##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2063116
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.227726894658375	33.0	31.0	34.0	31.0	34.0
2	32.24621155572445	33.0	31.0	34.0	31.0	34.0
3	32.768202563501035	34.0	31.0	34.0	31.0	34.0
4	36.31645918116092	37.0	37.0	37.0	35.0	37.0
5	35.81653770316356	37.0	35.0	37.0	35.0	37.0
6	36.15026736257195	37.0	36.0	37.0	35.0	37.0
7	36.48349341481526	37.0	37.0	37.0	35.0	37.0
8	36.626996736974554	37.0	37.0	37.0	35.0	37.0
9	38.664649976055635	39.0	39.0	39.0	38.0	39.0
10	38.05700503510224	39.0	38.0	39.0	37.0	39.0
11	37.78623645010751	39.0	38.0	39.0	35.0	39.0
12	37.198137671367	39.0	37.0	39.0	34.0	39.0
13	36.92297088481695	39.0	37.0	39.0	33.0	39.0
14	37.84040499904029	40.0	37.0	40.0	33.0	40.0
15	37.87871743518057	40.0	37.0	40.0	33.0	40.0
16	37.93738403463499	40.0	37.0	40.0	34.0	40.0
17	37.89763445196489	40.0	37.0	40.0	34.0	40.0
18	37.875783038859666	40.0	37.0	40.0	34.0	40.0
19	37.820969833979284	40.0	37.0	40.0	34.0	40.0
20	37.83566023432517	40.0	37.0	40.0	34.0	40.0
21	37.86096564613914	40.0	37.0	40.0	34.0	40.0
22	37.74559161966656	40.0	37.0	40.0	33.0	40.0
23	37.752174380887936	40.0	37.0	40.0	34.0	40.0
24	37.71283631167613	40.0	37.0	40.0	34.0	40.0
25	37.69384271170404	40.0	36.0	40.0	34.0	40.0
26	37.51199108532918	40.0	36.0	40.0	33.0	40.0
27	37.436087452183976	40.0	36.0	40.0	33.0	40.0
28	37.35235827747931	40.0	36.0	40.0	33.0	40.0
29	37.30758134782533	40.0	35.0	40.0	33.0	40.0
30	37.15306361833266	40.0	35.0	40.0	32.0	40.0
31	37.07053602414988	39.0	35.0	40.0	32.0	40.0
32	36.970864459390555	39.0	35.0	40.0	32.0	40.0
33	36.70403942386177	39.0	35.0	40.0	31.0	40.0
34	36.65291384488318	39.0	35.0	40.0	31.0	40.0
35	36.52593067961278	39.0	35.0	40.0	31.0	40.0
36	36.41647827848749	39.0	35.0	40.0	31.0	40.0
37	36.45487796129738	39.0	35.0	40.0	31.0	40.0
38	36.11959724998498	38.0	35.0	40.0	30.0	40.0
39	36.18976732282625	38.0	35.0	40.0	30.0	40.0
40	36.037979444684645	38.0	35.0	40.0	30.0	40.0
41	35.72701922722717	38.0	35.0	40.0	30.0	40.0
42	35.772146112966986	38.0	35.0	40.0	30.0	40.0
43	35.78371647546721	38.0	35.0	40.0	30.0	40.0
44	35.611213814443786	38.0	34.0	40.0	29.0	40.0
45	35.50485430775584	38.0	34.0	40.0	29.0	40.0
46	35.15965025718379	37.0	34.0	40.0	28.0	40.0
47	34.91480168832	37.0	34.0	40.0	27.0	40.0
48	34.90127312279096	37.0	33.0	40.0	27.0	40.0
49	34.82031887688331	36.0	33.0	40.0	27.0	40.0
50	34.65231378167781	36.0	33.0	40.0	27.0	40.0
51	35.0327834208062	36.0	34.0	40.0	28.0	40.0
52	34.77120191012042	36.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	3.0
15	5.0
16	47.0
17	136.0
18	265.0
19	540.0
20	1022.0
21	1697.0
22	2786.0
23	4382.0
24	6221.0
25	8735.0
26	12158.0
27	16296.0
28	21676.0
29	28523.0
30	36015.0
31	45899.0
32	59510.0
33	86080.0
34	163385.0
35	166270.0
36	198830.0
37	318709.0
38	619233.0
39	264687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.3033838136101	0.5525137704326853	0.06645288001256353	0.07764953594465847
8	99.33455026280636	0.533852677212527	0.08836148815674931	0.04323557182436664
9	97.91073308529428	1.19692736617815	0.4347792368436869	0.4575603116838801
10	58.55933452118058	30.457909298362285	4.601534765859021	6.381221414598112
11	36.839033772216396	24.65343684019706	20.94235127835759	17.565178109228953
12	32.804117655042184	16.564264927420467	26.56632007119328	24.065297346344074
13	26.981905040724808	17.234270879582148	28.441735704633185	27.34208837505986
14	25.80548064190283	18.297759311643166	29.945286644085932	25.95147340236807
15	26.262410838750704	17.854352348583404	29.353221050100913	26.53001576256497
16	31.177258089220384	19.04992254434554	26.3679793089676	23.404840057466473
17	30.770349316276935	19.38228388515236	23.937141682774985	25.910225115795722
18	29.48462422859403	19.464586576809058	24.82763935716654	26.22314983743037
19	29.317013682216608	21.16962885266752	24.23823963364154	25.27511783147433
