##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	325061
Sequences flagged as poor quality	0
Sequence length	50
%GC	61
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21685775900523	31.0	31.0	34.0	31.0	34.0
2	32.881649905710006	34.0	31.0	34.0	31.0	34.0
3	32.951470647047785	34.0	31.0	34.0	31.0	34.0
4	36.35570246815213	37.0	37.0	37.0	35.0	37.0
5	36.3112738839787	37.0	37.0	37.0	35.0	37.0
6	35.831702357403685	37.0	35.0	37.0	35.0	37.0
7	35.98458135549943	37.0	35.0	37.0	35.0	37.0
8	36.45098612260468	37.0	37.0	37.0	35.0	37.0
9	38.667597773956274	39.0	39.0	39.0	38.0	39.0
10	37.9304315190072	39.0	38.0	39.0	35.0	39.0
11	37.17869261461695	39.0	37.0	39.0	34.0	39.0
12	36.46615558310594	38.0	35.0	39.0	32.0	39.0
13	36.38981914163803	37.0	35.0	39.0	32.0	39.0
14	37.27398242176084	39.0	36.0	40.0	33.0	40.0
15	37.05559571895736	38.0	36.0	40.0	33.0	40.0
16	36.90528239315083	38.0	36.0	40.0	32.0	40.0
17	36.7131430716081	38.0	35.0	40.0	32.0	40.0
18	36.261092533401424	38.0	35.0	40.0	31.0	40.0
19	36.269167940786495	38.0	35.0	40.0	31.0	40.0
20	36.315688440015876	38.0	35.0	40.0	31.0	40.0
21	36.234122210908104	38.0	35.0	40.0	30.0	40.0
22	35.633201153014355	38.0	34.0	39.0	29.0	40.0
23	34.879994831739275	37.0	33.0	39.0	27.0	40.0
24	34.73368075530439	37.0	33.0	39.0	27.0	40.0
25	34.76408735591166	36.0	33.0	39.0	28.0	40.0
26	34.54383638763186	36.0	33.0	39.0	28.0	40.0
27	34.27636658965548	36.0	32.0	39.0	27.0	40.0
28	34.000753704689274	35.0	32.0	38.0	26.0	40.0
29	34.54174139623024	36.0	33.0	39.0	27.0	40.0
30	34.8819421585487	36.0	33.0	39.0	28.0	40.0
31	34.95149218146748	36.0	33.0	39.0	29.0	40.0
32	34.912997868092454	36.0	33.0	39.0	29.0	40.0
33	34.76025730555188	35.0	33.0	39.0	29.0	40.0
34	34.310215621068046	35.0	33.0	39.0	26.0	40.0
35	34.27523141810306	35.0	33.0	39.0	27.0	40.0
36	34.18259342092715	35.0	33.0	39.0	27.0	40.0
37	34.08211997132846	35.0	33.0	39.0	26.0	40.0
38	34.00524209302254	35.0	33.0	39.0	26.0	40.0
39	33.86386247504315	35.0	33.0	38.0	26.0	40.0
40	33.6807891441914	35.0	33.0	38.0	26.0	40.0
41	33.476839731619606	35.0	32.0	38.0	24.0	40.0
42	33.41097824715976	35.0	32.0	38.0	25.0	40.0
43	33.220927764327314	35.0	32.0	38.0	24.0	40.0
44	32.989900357163734	35.0	32.0	37.0	23.0	40.0
45	32.92152857463676	35.0	32.0	37.0	23.0	40.0
46	32.815637680312314	35.0	32.0	37.0	23.0	40.0
47	32.660611392938556	34.0	31.0	36.0	23.0	40.0
48	32.67524864563882	34.0	31.0	36.0	23.0	40.0
49	32.6576027268728	34.0	31.0	36.0	24.0	40.0
50	31.939429830093427	34.0	30.0	35.0	23.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	4.0
16	12.0
17	32.0
18	66.0
19	142.0
20	237.0
21	511.0
22	881.0
23	1275.0
24	1759.0
25	2250.0
26	3105.0
27	4302.0
28	5703.0
29	7301.0
30	10158.0
31	14168.0
32	19998.0
33	28370.0
34	40618.0
35	42843.0
36	46410.0
37	46858.0
38	45798.0
39	2257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.89466900058758	0.5106733813038169	0.4955992875183427	0.09905833059025845
8	99.29151759208271	0.3897729964529734	0.23072592528786906	0.08798348617644072
9	97.83855953190324	1.0016581503164022	0.4995985368899991	0.660183780890356
10	63.674510322677904	25.035916335703146	4.12045739107429	7.1691159505446675
11	31.961078074576772	21.097270973755695	19.670154217208463	27.27149673445907
12	27.243809623424525	15.828721378448968	34.246802907761925	22.68066609036458
13	33.12947416023454	15.728124875023457	26.29444934950671	24.847951615235296
