##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527785_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2049266
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28332973855029	33.0	31.0	34.0	31.0	34.0
2	32.27875785769149	33.0	31.0	34.0	31.0	34.0
3	32.790658704140895	34.0	31.0	34.0	31.0	34.0
4	36.32962338710543	37.0	37.0	37.0	35.0	37.0
5	35.802035460501465	37.0	35.0	37.0	35.0	37.0
6	36.2042306855235	37.0	37.0	37.0	35.0	37.0
7	36.526652957693145	37.0	37.0	37.0	35.0	37.0
8	36.6538536236877	37.0	37.0	37.0	35.0	37.0
9	38.70842731007102	39.0	39.0	39.0	39.0	39.0
10	38.144819169400165	39.0	38.0	39.0	37.0	39.0
11	37.81973399256124	39.0	38.0	39.0	35.0	39.0
12	37.25127338276241	39.0	37.0	39.0	35.0	39.0
13	37.11695895018021	39.0	37.0	39.0	34.0	39.0
14	37.90373968045144	40.0	37.0	40.0	34.0	40.0
15	37.929292732129454	40.0	37.0	40.0	34.0	40.0
16	37.96107045156656	40.0	37.0	40.0	34.0	40.0
17	37.88645056327485	40.0	37.0	40.0	33.0	40.0
18	37.858123835558686	40.0	37.0	40.0	33.0	40.0
19	37.84266610581545	40.0	37.0	40.0	34.0	40.0
20	37.85074802392661	40.0	37.0	40.0	34.0	40.0
21	37.813029640856776	40.0	37.0	40.0	33.0	40.0
22	37.80708214550966	40.0	37.0	40.0	34.0	40.0
23	37.787607855690766	40.0	37.0	40.0	34.0	40.0
24	37.70673060500687	40.0	37.0	40.0	34.0	40.0
25	37.71555474008743	40.0	37.0	40.0	34.0	40.0
26	37.58559991723866	40.0	36.0	40.0	33.0	40.0
27	37.50503790137542	40.0	36.0	40.0	33.0	40.0
28	37.394621293672955	40.0	36.0	40.0	33.0	40.0
29	37.35057967096512	40.0	36.0	40.0	33.0	40.0
30	37.2105197665896	40.0	35.0	40.0	33.0	40.0
31	37.107645859541904	39.0	35.0	40.0	32.0	40.0
32	37.00764273647248	39.0	35.0	40.0	32.0	40.0
33	36.891296200688444	39.0	35.0	40.0	32.0	40.0
34	36.74873979268675	39.0	35.0	40.0	31.0	40.0
35	36.5400870360412	39.0	35.0	40.0	31.0	40.0
36	36.577936197643446	39.0	35.0	40.0	31.0	40.0
37	36.53500472852231	39.0	35.0	40.0	31.0	40.0
38	36.474903697226225	39.0	35.0	40.0	31.0	40.0
39	36.37062343297551	38.0	35.0	40.0	31.0	40.0
40	36.13271971525415	38.0	35.0	40.0	30.0	40.0
41	36.030158603129124	38.0	35.0	40.0	30.0	40.0
42	35.928693981162034	38.0	35.0	40.0	30.0	40.0
43	35.829945941620075	38.0	35.0	40.0	30.0	40.0
44	35.656684393338885	38.0	34.0	40.0	30.0	40.0
45	35.557077509703475	38.0	34.0	40.0	29.0	40.0
46	35.37939730615742	37.0	34.0	40.0	29.0	40.0
47	35.21680201594132	37.0	34.0	40.0	28.0	40.0
48	35.03346466490929	37.0	34.0	40.0	28.0	40.0
49	35.336226727032994	37.0	34.0	40.0	29.0	40.0
50	35.392359020254084	37.0	34.0	40.0	29.0	40.0
51	35.42740376310347	37.0	34.0	40.0	30.0	40.0
52	34.941777202178734	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	7.0
16	39.0
17	111.0
18	240.0
19	491.0
20	907.0
21	1526.0
22	2558.0
23	3961.0
24	5995.0
25	8282.0
26	11571.0
27	15823.0
28	20560.0
29	27049.0
30	34678.0
31	43915.0
32	56360.0
33	79521.0
34	158611.0
35	163389.0
36	185909.0
37	300321.0
38	622128.0
39	305307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.32126917637827	0.5379487094403558	0.060851055939053295	0.079931058242317
8	99.342545086875	0.5309218032212509	0.08583561138475923	0.04069749851898192
9	97.95370635144486	1.1772507814993272	0.42190716090541686	0.44713570615039727
10	58.565896277008456	30.513461893185166	4.565829911783048	6.354811918023332
11	36.85597672532507	24.67551796594488	20.916367128523092	17.55213818020696
12	32.789984316335705	16.623610600088032	26.49817056448504	24.088234519091227
13	26.774562209103163	17.273501829435514	28.52748252301068	27.424453438450648
14	25.772496103482904	18.268248241077536	29.977611496018575	25.98164415942098
15	26.26833217356849	17.86137085180743	29.280776629290685	26.5895203453334
16	31.172283149186097	19.03798725982864	26.342749062347202	23.446980528638058
17	30.69401434464828	19.42632142435389	23.935057723106713	25.944606507891116
18	29.503929699707115	19.46760449839113	24.832013023199526	26.196452778702227
19	29.293561694772663	21.172263630002156	24.249365382532087	25.28480929269309
20	30.69162324461539	21.010156807364197	23.600352516461992	24.69786743155842
21	30.255369483512634	21.482130675080736	24.2863542361021	23.976145605304534
