FastQCFastQC Report
Fri 17 Jun 2016
SRR1527788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218702
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGAAG54362.485573977375607No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19670.89939735347642No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15070.6890654863695805No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13930.6369397627822334No Hit
ACTTATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG12250.5601229069693007No Hit
ACTTATGGGGAACTCGAGTGCGGCAACCTTTTCCCTAACTTGTGTGTGTTTT6490.2967508298963887No Hit
ACTTATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG6330.28943493886658556No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGGAG5800.2652010498303628No Hit
ACTTATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5470.25011202458139387Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT5390.2464540790664923No Hit
ACTTATGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGC5180.23685197208987574No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.22633537873453374No Hit
ACTTATGGGAGTTCGCTGCTTTAAAGTGGCCATGCTTTCCCCTACGCTGTGC4940.22587813554517105No Hit
ACTTATGGGAACAATCGCTTTCACCACTTCGTACATATCAACGTCACCGTTC4630.21170359667492755No Hit
ACTTATGGGACTTGGGCCCCGCCTTGGGGGCGCAGGACCCTAGGGCTCCTCC4550.20804565116002596No Hit
ACTTATGGGGACTATAAGCTTCCTCTCCTGGGGTTAAGGGGAGAACAGGGGT4460.2039304624557617No Hit
ACTTATGGGTGCTACTTATGGGGGATGTCCAGCAATGAAAGGCCCCTTTGGC4330.19798630099404668No Hit
ACTTATGGGGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCTTGTA4300.19661457142595862No Hit
ACTTATGGGGAGACGGAGGAAGCTATGAACAATACAGGCAGTTTCAGCGTCG4290.1961573282365959No Hit
ACTTATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA4180.19112765315360628No Hit
ACTTATGGGGGACTGGAGAAATGGCTCAGGGGTTAGGAACACTGACTGCTCT4050.18518349169189124No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTACTTATGGGAAG4040.18472624850252856No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC3890.17786760066208815No Hit
ACTTATGGGCCAACAGTGTCCTGACGGTCTCGACCTATACAGACCTCGCGAC3800.1737524119578239No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC3700.16917998006419696Illumina Single End Adapter 2 (100% over 25bp)
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3670.16780825049610887No Hit
ACTTATGGGCGCAGAGTGCAGTGCTACTTATGGCCCGGAAGGGGATGAACCG3630.1659792777386581No Hit
ACTTATGGGACCTCCAGACCCTCTGTGTGTCCCTGTCATCTGGGGATGGGGA3610.16506479135993268No Hit
ACTTATGGGAGTGCAGTGCTACTTATGGGACTAAGTCCATTCCTTGAGCCCC3560.16277857541311921No Hit
ACTTATGGGGGCGCGTGTGAGGCCGAGGTACCTCGAGCGTTTGTGCGCCGAG3540.16186408903439384No Hit
ACTTATGGGTATCGCCATTCAGTAATAACAGATTTTGTCATATTTCATATTC3400.1554626843833161No Hit
ACTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT3330.15226198205777725No Hit
ACTTATGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT3230.1476895501641503No Hit
ACTTATGGGGTTTTATCTGCATACATGTATGTGGACTACAAGCATGGAGTAC3200.14631782059606221No Hit
ACTTATGGGGCACAGGCGGAAGTCGCGGCACTCAAGAAGGCGTAAAAGCAGC3180.14540333421733684No Hit
ACTTATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC3150.14403160464924875No Hit
ACTTATGGGGGCCTTTTCCACACTGGCTGCCTTCGAGTGGTCCAGCCTGGCT3130.14311711827052334No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3050.1394591727556218No Hit
ACTTATGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2970.13580122724072025Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGTAG2930.1339722544832695No Hit
ACTTATGGGGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATG2870.1312287953470933No Hit
ACTTATGGGGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAA2810.12848533621091712No Hit
ACTTATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2800.12802809302155446Illumina Single End Adapter 2 (97% over 34bp)
ACTTATGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAG2780.12711360664282906No Hit
ACTTATGGGAGTGCAGTGCTACTTATGGGAACGCAGAGTGCAGTGCTACTTA2760.12619912026410365No Hit
ACTTATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC2760.12619912026410365No Hit
ACTTATGGGTGGCTCGCGTGAGTGGTGGTGCGCCTGCGCCCTGTAAACGCGG2750.12574187707474097No Hit
ACTTATGGGAAGCCCACATCCTCTGCACCAGGCACTCTTAAGTAGCTCTTTT2620.11979771561302593No Hit
ACTTATGGGGGCCGAGGCAGATTTGGGGGACGCAGAACCTTGGTGGCGTTGG2610.11934047242366326No Hit
ACTTATGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACG2600.11888322923430056No Hit
ACTTATGGGATATTTCTCCTGCTTGACGCTCCCTTACTAGTGTAGAGTCAGG2570.11751149966621247No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGCAG2570.11751149966621247No Hit
ACTTATGGGTCTACAGATGTATTGCAATCCCTGTCAAAATACTCAGGACATT2520.115225283719399No Hit
ACTTATGGGATGTCTTCCTGCCTGAAAAGCTCGTTAGCTGCGAGCACAGAAT2440.11156733820449743No Hit
ACTTATGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2430.11111009501513475Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGGCTATGGCTGTGCCTGATCCTAGCCAGAGAGGCCAGAAGATCT2390.10928112225768397No Hit
ACTTATGGACAAGGAAGAAAGAAGAAAATGTGGATGAAGGAATCGAAAATTC2380.10882387906832128No Hit
ACTTATGGGAGATCAGGAGATCATTGAGGTGGATCCCGACACCAAGGAGATG2380.10882387906832128No Hit
ACTTATGGGGAGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAAAA2350.1074521495002332No Hit
ACTTATGGGGGTGGTATGTGTGTCTAATGAACAAAGGCTCTGTTGTCATTTT2290.10470869036405704No Hit
ACTTATGGGAAGCCAGTCCCACCATGCAGAATGTGGAGCCTAGATTTTCATA2270.10379420398533164No Hit
ACTTATGGGAGCTACTCTAGTATTACTAAATATTAGCCCACCAACAGCTACC2270.10379420398533164No Hit
ACTTATGGGTCCACCACAACTCACCTGGAAGACGCTCTCAATGAAAATATTA2240.10242247441724354No Hit
ACTTATGGGGACCGCGCCACGCCGTCGCCCGCGCCGCTACCCCTCAATCTCC2200.10059350165979278No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGC750.046.00000415
CTCGAGT750.046.00000413
ATGAAAG750.046.00000435
TTCGCTG750.046.00000412
GTGCGGC750.046.00000418
CCCTGAC206.300196E-446.030
AACGTTC206.300196E-446.040
TGTCATA600.046.036
TTTAGGT253.4080243E-546.044
CTAGATA453.074092E-1046.019
CTTCGCT206.300196E-446.010
TTAGGTG253.4080243E-546.045
AAGTATA206.300196E-446.040
AACAATT301.8544488E-646.045
TAGAGTC301.8544488E-646.043
CTAGAGG301.8544488E-646.018
CGTGGGG206.300196E-446.038
AACAAGA253.4080243E-546.015
AGACTAT253.4080243E-546.018
TAATAGC253.4080243E-546.017
Produced by FastQC (version 0.11.5)