FastQCFastQC Report
Fri 17 Jun 2016
SRR1527785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049266
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT144790.7065456607390158No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC143670.7010802892352677No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG95940.46816762684785673No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC94490.46109192266889704No Hit
CATGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT81300.39672741362029135No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT56730.2768308262568159No Hit
CATGATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC51620.2518950687709648No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC47420.2313999256319092No Hit
CATGATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC39790.19416708226262477No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT37060.1808452392222386No Hit
CATGATGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT28950.14127009377991925No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT27940.13634149983457491No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT25490.12438599967012579No Hit
CATGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG24670.12038456696202444No Hit
CATGATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT24460.11935980980507167No Hit
CATGATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA23510.11472400361885671No Hit
CATGATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT21200.1034516748923761No Hit
CATGATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC21050.10271970549455267No Hit
CATGATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA21050.10271970549455267No Hit
CATGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG20940.10218292793614885No Hit
CATGATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG20700.10101177689963137No Hit
CATGATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA20580.10042620138137265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA206.312617E-446.046
TATATCG301.8621213E-646.024
CGATTAC206.312617E-446.020
GCCGATA206.312617E-446.010
CGAATAC206.312617E-446.037
CGATCGA301.8621213E-646.021
TATCGGC453.110472E-1046.042
CATGATG2120900.044.155361
ATGATGG2110550.044.0983622
ATGGGAT206250.044.0707865
TGATGGG2070900.044.041963
ATGGGAC109900.043.907195
GATGGGA636250.043.8201984
GATGGGG1230850.043.8043634
ATGGGGG502200.043.7604525
ATGGGGT172700.043.6960035
ATGGGGA336700.043.3495675
ATAGTCG850.043.29411314
ATGGGAG204700.043.146075
ATGGGGC227650.043.0397575
Produced by FastQC (version 0.11.5)