FastQCFastQC Report
Fri 17 Jun 2016
SRR1527789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174961
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGA47272.7017449603054393No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17901.0230851446893878No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13420.767028080543664No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12240.6995844788267099No Hit
ACTTATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC9710.5549808242979865No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.39437360326015514No Hit
ACTTATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA5690.32521533370293954Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC5010.28634952932367785No Hit
ACTTATGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGT4940.2823486376964009No Hit
ACTTATGGGGAACTCGAGTGCGGCAACCTTTTCCCTAACTTGTGTGTGTT4890.2794908579626317No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGG4580.26177262361326237No Hit
ACTTATGGGAGTTCGCTGCTTTAAAGTGGCCATGCTTTCCCCTACGCTGT4260.24348283331713924No Hit
ACTTATGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3940.2251930430210161Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA3930.22462148707426227No Hit
ACTTATGGGAACAATCGCTTTCACCACTTCGTACATATCAACGTCACCGT3650.20861792056515455No Hit
ACTTATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA3620.20690325272489296No Hit
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.19375746594955445No Hit
ACTTATGGGTGCTACTTATGGGGGATGTCCAGCAATGAAAGGCCCCTTTG3380.19318591000280064No Hit
ACTTATGGGGAGACGGAGGAAGCTATGAACAATACAGGCAGTTTCAGCGT3350.19147124216253908No Hit
ACTTATGGGGGACTGGAGAAATGGCTCAGGGGTTAGGAACACTGACTGCT3330.1903281302690314No Hit
ACTTATGGGCGCAGAGTGCAGTGCTACTTATGGCCCGGAAGGGGATGAAC3300.18861346242876983No Hit
ACTTATGGGGACTATAAGCTTCCTCTCCTGGGGTTAAGGGGAGAACAGGG3270.1868987945885083No Hit
ACTTATGGGACCTCCAGACCCTCTGTGTGTCCCTGTCATCTGGGGATGGG3240.18518412674824675No Hit
ACTTATGGGGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCTTG3200.18289790296123135No Hit
ACTTATGGGACTTGGGCCCCGCCTTGGGGGCGCAGGACCCTAGGGCTCCT3190.18232634701447753No Hit
ACTTATGGGCCAACAGTGTCCTGACGGTCTCGACCTATACAGACCTCGCG3140.17946856728070826No Hit
ACTTATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA3030.17318145186641595Illumina Single End Adapter 2 (97% over 34bp)
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC2930.16746589239887746No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG2890.16517966861186206Illumina Single End Adapter 2 (100% over 23bp)
ACTTATGGGGGCGCGTGTGAGGCCGAGGTACCTCGAGCGTTTGTGCGCCG2850.1628934448248467No Hit
ACTTATGGGGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAAAA2770.1583209972508159No Hit
ACTTATGGGTATCGCCATTCAGTAATAACAGATTTTGTCATATTTCATAT2770.1583209972508159No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGT2750.1571778853573082No Hit
ACTTATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCT2700.15432010562353896No Hit
ACTTATGGGAGTGCAGTGCTACTTATGGGACTAAGTCCATTCCTTGAGCC2660.15203388183652355No Hit
ACTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT2580.14746143426249278No Hit
ACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCC2450.14003120695469276No Hit
ACTTATGGGGTTTTATCTGCATACATGTATGTGGACTACAAGCATGGAGT2450.14003120695469276No Hit
ACTTATGGGGCACAGGCGGAAGTCGCGGCACTCAAGAAGGCGTAAAAGCA2380.13603031532741583No Hit
ACTTATGGGGAGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAA2370.13545875938066199No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGC2300.13145786775338505No Hit
ACTTATGGGATCCGTGGCTTAAGTCAGGAGTGTAGCTTCATTTTCTTCTA2260.12917164396636965No Hit
ACTTATGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTC2260.12917164396636965No Hit
ACTTATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG2250.1286000880196158No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG2230.1274569761261081No Hit
ACTTATGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA2220.12688542017935425Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2180.12459919639233887Illumina Single End Adapter 2 (97% over 34bp)
ACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGT2160.12345608449883118No Hit
ACTTATGGGGGCCGAGGCAGATTTGGGGGACGCAGAACCTTGGTGGCGTT2130.12174141665856962No Hit
ACTTATGGGGGCCTTTTCCACACTGGCTGCCTTCGAGTGGTCCAGCCTGG2080.11888363692480039No Hit
ACTTATGGGGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCA2070.11831208097804653No Hit
ACTTATGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2070.11831208097804653Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGAGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAAA2060.11774052503129269No Hit
ACTTATGGGAGTGCAGTGCTACTTATGGGAACGCAGAGTGCAGTGCTACT2030.11602585719103113No Hit
ACTTATGGGTGGCTCGCGTGAGTGGTGGTGCGCCTGCGCCCTGTAAACGC2020.1154543012442773No Hit
ACTTATGGGAGATCAGGAGATCATTGAGGTGGATCCCGACACCAAGGAGA1990.11373963340401574No Hit
ACTTATTCGCCACCATGATTATGACCAGTGTTTCCAGTCCGTTCAGTTGT1980.1131680774572619No Hit
ACTTATGGGGAGAGTTAAAGTGGGAGGCCCCTGGCTTGGTCCCCTCCCGT1960.11202496556375421No Hit
ACTTATGGACAAGGAAGAAAGAAGAAAATGTGGATGAAGGAATCGAAAAT1920.10973874177673881No Hit
ACTTATGGGGCTATGGCTGTGCCTGATCCTAGCCAGAGAGGCCAGAAGAT1880.10745251798972343No Hit
ACTTATGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTC1880.10745251798972343No Hit
ACTTATGGGAGCTACTCTAGTATTACTAAATATTAGCCCACCAACAGCTA1870.10688096204296957No Hit
ACTTATGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.10630940609621573No Hit
ACTTATGGGGGTGGTATGTGTGTCTAATGAACAAAGGCTCTGTTGTCATT1830.10459473825595418No Hit
ACTTATGAGAGCACCATATGGTGGCTTAACAACTGTGCAGTTTCAGGGGA1820.10402318230920034No Hit
ACTTATGGGATGTCTTCCTGCCTGAAAAGCTCGTTAGCTGCGAGCACAGA1820.10402318230920034No Hit
ACTTATGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACA1810.10345162636244648No Hit
ACTTATGGGGACAGACAAGCCAACACGGCAGGCCAGGACAGAGCTAGAGA1770.10116540257543108No Hit
ACTTATGGGTGCTTTTGATTGAACCACCATAGACCTAGTAAGTGAGCCCA1750.10002229068192339No Hit
ACTTATGGGAAGCAGTGGTATCAACAGATCGGAAGAGCTCGTATGCCGTC1750.10002229068192339Illumina Single End Adapter 2 (100% over 25bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAA650.044.00000423
ATCTCTA650.044.00000425
CCCCTAC650.044.00000439
TCTCTAA650.044.00000426
CTTCATC650.044.00000421
TTTCGCC650.044.00000412
TGGGTCA650.044.0000046
TTATGAG1300.044.0000043
CGGCAAC650.044.00000421
CCTACGC650.044.00000441
TAACCAT650.044.00000430
CTACGCT650.044.00000442
CGCCGGA650.044.00000418
CTCTAAC650.044.00000427
AAGTGGC650.044.00000424
TCACGGC207.8381726E-444.021
CCTTCGC254.427333E-544.025
TGTCATA302.515384E-644.036
ATCCTCT254.427333E-544.018
CTTCGCT207.8381726E-444.010
Produced by FastQC (version 0.11.5)