20	30.674087157484113	21.03667462227039	23.573613892771906	24.71562432747359
21	30.274448940340726	21.41857268326163	24.30212358393808	24.00485479245956
22	30.05531438852687	20.483724618489703	24.36688000093063	25.0940809920528
23	29.963996207678097	21.50489841579436	24.70403990856549	23.827065467962054
24	30.031224613642664	20.08699462366634	24.125739900228584	25.75604086246241
25	29.227149612527846	21.022666684762275	22.768617954589075	26.981565748120808
26	28.58147578711037	20.82907601899263	23.871318917598426	26.71812927629857
27	26.97056297367671	19.06761423012569	24.078723639388187	29.883099156809408
28	28.19778432235512	22.35109417017754	22.659317265728152	26.791804241739193
29	30.263397695524635	21.222413087775966	22.046021648806953	26.468167567892447
30	28.729213480967626	20.901296873273242	23.546809777055678	26.82267986870346
31	30.76579310130889	21.583032655459025	22.44784103269036	25.20333321054172
32	32.4883816518315	20.86000011632889	22.997010347455017	23.65460788438459
33	33.34179949164274	20.264832418535846	22.580068207507477	23.813299882313938
34	30.26412475110464	21.946075741742103	24.932093008827426	22.857706498325832
35	28.302431855504008	23.90941662999075	26.097272281345308	21.690879233159936
36	28.50130579182169	24.765112577286008	23.2781385050574	23.4554431258349
37	29.282018073632308	25.438899218463725	21.860428594417378	23.41865411348659
38	31.059184263027383	25.336772144658852	21.372283478001236	22.231760114312525
39	30.782321498161036	23.211637154672836	21.608382660015238	24.39765868715089
40	28.121831249430475	23.630178816896382	22.03671533738287	26.211274596290274
41	27.04525581692934	23.34764501850599	22.8655102282179	26.741588936346766
42	25.918416608663787	22.99773740303502	22.91034532231828	28.17350066598291
43	25.864275203139332	21.468739518282053	24.560034433352268	28.106950845226347
44	25.957532198868122	21.32895096543287	24.17236839809298	28.54114843760603
45	27.224159959982863	21.197644727683755	23.758867654557474	27.819327657775904
46	28.040740317073787	22.884995317762062	23.165638771644446	25.90862559351971
47	27.56980218271779	22.748260398348904	23.51278357591139	26.169153843021913
48	27.45211611950079	22.132783614687686	24.84455551699468	25.570544748816836
49	28.07665686272609	21.507903578858386	24.33013945895432	26.085300099461207
50	26.37515292402366	22.008844873482637	25.625170858061303	25.9908313444324
51	25.672671822621705	22.199381905816253	25.056855746356483	27.071090525205566
52	25.932133723939906	22.333111662165383	25.08477468062872	26.649979933265993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	10.0
5	9.5
6	9.0
7	35.5
8	62.0
9	320.5
10	579.0
11	1697.5
12	2816.0
13	3454.5
14	3687.0
15	3281.0
16	3410.5
17	3540.0
18	3839.0
19	4138.0
20	3916.5
21	3695.0
22	4304.0
23	4913.0
24	6301.5
25	7690.0
26	8423.5
27	9157.0
28	11515.0
29	13873.0
30	16512.0
31	19151.0
32	21118.0
33	23085.0
34	24783.0
35	26481.0
36	30944.5
37	35408.0
38	38182.5
39	43905.0
40	46853.0
41	52731.5
42	58610.0
43	63480.5
44	68351.0
45	70881.5
46	73412.0
47	78985.5
48	84559.0
49	88834.0
50	93109.0
51	102834.5
52	112560.0
53	120653.0
54	128746.0
55	141540.0
56	154334.0
57	169764.0
58	185194.0
59	188486.0
60	191778.0
61	186389.0
62	181000.0
63	159862.0
64	127513.5
65	116303.0
66	93033.5
67	69764.0
68	61088.0
69	52412.0
70	46024.5
71	39637.0
72	37354.0
73	35071.0
74	26250.0
75	17429.0
76	11839.0
77	6249.0
78	4874.0
79	3499.0
80	2541.0
81	1583.0
82	1124.0
83	665.0
84	435.0
85	205.0
86	138.5
87	72.0
88	55.5
89	29.5
90	20.0
91	10.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2063116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.274103177379672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.75257333354749	5.523176791173748
2	9.931034553761819	1.6434080911189597
3	4.226048856431757	1.0490029281229118
4	2.2636713885457938	0.7491940251404081
5	1.2792842865899898	0.5292465090223061
6	0.7668121400622533	0.3806809658725438
