##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527790_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1163420
Sequences flagged as poor quality	0
Sequence length	52
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17525571160888	33.0	31.0	34.0	31.0	34.0
2	32.508899623523746	34.0	31.0	34.0	31.0	34.0
3	32.82974592150728	34.0	31.0	34.0	31.0	34.0
4	36.227057296591084	37.0	37.0	37.0	35.0	37.0
5	36.009972322978804	37.0	35.0	37.0	35.0	37.0
6	36.010531880146466	37.0	35.0	37.0	35.0	37.0
7	36.49747468669956	37.0	37.0	37.0	35.0	37.0
8	36.5683562256107	37.0	37.0	37.0	35.0	37.0
9	38.564741022158806	39.0	39.0	39.0	37.0	39.0
10	37.800062746041846	39.0	38.0	39.0	35.0	39.0
11	36.55541249076	38.0	35.0	39.0	33.0	39.0
12	36.29194100152997	39.0	35.0	39.0	32.0	39.0
13	36.02943391036771	38.0	35.0	39.0	31.0	39.0
14	36.863588385965514	39.0	35.0	40.0	32.0	40.0
15	37.03745680837531	39.0	35.0	40.0	32.0	40.0
16	37.176518368259096	39.0	35.0	40.0	33.0	40.0
17	37.17876948995204	39.0	35.0	40.0	33.0	40.0
18	37.17198174348043	39.0	35.0	40.0	33.0	40.0
19	37.09723745508931	39.0	35.0	40.0	33.0	40.0
20	37.028491000670435	39.0	35.0	40.0	32.0	40.0
21	37.0265836929054	39.0	35.0	40.0	32.0	40.0
22	36.87517319626618	39.0	35.0	40.0	32.0	40.0
23	36.69996905674649	39.0	35.0	40.0	32.0	40.0
24	36.597956026198624	38.0	35.0	40.0	31.0	40.0
25	36.53523920854034	38.0	35.0	40.0	31.0	40.0
26	36.34442763576352	38.0	35.0	40.0	31.0	40.0
27	36.27311546990769	38.0	35.0	40.0	31.0	40.0
28	36.162333465128675	38.0	35.0	40.0	31.0	40.0
29	36.02559264925822	38.0	35.0	40.0	31.0	40.0
30	35.87777844630486	38.0	35.0	40.0	30.0	40.0
31	35.713847105946265	38.0	34.0	40.0	30.0	40.0
32	35.58132058929707	38.0	34.0	40.0	30.0	40.0
33	35.26116020009971	37.0	34.0	40.0	28.0	40.0
34	35.10241615237833	37.0	34.0	40.0	27.0	40.0
35	35.017109040587236	37.0	33.0	40.0	28.0	40.0
36	34.83569218339035	36.0	33.0	40.0	27.0	40.0
37	34.74253665916006	36.0	33.0	40.0	27.0	40.0
38	34.30325333929277	36.0	33.0	40.0	26.0	40.0
39	34.31313197297622	35.0	33.0	39.0	26.0	40.0
40	34.106651939970085	35.0	33.0	39.0	25.0	40.0
41	33.77520070138041	35.0	33.0	39.0	23.0	40.0
42	33.76067198432209	35.0	33.0	39.0	24.0	40.0
43	33.75362122019563	35.0	33.0	39.0	24.0	40.0
44	33.60100135806501	35.0	33.0	39.0	24.0	40.0
45	33.46967733062866	35.0	32.0	38.0	23.0	40.0
46	33.19530608034931	35.0	32.0	38.0	23.0	40.0
47	32.85781918825531	35.0	31.0	38.0	23.0	40.0
48	32.78474239741452	35.0	31.0	38.0	23.0	40.0
49	32.71288786508742	35.0	31.0	37.0	23.0	40.0
50	32.57142648398687	35.0	31.0	37.0	23.0	40.0
51	32.98752041395197	35.0	32.0	37.0	24.0	40.0
52	32.8334754430902	35.0	32.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	6.0
15	23.0
16	98.0
17	215.0
18	495.0
19	921.0
20	1419.0
21	2399.0
22	3477.0
23	5178.0
24	7013.0
25	9653.0
26	12395.0
27	16340.0
28	19976.0
29	25420.0
30	31581.0
31	40730.0
32	52544.0
33	78271.0
34	130331.0
35	112187.0
36	137630.0
37	180813.0
38	241971.0
39	52328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.89085626858744	0.9226246755256056	0.13460315277371887	0.05191590311323512
8	98.63376940399856	1.0255109934503448	0.2193532860016159	0.12136631654948342
9	96.03891973663853	1.6491894586649705	1.2107407471076654	1.1011500575888329
