0
35	0.5162208566781131	0.0	0.0	0.0	0.0
36	0.5306448025135898	0.0	0.0	0.0	0.0
37	0.5453294220689847	0.0	0.0	0.0	0.0
38	0.5583631080648973	0.0	0.0	0.0	0.0
39	0.5702672079411643	0.0	0.0	0.0	0.0
40	0.5833008939370768	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATGT	70	0.0	46.000004	28
TAATCGG	35	1.01957994E-7	46.000004	31
ACACGCT	25	3.4171593E-5	46.0	40
AATTTCG	45	3.092282E-10	46.0	11
CATATCG	30	1.8614064E-6	46.0	46
CGTATAT	20	6.311458E-4	46.0	36
CGACGTA	25	3.4171593E-5	46.0	34
CCGATTC	25	3.4171593E-5	46.0	46
TAGCGTC	45	3.092282E-10	46.0	45
ATTCGGC	20	6.311458E-4	46.0	16
ATTCGAG	30	1.8614064E-6	46.0	10
CACGATC	20	6.311458E-4	46.0	40
ATCCGAT	20	6.311458E-4	46.0	46
CCCCGTA	30	1.8614064E-6	46.0	26
CCGTAGA	45	3.092282E-10	46.0	19
TACGCCT	20	6.311458E-4	46.0	25
TACGCCC	25	3.4171593E-5	46.0	30
TATTCGA	20	6.311458E-4	46.0	34
ACAATCG	25	3.4171593E-5	46.0	28
TCGTAAG	20	6.311458E-4	46.0	43
>>END_MODULE