FastQCFastQC Report
Fri 17 Jun 2016
SRR1527784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2063116
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT141830.6874552860818296No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC137330.6656436186816447No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC91790.44490954459177284No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG90280.43759051841971075No Hit
CATGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT78480.38039547945922575No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT55330.2681865682782742No Hit
CATGATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC49480.23983140065803377No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC44220.21433598498581757No Hit
CATGATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC38320.18573846550557507No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT36100.1749780429214838No Hit
CATGATGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT27700.13426293044113854No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT26330.12762248947708224No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT24790.12015805218901893No Hit
CATGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23940.116038070568984No Hit
CATGATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT23590.11434160754896963No Hit
CATGATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA21780.10556847021689522No Hit
CATGATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA20700.10033367004085084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGT351.02010745E-746.00000430
ATACGCA351.02010745E-746.00000410
TAATACG206.312627E-446.018
ATAGCGT206.312627E-446.031
TACGAAC253.4181074E-546.016
TATCGGC301.8621286E-646.042
CATGATG2129800.044.2354161
ATGATGG2119100.044.1885222
TGATGGG2080250.044.132583
ATGGGAT206550.044.0401845
GATGGGA638050.043.9272774
GATGGGG1238650.043.918464
ATGGGGG501100.043.8519255
ATGGGGT171700.043.6557925
ATGGGGA338850.043.610745
ATGGGAC113750.043.472535
ATGGGAG203000.043.3714265
ATGGGGC235000.043.200855
CACGTCA750.042.9333346
TGGGGGG242550.042.6336866