14	32.94735449654065	15.885633773353309	26.476569013200603	24.69044271690544
15	24.895634973128118	15.803495343950827	25.788082852141596	33.512786830779454
16	27.944601167165544	17.677912760989475	31.273514817218924	23.103971254626053
17	37.739993416620266	17.57454754646051	20.239585800818922	24.4458732361003
18	29.37817824962084	17.249685443655192	21.05051051956402	32.321625787159945
19	37.275772854941074	18.436539603335987	19.949486404090308	24.338201137632627
20	37.326840193071455	17.85480263704351	20.884080218789705	23.93427695109533
21	38.25558895099689	18.15536160905184	22.077087069811512	21.51196237013976
22	35.30906506778727	20.13652822085701	22.0361716723938	22.51823503896192
23	29.926075413537767	22.302583207459524	23.43775475987584	24.33358661912687
24	31.1679961607206	19.154866317398888	24.104706501241306	25.572431020639204
25	29.55968264418063	20.427242886719725	24.364965344966024	25.648109124133622
26	28.435585936178132	19.95748490283362	22.867400272564225	28.739528888424022
27	27.023543273416372	20.111302186358866	24.21422440711128	28.650930133113476
28	29.058853569022432	21.14464669708147	23.95581137078887	25.840688363107233
29	30.04820633665681	20.49984464454364	22.720966218648194	26.730982800151352
30	28.48634563974146	21.198790380882357	23.75215728740144	26.562706691974736
31	32.402533678294226	22.228135642233305	20.121761761638584	25.247568917833885
32	32.94150943976669	20.909613887854896	22.4748585650078	23.674018107370618
33	32.41606959924445	21.691005688163145	22.684972974303285	23.207951738289122
34	29.263430556111008	22.827407778847665	25.020226972783572	22.888934692257763
35	28.599862794983093	24.342508021571334	24.81441944742679	22.24320973601878
36	29.81286589286319	24.12624092093484	22.495777715567232	23.565115470634744
37	30.499813881086936	25.04268429617826	21.51442344667616	22.943078376058647
38	29.60921180947576	23.354693426772204	22.639750692946862	24.396344070805174
39	29.690734969744142	23.847216368620046	21.907272788799638	24.554775872836178
40	29.21020977601127	24.063175834689492	21.555031209526827	25.171583179772412
41	26.596546494350292	25.167891564967803	22.266282328547565	25.96927961213434
42	27.58190001261302	21.71961570289884	22.64436521145262	28.054119073035523
43	25.79669662001901	22.680973724931626	25.25433687830899	26.267992776740368
44	27.019544024044716	21.717769895496538	26.033575236647895	25.229110843810854
45	28.04612057429221	22.559150436379632	24.011800861992057	25.3829281273361
46	28.064271013748186	22.04447780570416	23.085205546035976	26.806045634511676
47	26.01265608608846	23.523277169515875	24.865794420124224	25.59827232427144
48	27.213353801286527	22.88708888485546	24.21330150341013	25.686255810447882
49	28.31560845502844	22.067550398232946	23.335620083615076	26.28122106312354
50	25.789928659543904	22.90124007493978	24.86610205469127	26.442729210825043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	22.5
12	44.0
13	38.5
14	33.0
15	187.0
16	341.0
17	471.0
18	601.0
19	654.5
20	708.0
21	585.0
22	462.0
23	445.0
24	428.0
25	561.0
26	694.0
27	630.0
28	566.0
29	722.5
30	879.0
31	1050.5
32	1222.0
33	1537.0
34	1852.0
35	2694.5
36	3537.0
37	3518.5
38	3500.0
39	3496.5
40	3493.0
41	4391.5
42	5290.0
43	5892.0
44	6494.0
45	6817.5
46	7141.0
47	7298.5
48	7456.0
49	8047.0
50	8638.0
51	9694.5
52	10751.0