22	29.90397537459754	20.42702118709821	24.418059929750456	25.250943508553796
23	29.972438912273958	21.500234718186903	24.653754075849594	23.873572293689545
24	29.99322684317214	20.161950669166423	24.06603144735725	25.77879104030419
25	29.16756536242733	21.01337747271462	22.83110147730944	26.987955687548613
26	28.483320369341996	20.875425640204835	23.886942934689788	26.75431105576338
27	26.899728976130966	19.08673642172368	24.078767714879376	29.93476688726598
28	28.211613328869944	22.42964066158322	22.605996488498807	26.752749521048024
29	30.209645795128598	21.201054426316546	22.101376785639346	26.487922992915514
30	28.668313435151905	20.902850093643284	23.55877665466562	26.87005981653919
31	30.70889772240402	21.591535701075408	22.479658570434488	25.219908006086083
32	32.55902357234249	20.78197754708271	22.968760522060094	23.690238358514705
33	33.33364238707908	20.236611547744413	22.587843647432788	23.841902417743718
34	30.244877922143832	21.94619927330078	24.95508147795357	22.853841326601817
35	28.23918417618796	23.959993480592566	26.09217153849232	21.708650804727156
36	28.31643134663826	24.732465185095542	23.340356986355115	23.610746481911086
37	29.219827977431915	25.4474040949296	21.875588625390748	23.45717930224773
38	31.055363237373772	25.31389287676661	21.418644529309518	22.2120993565501
39	30.731100794138	23.2175813193602	21.687179702391003	24.364138184110796
40	28.01432317717661	23.70185227296017	22.064241538189773	26.219583011673446
41	26.85390769182722	23.359924968256927	22.899857802744982	26.88630953717087
42	25.916938064653394	23.003163083757794	22.9723227731295	28.107576078459314
43	25.952023797789064	21.403614757674212	24.548252886643315	28.096108557893412
44	25.930796685252183	21.335297613877362	24.16148025683342	28.572425444037037
45	27.27288697514134	21.175825881071564	23.750113455256663	27.80117368853043
46	27.869832418046265	22.926989468424306	23.197574155819694	26.005603957709738
47	27.394345097220175	22.7255514901433	23.55726391791012	26.322839494726402
48	27.457782445031537	22.122018322657965	24.816690463805088	25.603508768505407
49	28.07146558816669	21.454071848164173	24.352865855384316	26.121596708284823
50	26.269991304203554	22.020908949838624	25.680609545076138	26.02849020088168
51	25.628883707629953	22.210245034075616	25.045992077163238	27.114879181131197
52	25.956562008055567	22.426615188072216	25.06028987940072	26.556532924471494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.0
4	12.0
5	10.0
6	8.0
7	29.0
8	50.0
9	337.0
10	624.0
11	1757.5
12	2891.0
13	3509.5
14	3700.0
15	3272.0
16	3400.0
17	3528.0
18	3836.0
19	4144.0
20	3962.0
21	3780.0
22	4298.5
23	4817.0
24	6246.0
25	7675.0
26	8431.5
27	9188.0
28	11664.5
29	14141.0
30	16613.5
31	19086.0
32	21079.5
33	23073.0
34	24552.0
35	26031.0
36	30691.5
37	35352.0
38	37773.0
39	43578.0
40	46962.0
41	52689.5
42	58417.0
43	63400.5
44	68384.0
45	70703.5
46	73023.0
47	78388.5
48	83754.0
49	88224.0
50	92694.0
51	102022.5
52	111351.0
53	119665.5
54	127980.0
55	139755.5
56	151531.0
57	167915.0
58	184299.0
59	187206.0
60	190113.0
61	184918.5
62	179724.0
63	158425.0
64	126820.0
65	116514.0
66	92721.0
67	68928.0
68	60710.0
69	52492.0
70	45834.5
71	39177.0
72	37222.0
73	35267.0
74	26380.0
75	17493.0
76	11745.0
77	5997.0
78	4782.5
79	3568.0
80	2536.5
81	1505.0
82	1079.5
83	654.0
84	434.5
85	215.0
86	135.5
87	56.0
88	46.0
89	23.5
90	11.0
91	6.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2049266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.903549629439449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.09561023823512	5.302934854354168
2	9.426217560139431	1.4900115660891124
3	4.29543528151338	1.0184755778245864
4	2.2333642786693004	0.706060216683202
5	1.2650521384052402	0.49992011798571595
6	0.7918060679679912	0.3754847133045795
7	0.5265705529859764	0.2913243549248227
8	0.3985642066684951	0.25200575903380895
9	0.321997984105646	0.2290434343162585
>10	6.090684922946738	13.786687605606149
>50	4.777725101914496	27.01767661043375
>100	2.6540858844550206	36.49164822934598