10	56.59521067198432	29.542469615444123	6.48020491310103	7.382114799470527
11	35.26078286431383	24.88026679960805	20.891337608086506	18.96761272799161
12	31.425624452046552	17.53201767203589	26.840865723470458	24.201492152447095
13	25.608206838459026	16.66835708514552	28.337315844664868	29.38612023173059
14	26.322222413229962	19.30197177287652	29.230716336318785	25.14508947757474
15	26.980368224716784	19.382853999415516	28.211995667944507	25.424782107923193
16	30.91643602482337	20.502827869557	23.359663749978512	25.221072355641127
17	30.83916384452734	23.077134654724862	21.962146086537963	24.12155541420983
18	30.906981141806057	20.620154372453626	22.19542383661962	26.2774406491207
19	31.773736054047554	22.25498959962868	21.20059823623455	24.77067611008922
20	32.21347406783448	21.66655206202403	22.175052861391414	23.944921008750065
21	32.49712055835382	20.22511216929398	23.82862594763714	23.449141324715065
22	31.164927541214695	19.39574702171185	24.330250468446476	25.109074968626977
23	29.436059204758386	21.62546629763972	24.328531398806966	24.609943098794933
24	28.455587835863234	22.70383868250503	23.884753571367177	24.955819910264566
25	29.931752935311412	22.440133399804026	21.353853294596963	26.2742603702876
26	27.119870725963107	23.158962369565593	21.6149799728387	28.1061869316326
27	27.01199910608379	21.63973457564766	23.47148923002871	27.876777088239844
28	28.265114919805402	22.245792577057298	22.136889515394266	27.352202987743034
29	29.97060390916436	23.040002750511423	22.522390882054633	24.467002458269587
30	29.382080418077738	21.089546337522133	22.294270340891508	27.23410290350862
31	28.908734592838353	22.38495126437572	22.579034226676523	26.1272799161094
32	32.504168743875816	22.284041876536417	21.922951298757113	23.288838080830654
33	32.80724072132163	22.4205360059136	21.524986677210293	23.247236595554487
34	28.7879699506627	23.725223908820546	25.098502690343988	22.388303450172767
35	28.291588592253873	25.630726650736623	23.796221484932353	22.28146327207715
36	30.013064929260285	24.831874989255816	21.55541420982964	23.59964587165426
37	30.003352185797045	26.195355073834044	20.423664712657512	23.377628027711403
38	31.23076790840797	24.59533100685909	21.893984975331353	22.279916109401594
39	29.112788159048325	23.712846607416065	22.01655464062849	25.157810592907122
40	29.09989513675199	22.972873081088515	22.87333894896082	25.05389283319867
41	26.291537020164686	23.38235546922006	23.720668374275842	26.605439136339413
42	26.597617369479636	22.666191057399736	24.29191521548538	26.44427635763525
43	27.67633356827285	20.4370734558457	26.07639545477987	25.81019752110158
44	27.444087259974904	20.813033986006772	24.873218614086056	26.869660139932268
45	26.871465163053752	21.226126420381288	24.365921163466332	27.536487253098624
46	27.281635179041103	22.729366866651766	23.750408279039384	26.238589675267743
47	26.07639545477987	24.606247099069982	24.153702016468685	25.16365542968146
48	28.301129428753157	23.385707655017104	23.98291244778326	24.330250468446476
49	27.5207577658971	22.622612642038128	24.00551821354283	25.851111378521946
50	24.85001117395266	24.374516511663888	24.18395764212408	26.591514672259375
51	25.571074934245587	23.61821182376098	23.753244743944578	27.057468498048852
