FastQCFastQC Report
Fri 17 Jun 2016
SRR1527790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163420
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG90160.7749565934916024No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA78250.6725859964587165No Hit
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT55260.474978941396916No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA55000.4727441508655516No Hit
CGGCAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG40740.35017448556841035No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC32980.2834745835553798No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC27930.24006807515772463No Hit
CGGCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG23160.19906826425538496No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT21650.1860892884770762No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19800.17018789431159856No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG19300.16589022021282082No Hit
CGGCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT18940.16279589486170085No Hit
CGGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG17450.14998882604734318No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16680.14337040793522546No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16590.14259682659744546No Hit
CGGCAGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA16490.1417372917776899No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT16000.13752557116088773No Hit
CGGCAGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG14790.1271251998418456No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14680.12617971154011448No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC14370.12351515359887229No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG13940.11981915387392343No Hit
CGGCAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT13620.1170686424507057No Hit
CGGCAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTC12860.11053617782056352No Hit
CGGCAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT12720.10933282907290573No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC12550.10787161987932131No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT12090.10391775970844579No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG11900.10228464355091026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA206.3114864E-446.032
AGCGTAA206.3114864E-446.025
CATATCG206.3114864E-446.046
CGAAAGG206.3114864E-446.030
TAGGTCG253.4171826E-546.026
CCGCTTA206.3114864E-446.011
TTATCGG253.4171826E-546.017
TCGATAT206.3114864E-446.042
GTAGACG253.4171826E-546.010
TACGACA206.3114864E-446.033
CACTAAT253.4171826E-546.030
GTCGTAA253.4171826E-546.015
TTAACGG405.6115823E-946.013
CGATGAT301.8614228E-646.030
CGCAAAC253.4171826E-546.035
AATACGA206.3114864E-446.034
ATATTCG206.3114864E-446.043
TTGTACG351.01957994E-745.99999639
TACGTTC351.01957994E-745.99999635
TGCGTCG2350.045.0212839
Produced by FastQC (version 0.11.5)