>500	0.08274720998533569	4.3972003697661
>1k	0.035815956560816944	4.8786688217255945
>5k	0.003087582462139391	1.855007898842012
>10k+	0.0012350329848557567	1.4078498697641728
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	14479	0.7065456607390158	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14367	0.7010802892352677	No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9594	0.46816762684785673	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9449	0.46109192266889704	No Hit
CATGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	8130	0.39672741362029135	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5673	0.2768308262568159	No Hit
CATGATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5162	0.2518950687709648	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4742	0.2313999256319092	No Hit
CATGATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3979	0.19416708226262477	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3706	0.1808452392222386	No Hit
CATGATGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	2895	0.14127009377991925	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	2794	0.13634149983457491	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2549	0.12438599967012579	No Hit
CATGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2467	0.12038456696202444	No Hit
CATGATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	2446	0.11935980980507167	No Hit
CATGATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2351	0.11472400361885671	No Hit
CATGATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2120	0.1034516748923761	No Hit
CATGATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2105	0.10271970549455267	No Hit
CATGATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2105	0.10271970549455267	No Hit
CATGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2094	0.10218292793614885	No Hit
CATGATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2070	0.10101177689963137	No Hit
CATGATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	2058	0.10042620138137265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.03694005561015505	0.0	0.0	0.0	0.0
8	0.05426333135864256	0.0	0.0	0.0	0.0
9	0.0617294192164414	0.0	0.0	0.0	0.0
10	0.09091059920966824	0.0	0.0	0.0	0.0
11	0.10711152188149319	0.0	0.0	0.0	0.0
12	0.1164807301736329	0.0	0.0	0.0	0.0
13	0.12633791806432157	0.0	0.0	0.0	0.0
14	0.13712226719225323	0.0	0.0	0.0	0.0
15	0.1443443652507776	0.0	0.0	0.0	0.0
16	0.15537270417798374	0.0	0.0	0.0	0.0
17	0.16620585126577028	0.0	0.0	0.0	0.0
18	0.1756726554776198	0.0	0.0	0.0	0.0
19	0.18840892299974724	0.0	0.0	0.0	0.0
20	0.19870529252912994	0.0	0.0	0.0	0.0
21	0.20919685389793224	0.0	0.0	0.0	0.0
22	0.21944442546746007	0.0	0.0	0.0	0.0
23	0.23242468278886197	0.0	0.0	0.0	0.0
24	0.24525854623069918	0.0	0.0	0.0	0.0
25	0.25692125863601895	0.0	0.0	0.0	0.0
26	0.27019430371655023	0.0	0.0	0.0	0.0
27	0.2815642283627406	0.0	0.0	0.0	0.0
28	0.2988387061513732	0.0	0.0	0.0	0.0
29	0.31059901447640276	0.0	0.0	0.0	0.0
30	0.32304249423940085	0.0	0.0	0.0	0.0
31	0.33436362092573635	0.0	0.0	0.0	0.0
32	0.34627032313033057	0.0	0.0	0.0	0.0
33	0.3556883293823252	0.0	0.0	0.0	0.0
34	0.3661798907511275	0.0	0.0	0.0	0.0
35	0.3842351358974384	0.0	0.0	0.0	0.0
36	0.39965529121158505	0.0	0.0	0.0	0.0
37	0.414441073047618	0.0	0.0	0.0	0.0
38	0.4257134017740986	0.0	0.0	0.0	0.0
39	0.43742491213927326	0.0	0.0	0.0	0.0
40	0.4502099776212556	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATTA	20	6.312617E-4	46.0	46
TATATCG	30	1.8621213E-6	46.0	24
CGATTAC	20	6.312617E-4	46.0	20
GCCGATA	20	6.312617E-4	46.0	10
CGAATAC	20	6.312617E-4	46.0	37
CGATCGA	30	1.8621213E-6	46.0	21
TATCGGC	45	3.110472E-10	46.0	42
CATGATG	212090	0.0	44.15536	1
ATGATGG	211055	0.0	44.098362	2
ATGGGAT	20625	0.0	44.070786	5
TGATGGG	207090	0.0	44.04196	3
ATGGGAC	10990	0.0	43.90719	5
GATGGGA	63625	0.0	43.820198	4
GATGGGG	123085	0.0	43.804363	4
ATGGGGG	50220	0.0	43.760452	5
ATGGGGT	17270	0.0	43.696003	5
ATGGGGA	33670	0.0	43.349567	5
ATAGTCG	85	0.0	43.294113	14
ATGGGAG	20470	0.0	43.14607	5
ATGGGGC	22765	0.0	43.039757	5
>>END_MODULE