FastQCFastQC Report
Fri 17 Jun 2016
SRR1527786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1356236
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG133770.9863327621446415No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC96200.7093160777327839No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT79000.582494492109043No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT62600.46157158488640615No Hit
CATGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC54610.4026585343553777No Hit
CATGATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG34570.25489664040771665No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT33290.24545875496594988No Hit
CATGATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC26500.1953937220365777No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG24030.17718155247316839No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG18140.13375253274503848No Hit
CATGATGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGA18120.13360506578501086No Hit
CATGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG16900.12460958122332691No Hit
CATGATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC15520.11443436098142212No Hit
CATGATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC15230.11229609006102183No Hit
CATGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC14350.10580754381980717No Hit
CATGATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG14170.10448034117955872No Hit
CATGATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA14020.10337433897935168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA207.856983E-444.015
ATCTACG254.4432665E-544.010
CGAAAAT207.856983E-444.016
TAACGCG408.314601E-944.034
TTAAGCG408.314601E-944.038
TAAGCGC408.314601E-944.039
TATCGGC254.4432665E-544.042
CGGACTA207.856983E-444.035
ATGGGAC74850.042.412835
CATGATG1401500.042.259151
ATGGGGT114350.042.2492375
ATGATGG1394500.042.2125472
TGATGGG1368850.042.1774443
GATGGGG815700.042.085084
ATGGGAT131650.042.0281075
GATGGGA417500.041.9976044
ATGGGGA222100.041.9198575
ATGGGGG337300.041.919365
ATGGGTA12500.041.8879975
ATGGGAG132350.041.440125
Produced by FastQC (version 0.11.5)