53	11406.5
54	12062.0
55	13593.5
56	15125.0
57	16800.5
58	18476.0
59	17123.0
60	15770.0
61	20512.0
62	25254.0
63	26520.5
64	27787.0
65	29005.0
66	30223.0
67	29111.0
68	27999.0
69	27283.0
70	26567.0
71	23123.5
72	19680.0
73	16357.5
74	13035.0
75	12020.0
76	11005.0
77	9594.0
78	8183.0
79	7140.5
80	6098.0
81	4181.5
82	2265.0
83	1632.0
84	999.0
85	673.5
86	348.0
87	192.0
88	36.0
89	24.0
90	12.0
91	8.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	325061.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.624467407655795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.85753995830439	7.421991564660171
2	8.87190178364605	1.8851846268854153
3	3.2690525828121384	1.0419582786000166
4	1.5693768820940468	0.6669517413654668
5	0.952629140606903	0.5060588627980595
6	0.6428075052119527	0.40976924331125547
7	0.41985174889969884	0.3122490855562494
8	0.3880009265693769	0.3297842558781275
9	0.2750752837618717	0.2630275548281707
>10	8.121959694232107	25.257720858546552
>50	3.8684271484827426	28.3842109634807
>100	1.7257354644429002	30.280162800212885
>500	0.031850822330321986	2.3051058109093367
>1k	0.0057910586055130876	0.9358243529675968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1704	0.5242093022540385	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1338	0.41161505071355836	No Hit
GGCTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	917	0.28210089798530125	No Hit
GGCTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	883	0.2716413227055845	No Hit
GGCTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	829	0.25502905608485793	No Hit
GGCTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	705	0.2168823697705969	No Hit
GGCTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	642	0.19750139204641592	No Hit
GGCTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	634	0.19504031551001197	No Hit
GGCTGCGGGACGTACTTATTTCTCTGCTTGACGGGATGGGATGTTACCTG	613	0.18857998960195163	No Hit
GGCTGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	609	0.18734945133374967	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	565	0.173813530383528	No Hit
GGCTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	551	0.16950664644482114	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCT	545	0.1676608390425182	No Hit
GGCTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	494	0.15197147612294307	No Hit
GGCTGCGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCT	488	0.15012566872064012	No Hit
GGCTGCGGGGGGCATCTGAATTACAAAACAGTGTAGCTTATGAATGTGTG	477	0.14674168848308472	No Hit
GGCTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA	474	0.14581878478193325	No Hit
GGCTGCGGGGGTGCGTCCTCACTCTGAGTACCCCTCTGTCTACGCAGCAC	431	0.13259049839876208	No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	430	0.1322828638317116	No Hit
GGCTGCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	424	0.13043705642940862	No Hit
GGCTGCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC	419	0.12889888359415616	No Hit
GGCTGCGGGGAGACCAGTGCCAAGGAGGCCATCAATGTGGAGCAGGCCTT	415	0.12766834532595422	No Hit
GGCTGCGGGGGACACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGT	412	0.12674544162480272	No Hit
GGCTGCGGGGCTGGCTGCGGGAGCAGGACGGTGGCCATGGAAGTCGGAAT	410	0.12613017249070174	No Hit
GGCTGCGGGTCAACGCAAAGTGCAGTGTGGCTGCGGGGCTGGTGAGATGG	409	0.12582253792365125	No Hit