7	0.5393998363568393	0.312413492991474
8	0.41987913420548206	0.2779298622756004
9	0.30419589801961944	0.22652534217149964
>10	6.328602819729409	15.026645545734821
>50	4.636393802097922	27.259523622909892
>100	2.4342295319542187	34.7030826417646
>500	0.07981080432636783	4.333554873972189
>1k	0.03438003878674306	4.9629822449254
>5k	0.002455717056195933	1.6045800818069593
>10k+	0.0012278585280979665	1.4180529809966782
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	14183	0.6874552860818296	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13733	0.6656436186816447	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9179	0.44490954459177284	No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	9028	0.43759051841971075	No Hit
CATGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7848	0.38039547945922575	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5533	0.2681865682782742	No Hit
CATGATGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	4948	0.23983140065803377	No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	4422	0.21433598498581757	No Hit
CATGATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3832	0.18573846550557507	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3610	0.1749780429214838	No Hit
CATGATGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT	2770	0.13426293044113854	No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT	2633	0.12762248947708224	No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2479	0.12015805218901893	No Hit
CATGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2394	0.116038070568984	No Hit
CATGATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	2359	0.11434160754896963	No Hit
CATGATGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2178	0.10556847021689522	No Hit
CATGATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2070	0.10033367004085084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.037709949416319784	0.0	0.0	0.0	0.0
8	0.05481999073246487	0.0	0.0	0.0	0.0
9	0.06281760211253269	0.0	0.0	0.0	0.0
10	0.08991254006076246	0.0	0.0	0.0	0.0
11	0.10571388133289646	0.0	0.0	0.0	0.0
12	0.11521407424497701	0.0	0.0	0.0	0.0
13	0.12340556711304648	0.0	0.0	0.0	0.0
14	0.13348740448913196	0.0	0.0	0.0	0.0
15	0.14250289368120841	0.0	0.0	0.0	0.0
16	0.15355413849730215	0.0	0.0	0.0	0.0
17	0.16378138698938888	0.0	0.0	0.0	0.0
18	0.17376628362147353	0.0	0.0	0.0	0.0
19	0.18738645815358904	0.0	0.0	0.0	0.0
20	0.19843770296968274	0.0	0.0	0.0	0.0
21	0.20856801071776865	0.0	0.0	0.0	0.0
22	0.21918302218585867	0.0	0.0	0.0	0.0
23	0.23130061518596143	0.0	0.0	0.0	0.0
24	0.2439513822780687	0.0	0.0	0.0	0.0
25	0.2540816900261546	0.0	0.0	0.0	0.0
26	0.26862280162627794	0.0	0.0	0.0	0.0
27	0.27923781309436796	0.0	0.0	0.0	0.0
28	0.2971233803625196	0.0	0.0	0.0	0.0
29	0.3071082769946043	0.0	0.0	0.0	0.0
30	0.3200013959467136	0.0	0.0	0.0	0.0
31	0.3295500592307946	0.0	0.0	0.0	0.0
32	0.3422492966949023	0.0	0.0	0.0	0.0
33	0.3525734859309898	0.0	0.0	0.0	0.0
34	0.3651273122790963	0.0	0.0	0.0	0.0
35	0.38344911289525163	0.0	0.0	0.0	0.0
36	0.3982810467273774	0.0	0.0	0.0	0.0
37	0.4118042805154921	0.0	0.0	0.0	0.0
38	0.4223708216115817	0.0	0.0	0.0	0.0
39	0.4341491220076816	0.0	0.0	0.0	0.0
40	0.44592742240378147	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGT	35	1.02010745E-7	46.000004	30
ATACGCA	35	1.02010745E-7	46.000004	10
TAATACG	20	6.312627E-4	46.0	18
ATAGCGT	20	6.312627E-4	46.0	31
TACGAAC	25	3.4181074E-5	46.0	16
TATCGGC	30	1.8621286E-6	46.0	42
CATGATG	212980	0.0	44.235416	1
ATGATGG	211910	0.0	44.188522	2
TGATGGG	208025	0.0	44.13258	3
ATGGGAT	20655	0.0	44.040184	5
GATGGGA	63805	0.0	43.927277	4
GATGGGG	123865	0.0	43.91846	4
ATGGGGG	50110	0.0	43.851925	5
ATGGGGT	17170	0.0	43.655792	5
ATGGGGA	33885	0.0	43.61074	5
ATGGGAC	11375	0.0	43.47253	5
ATGGGAG	20300	0.0	43.371426	5
ATGGGGC	23500	0.0	43.20085	5
CACGTCA	75	0.0	42.93333	46
TGGGGGG	24255	0.0	42.633686	6
>>END_MODULE