FastQCFastQC Report
Fri 17 Jun 2016
SRR1527787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219324
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGAAG51072.3285185387828053No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17910.8166000984844339No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13660.6228228556838286No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12920.5890828181138407No Hit
ACTTATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG11390.5193230107056228No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGGAG7770.3542703944848717No Hit
ACTTATGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG6700.305484123944484No Hit
ACTTATGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5920.26992030055990224Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCT5670.25852163921869015No Hit
ACTTATGGGGAACTCGAGTGCGGCAACCTTTTCCCTAACTTGTGTGTGTTTT5480.24985865659936896No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4870.22204592292681147No Hit
ACTTATGGGAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGC4770.21748645839032663No Hit
ACTTATGGGAGTTCGCTGCTTTAAAGTGGCCATGCTTTCCCCTACGCTGTGC4350.19833670733709033No Hit
ACTTATGGGGGACTGGAGAAATGGCTCAGGGGTTAGGAACACTGACTGCTCT4290.19560102861519943No Hit
ACTTATGGGGAGACGGAGGAAGCTATGAACAATACAGGCAGTTTCAGCGTCG4280.19514508216155096No Hit
ACTTATGGGAACAATCGCTTTCACCACTTCGTACATATCAACGTCACCGTTC4260.19423318925425398No Hit
ACTTATGGGTGCTACTTATGGGGGATGTCCAGCAATGAAAGGCCCCTTTGGC4260.19423318925425398No Hit
ACTTATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA4250.1937772428006055No Hit
ACTTATGGGGACTATAAGCTTCCTCTCCTGGGGTTAAGGGGAGAACAGGGGT4090.18648209954222977No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTACTTATGGGAAG4060.18511426018128432No Hit
ACTTATGGGGGGGTCGGCGGCGATGTCGGCTACCCACCCGACCCGTCTTGTA3990.18192263500574493No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC3950.180098849191151Illumina Single End Adapter 2 (100% over 25bp)
ACTTATGGGACTTGGGCCCCGCCTTGGGGGCGCAGGACCCTAGGGCTCCTCC3910.17827506337655707No Hit
ACTTATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.165964509128048No Hit
ACTTATGGGCCAACAGTGTCCTGACGGTCTCGACCTATACAGACCTCGCGAC3580.1632288304061571No Hit
ACTTATGGGTATCGCCATTCAGTAATAACAGATTTTGTCATATTTCATATTC3570.1627728839525086No Hit
ACTTATGGGGGCGCGTGTGAGGCCGAGGTACCTCGAGCGTTTGTGCGCCGAG3530.1609490981379147No Hit
ACTTATGGGCGCAGAGTGCAGTGCTACTTATGGCCCGGAAGGGGATGAACCG3480.15866936586967229No Hit
ACTTATGGGACCTCCAGACCCTCTGTGTGTCCCTGTCATCTGGGGATGGGGA3420.15593368714778136No Hit
ACTTATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT3370.15365395487953895No Hit
ACTTATGGGGCTTTTCAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCC3340.1522861155185935No Hit
ACTTATGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3280.1495504367967026Illumina Single End Adapter 2 (100% over 34bp)
ACTTATGGGAGTGCAGTGCTACTTATGGGACTAAGTCCATTCCTTGAGCCCC3280.1495504367967026No Hit
ACTTATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC3250.14818259743575715No Hit
ACTTATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3210.1463588116211632No Hit
ACTTATGGGGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAA3040.13860772190913898No Hit
ACTTATGGGGTTTTATCTGCATACATGTATGTGGACTACAAGCATGGAGTAC2950.13450420382630265No Hit
ACTTATGGGGCACAGGCGGAAGTCGCGGCACTCAAGAAGGCGTAAAAGCAGC2880.13131257865076326No Hit
ACTTATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC2880.13131257865076326No Hit
ACTTATGGGGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATG2850.1299447392898178No Hit
ACTTATGGGGGCCTTTTCCACACTGGCTGCCTTCGAGTGGTCCAGCCTGGCT2790.1272090605679269No Hit
ACTTATGGGAGTGCAGTGCTACTTATGGGAACGCAGAGTGCAGTGCTACTTA2760.12584122120698146No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGTAG2760.12584122120698146No Hit
ACTTATGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT2680.12219364957779359No Hit
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACTTATGGGCAG2540.11581039922671482No Hit
ACTTATGGGTGGCTCGCGTGAGTGGTGGTGCGCCTGCGCCCTGTAAACGCGG2450.11170688114387846No Hit
ACTTATGGGGGCCGAGGCAGATTTGGGGGACGCAGAACCTTGGTGGCGTTGG2440.11125093469023No Hit
ACTTATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2400.10942714887563604Illumina Single End Adapter 2 (97% over 34bp)
ACTTATGGGGAGTGCAGTGCTACTTATGGGAAAAAAAAAAAAAAAAAAAAAA2370.10805930951469059No Hit
ACTTATGGGCTTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACG2340.10669147015374514No Hit
ACTTATGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAG2310.10532363079279969No Hit
ACTTATGGGAGCTACTCTAGTATTACTAAATATTAGCCCACCAACAGCTACC2270.10349984497820576No Hit
ACTTATGGGGCTATGGCTGTGCCTGATCCTAGCCAGAGAGGCCAGAAGATCT2260.10304389852455727No Hit
ACTTATGGACAAGGAAGAAAGAAGAAAATGTGGATGAAGGAATCGAAAATTC2260.10304389852455727No Hit
ACTTATGGGATGTCTTCCTGCCTGAAAAGCTCGTTAGCTGCGAGCACAGAAT2250.1025879520709088No Hit
ACTTATGGGATATTTCTCCTGCTTGACGCTCCCTTACTAGTGTAGAGTCAGG2250.1025879520709088No Hit
ACTTATGGGAAGCCCACATCCTCTGCACCAGGCACTCTTAAGTAGCTCTTTT2240.10213200561726031No Hit
ACTTATGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG2240.10213200561726031No Hit
ACTTATGGGTCTACAGATGTATTGCAATCCCTGTCAAAATACTCAGGACATT2230.10167605916361182No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACCG206.300232E-446.00000437
TCCGGAC206.300232E-446.00000427
CCTTCGG351.01450496E-746.00000417
CTTAAGG206.300232E-446.00000442
TCGCCAA206.300232E-446.00000417
AACGTTC206.300232E-446.00000440
TAGAGTC206.300232E-446.00000443
TTCTAAG206.300232E-446.00000415
ATCTCTG351.01450496E-746.00000435
GACAGCC206.300232E-446.00000443
CGGCGCG206.300232E-446.00000431
CCTTAGT206.300232E-446.00000418
CTGTCCG206.300232E-446.00000441
TCGTTAG206.300232E-446.00000431
ACACGTC206.300232E-446.00000446
GCCCACG206.300232E-446.00000425
ACCGGCA206.300232E-446.00000432
TCCGCTT206.300232E-446.00000414
GATGTGC206.300232E-446.00000430
TCCGCCC206.300232E-446.00000444
Produced by FastQC (version 0.11.5)