GGCTGCGGGAGTGAGGCATGTGCTGCACGTCCCTGTCGTACTTAGAGACC	407	0.12520726878955027	No Hit
GGCTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	405	0.12459199965544929	No Hit
GGCTGCGGGGCTGGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	402	0.1236690959542978	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	401	0.12336146138724731	No Hit
GGCTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	390	0.1199774811496919	No Hit
GGCTGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	387	0.11905457744854044	No Hit
GGCTGCGGGGAGTGCAGTGCTGGCTGCGGGAACACAAGTCATCCATATTA	377	0.1159782317780355	No Hit
GGCTGCGGGGTTTTAATAGATGAGGAGACGGGTTTTATCAAGGTTGGTCG	376	0.11567059721098502	No Hit
GGCTGCGGGGCTCTTGCTTCTTGCTCTCTTGCTTCTTGCACTCTGCTCCT	366	0.11259425154048008	No Hit
GGCTGCGGGGTGCCTCCCCTCCCTATCCCCAGTCCCTGTGTGGTGTCTGC	362	0.11136371327227813	No Hit
GGCTGCTGCTGGATCCCGCGCTCCTAGCATTGTGCCTGCCTCAAAGTGGG	361	0.11105607870522763	No Hit
GGCTGCGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	355	0.10921027130292467	No Hit
GGCTGCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCT	347	0.10674919476652074	No Hit
GGCTGCGGGCTGGCTGCGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGAC	342	0.10521102193126827	No Hit
GGCTGCGGGGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCG	341	0.1049033873642178	No Hit
GGCTGCGGGGAAAGGTTATTGCTATTATTGTTACCTAAAAACAAAGAAAG	331	0.10182704169371286	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.0763456705049205E-4	0.0	0.0	0.0	0.0
9	9.229037011514762E-4	0.0	0.0	0.0	0.0
10	0.006152691341009841	0.0	0.0	0.0	0.0
11	0.009844306145615746	0.0	0.0	0.0	0.0
12	0.015381728352524602	0.0	0.0	0.0	0.0
13	0.02553366906519084	0.0	0.0	0.0	0.0
14	0.03137872583915019	0.0	0.0	0.0	0.0
15	0.03937722458246298	0.0	0.0	0.0	0.0
16	0.047375723325775776	0.0	0.0	0.0	0.0
17	0.05660476033729054	0.0	0.0	0.0	0.0
18	0.06644906648290629	0.0	0.0	0.0	0.0
19	0.07844681459787547	0.0	0.0	0.0	0.0
20	0.08798348617644072	0.0	0.0	0.0	0.0
21	0.09875069602320795	0.0	0.0	0.0	0.0
22	0.10767209846767223	0.0	0.0	0.0	0.0
23	0.11905457744854042	0.0	0.0	0.0	0.0
24	0.1279759798930047	0.0	0.0	0.0	0.0
25	0.13628211320336797	0.0	0.0	0.0	0.0
26	0.14674168848308472	0.0	0.0	0.0	0.0
27	0.1581241674639529	0.0	0.0	0.0	0.0
28	0.17166008841417457	0.0	0.0	0.0	0.0
29	0.18119675999273982	0.0	0.0	0.0	0.0
30	0.19350214267475951	0.0	0.0	0.0	0.0
31	0.20580752535677918	0.0	0.0	0.0	0.0
32	0.21872817717289986	0.0	0.0	0.0	0.0
33	0.22549613764801069	0.0	0.0	0.0	0.0
34	0.23780152033003035	0.0	0.0	0.0	0.0
35	0.2479534610426966	0.0	0.0	0.0	0.0
36	0.26118174742586775	0.0	0.0	0.0	0.0
37	0.2731794955408369	0.0	0.0	0.0	0.0
38	0.28517724365580616	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATGA	45	4.783942E-10	44.000004	37
CTACGCA	45	4.783942E-10	44.000004	40
AATAGGT	45	4.783942E-10	44.000004	16
GTGCCAA	45	4.783942E-10	44.000004	17
TCACGAC	40	8.28004E-9	44.0	15
CTTGTAT	25	4.435774E-5	44.0	40
GTGCATA	20	7.8481424E-4	44.0	36
AAACTCT	20	7.8481424E-4	44.0	39
AGACTAC	20	7.8481424E-4	44.0	25
AACCGAA	30	2.522096E-6	44.0	44
AATCCGG	20	7.8481424E-4	44.0	37
CTCACGA	40	8.28004E-9	44.0	14
GGTACGC	35	1.4418038E-7	44.0	9
GCCTTAA	25	4.435774E-5	44.0	29
GGTACAC	25	4.435774E-5	44.0	36
AAATCGG	30	2.522096E-6	44.0	36
AAATCCG	20	7.8481424E-4	44.0	28
CCGGGAT	25	4.435774E-5	44.0	42
CGAACGT	50	2.7284841E-11	44.0	37
AATTTCG	20	7.8481424E-4	44.0	29
>>END_MODULE