52	27.8934520637431	23.317460590328515	24.04402537346788	24.745061972460505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	13.0
10	26.0
11	24.5
12	23.0
13	144.5
14	822.5
15	1379.0
16	2020.5
17	2662.0
18	3543.0
19	4424.0
20	3541.0
21	2658.0
22	2806.5
23	2955.0
24	2857.0
25	2759.0
26	2724.0
27	2689.0
28	3011.5
29	3334.0
30	3680.0
31	4026.0
32	4700.0
33	5374.0
34	6433.5
35	7493.0
36	8298.5
37	9104.0
38	11137.0
39	15657.0
40	18144.0
41	18599.0
42	19054.0
43	20932.0
44	22810.0
45	25885.5
46	28961.0
47	29611.5
48	30262.0
49	32469.0
50	34676.0
51	37236.5
52	39797.0
53	41167.5
54	42538.0
55	47412.5
56	52287.0
57	58344.0
58	64401.0
59	67667.0
60	70933.0
61	82649.5
62	94366.0
63	105512.5
64	111880.0
65	107101.0
66	100704.5
67	94308.0
68	84843.0
69	75378.0
70	67988.5
71	60599.0
72	50298.5
73	39998.0
74	34384.5
75	28771.0
76	25117.0
77	21463.0
78	20833.5
79	20204.0
80	16113.0
81	12022.0
82	7822.5
83	3623.0
84	2678.5
85	1734.0
86	1213.5
87	693.0
88	427.0
89	109.0
90	57.0
91	44.0
92	31.0
93	25.0
94	19.0
95	17.0
96	15.0
97	14.0
98	13.0
99	6.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1163420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.757405204357172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83301978475284	9.54675156059769
2	9.19666034081686	2.346510449892844
3	3.5242228475630126	1.3487981669044447
4	1.736300259109294	0.8860274384755045
5	0.9111490331731361	0.5811948708873984
6	0.5490995078725134	0.4203050951665658
7	0.3826393751849921	0.3417039889463896
8	0.2657331852450867	0.27120527363328617
9	0.21181771788401474	0.24320200108577297
>10	3.953998252533654	14.308661430630188
>50	2.8584034392446336	26.04308647246338
>100	1.5081857117500865	33.08121382675498
>500	0.047662753870205896	4.130938789256162
>1k	0.018384205064222274	4.029741386415649
>5k	0.002723585935440337	2.420659248889761
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGGCAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9016	0.7749565934916024	No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7825	0.6725859964587165	No Hit
CGGCAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5526	0.474978941396916	No Hit
CGGCAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	5500	0.4727441508655516	No Hit
CGGCAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	4074	0.35017448556841035	No Hit
CGGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3298	0.2834745835553798	No Hit
CGGCAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2793	0.24006807515772463	No Hit
CGGCAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	2316	0.19906826425538496	No Hit
CGGCAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2165	0.1860892884770762	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1980	0.17018789431159856	No Hit
CGGCAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1930	0.16589022021282082	No Hit
CGGCAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1894	0.16279589486170085	No Hit
CGGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	1745	0.14998882604734318	No Hit
CGGCAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1668	0.14337040793522546	No Hit
CGGCAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1659	0.14259682659744546	No Hit
CGGCAGGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA	1649	0.1417372917776899	No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1600	0.13752557116088773	No Hit
CGGCAGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1479	0.1271251998418456	No Hit
CGGCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1468	0.12617971154011448	No Hit
CGGCAGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1437	0.12351515359887229	No Hit
CGGCAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1394	0.11981915387392343	No Hit
CGGCAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1362	0.1170686424507057	No Hit
CGGCAGGGGGGGGCTGACGAGATGGCTCAGTGGGTAAGAGCACTGACTGCTC	1286	0.11053617782056352	No Hit
CGGCAGGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1272	0.10933282907290573	No Hit
CGGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1255	0.10787161987932131	No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	1209	0.10391775970844579	No Hit
CGGCAGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1190	0.10228464355091026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.06936445995427275	0.0	0.0	0.0	0.0
6	0.06936445995427275	0.0	0.0	0.0	0.0
7	0.07159925048563717	0.0	0.0	0.0	0.0
8	0.08251534269653264	0.0	0.0	0.0	0.0
9	0.08750064465111482	0.0	0.0	0.0	0.0
10	0.1211944095855323	0.0	0.0	0.0	0.0
11	0.14130752436781213	0.0	0.0	0.0	0.0
12	0.15170789568685428	0.0	0.0	0.0	0.0
13	0.16219422048787197	0.0	0.0	0.0	0.0
14	0.18161970741434735	0.0	0.0	0.0	0.0
15	0.19726324113389834	0.0	0.0	0.0	0.0
16	0.21453989101098486	0.0	0.0	0.0	0.0
17	0.2342232383833869	0.0	0.0	0.0	0.0
18	0.24771793505354903	0.0	0.0	0.0	0.0
19	0.26877653813756	0.0	0.0	0.0	0.0
20	0.285365560158842	0.0	0.0	0.0	0.0
21	0.30049337298653966	0.0	0.0	0.0	0.0
22	0.31656667411596845	0.0	0.0	0.0	0.0
23	0.3371955097901016	0.0	0.0	0.0	0.0
24	0.3561052758247237	0.0	0.0	0.0	0.0
25	0.37174880954427464	0.0	0.0	0.0	0.0
26	0.38782211067370337	0.0	0.0	0.0	0.0
27	0.40123085386188995	0.0	0.0	0.0	0.0
28	0.42151587560812087	0.0	0.0	0.0	0.0
29	0.4332915026387719	0.0	0.0	0.0	0.0
30	0.4477316876106651	0.0	0.0	0.0	0.0
31	0.4599370820511939	0.0	0.0	0.0	0.0
32	0.4768699180003782	0.0	0.0	0.0	0.0
33	0.48933317288683364	0.0	0.0	0.0	0.0
34	0.5036874043767513	0.0	0.0	0.0	0.0
35	0.5255195887985422	0.0	0.0	0.0	0.0
36	0.5374671227931443	0.0	0.0	0.0	0.0
37	0.5519932612470131	0.0	0.0	0.0	0.0
38	0.5649722370253218	0.0	0.0	0.0	0.0
39	0.576919771019924	0.0	0.0	0.0	0.0
40	0.5878358632308195	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	6.3114864E-4	46.0	32
AGCGTAA	20	6.3114864E-4	46.0	25
CATATCG	20	6.3114864E-4	46.0	46
CGAAAGG	20	6.3114864E-4	46.0	30
TAGGTCG	25	3.4171826E-5	46.0	26
CCGCTTA	20	6.3114864E-4	46.0	11
TTATCGG	25	3.4171826E-5	46.0	17
TCGATAT	20	6.3114864E-4	46.0	42
GTAGACG	25	3.4171826E-5	46.0	10
TACGACA	20	6.3114864E-4	46.0	33
CACTAAT	25	3.4171826E-5	46.0	30
GTCGTAA	25	3.4171826E-5	46.0	15
TTAACGG	40	5.6115823E-9	46.0	13
CGATGAT	30	1.8614228E-6	46.0	30
CGCAAAC	25	3.4171826E-5	46.0	35
AATACGA	20	6.3114864E-4	46.0	34
ATATTCG	20	6.3114864E-4	46.0	43
TTGTACG	35	1.01957994E-7	45.999996	39
TACGTTC	35	1.01957994E-7	45.999996	35
TGCGTCG	235	0.0	45.02128	39
>>END_MODULE