FastQCFastQC Report
Fri 17 Jun 2016
SRR1527783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325061
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC17040.5242093022540385No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC13380.41161505071355836No Hit
GGCTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC9170.28210089798530125No Hit
GGCTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8830.2716413227055845No Hit
GGCTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC8290.25502905608485793No Hit
GGCTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC7050.2168823697705969No Hit
GGCTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG6420.19750139204641592No Hit
GGCTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA6340.19504031551001197No Hit
GGCTGCGGGACGTACTTATTTCTCTGCTTGACGGGATGGGATGTTACCTG6130.18857998960195163No Hit
GGCTGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA6090.18734945133374967No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG5650.173813530383528No Hit
GGCTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC5510.16950664644482114No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT5450.1676608390425182No Hit
GGCTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4940.15197147612294307No Hit
GGCTGCGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT4880.15012566872064012No Hit
GGCTGCGGGGGGCATCTGAATTACAAAACAGTGTAGCTTATGAATGTGTG4770.14674168848308472No Hit
GGCTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA4740.14581878478193325No Hit
GGCTGCGGGGGTGCGTCCTCACTCTGAGTACCCCTCTGTCTACGCAGCAC4310.13259049839876208No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC4300.1322828638317116No Hit
GGCTGCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA4240.13043705642940862No Hit
GGCTGCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC4190.12889888359415616No Hit
GGCTGCGGGGAGACCAGTGCCAAGGAGGCCATCAATGTGGAGCAGGCCTT4150.12766834532595422No Hit
GGCTGCGGGGGACACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGT4120.12674544162480272No Hit
GGCTGCGGGGCTGGCTGCGGGAGCAGGACGGTGGCCATGGAAGTCGGAAT4100.12613017249070174No Hit
GGCTGCGGGTCAACGCAAAGTGCAGTGTGGCTGCGGGGCTGGTGAGATGG4090.12582253792365125No Hit
GGCTGCGGGAGTGAGGCATGTGCTGCACGTCCCTGTCGTACTTAGAGACC4070.12520726878955027No Hit
GGCTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG4050.12459199965544929No Hit
GGCTGCGGGGCTGGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA4020.1236690959542978No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT4010.12336146138724731No Hit
GGCTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG3900.1199774811496919No Hit
GGCTGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG3870.11905457744854044No Hit
GGCTGCGGGGAGTGCAGTGCTGGCTGCGGGAACACAAGTCATCCATATTA3770.1159782317780355No Hit
GGCTGCGGGGTTTTAATAGATGAGGAGACGGGTTTTATCAAGGTTGGTCG3760.11567059721098502No Hit
GGCTGCGGGGCTCTTGCTTCTTGCTCTCTTGCTTCTTGCACTCTGCTCCT3660.11259425154048008No Hit
GGCTGCGGGGTGCCTCCCCTCCCTATCCCCAGTCCCTGTGTGGTGTCTGC3620.11136371327227813No Hit
GGCTGCTGCTGGATCCCGCGCTCCTAGCATTGTGCCTGCCTCAAAGTGGG3610.11105607870522763No Hit
GGCTGCGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAGCACTGACTGC3550.10921027130292467No Hit
GGCTGCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCT3470.10674919476652074No Hit
GGCTGCGGGCTGGCTGCGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGAC3420.10521102193126827No Hit
GGCTGCGGGGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCG3410.1049033873642178No Hit
GGCTGCGGGGAAAGGTTATTGCTATTATTGTTACCTAAAAACAAAGAAAG3310.10182704169371286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGA454.783942E-1044.00000437
CTACGCA454.783942E-1044.00000440
AATAGGT454.783942E-1044.00000416
GTGCCAA454.783942E-1044.00000417
TCACGAC408.28004E-944.015
CTTGTAT254.435774E-544.040
GTGCATA207.8481424E-444.036
AAACTCT207.8481424E-444.039
AGACTAC207.8481424E-444.025
AACCGAA302.522096E-644.044
AATCCGG207.8481424E-444.037
CTCACGA408.28004E-944.014
GGTACGC351.4418038E-744.09
GCCTTAA254.435774E-544.029
GGTACAC254.435774E-544.036
AAATCGG302.522096E-644.036
AAATCCG207.8481424E-444.028
CCGGGAT254.435774E-544.042
CGAACGT502.7284841E-1144.037
AATTTCG207.8481424E-444.029
Produced by FastQC (version 0.11.5)