FastQCFastQC Report
Fri 17 Jun 2016
SRR1527782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491921
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24750.5031295675525135No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19960.4057562088221483No Hit
GGCTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC14470.29415292292868167No Hit
GGCTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12980.26386350653865154No Hit
GGCTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT12580.2557321195883079No Hit
GGCTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11440.23255766677982848No Hit
GGCTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10030.2038945277798671No Hit
GGCTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC9370.19047773931180007No Hit
GGCTGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG9060.18417591442528375No Hit
GGCTGCGGGACGTACTTATTTCTCTGCTTGACGGGATGGGATGTTACCTGCT8820.17929708225507754No Hit
GGCTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT8730.17746752019125023No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCT8450.17177554932600966No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT8360.16994598726218235No Hit
GGCTGCGGGGGGCATCTGAATTACAAAACAGTGTAGCTTATGAATGTGTGCA8160.16588029378701052No Hit
GGCTGCGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC7540.15327664401397786No Hit
GGCTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT7160.14555182641115139No Hit
GGCTGCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA7060.14351897967356547No Hit
GGCTGCGGGGCTGGCTGCGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCC6970.14168941760973813No Hit
GGCTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG6810.1384368628296007No Hit
GGCTGCGGGTCAACGCAAAGTGCAGTGTGGCTGCGGGGCTGGTGAGATGGCT6790.1380302934820835No Hit
GGCTGCGGGGGTGCGTCCTCACTCTGAGTACCCCTCTGTCTACGCAGCACTG6610.13437116935442886No Hit
GGCTGCGGGGGACACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGG6600.13416788468067026No Hit
GGCTGCGGGGAGTGCAGTGCTGGCTGCGGGAACACAAGTCATCCATATTAAT6570.1335580306593945No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT6550.13315146131187733No Hit
GGCTGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC6390.12989890653173986No Hit
GGCTGCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC6220.1264430670778438No Hit
GGCTGCGGGGAGACCAGTGCCAAGGAGGCCATCAATGTGGAGCAGGCCTTCC6100.1240036509927407No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC5990.1217675195813962No Hit
GGCTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5860.1191248188225345No Hit
GGCTGCGGGGCTGGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC5730.11648211806367284No Hit
GGCTGCGGGGTGCCTCCCCTCCCTATCCCCAGTCCCTGTGTGGTGTCTGCCA5530.112416424588501No Hit
GGCTGCGGGGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGCC5400.10977372382963932No Hit
GGCTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT5380.10936715448212214No Hit
GGCTGCGGGGTTTTAATAGATGAGGAGACGGGTTTTATCAAGGTTGGTCGGC5310.107944161765812No Hit
GGCTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA5300.10774087709205339No Hit
GGCTGCGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC5270.10713102307077763No Hit
GGCTGCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG5230.10631788437574326No Hit
GGCTGCGGGGAAAGGTTATTGCTATTATTGTTACCTAAAAACAAAGAAAGAA5230.10631788437574326No Hit
GGCTGCGGGACTGTGGATCCTCCAGCTCCATCAGCTGGCCGTGGCAGAGGCC5180.1053014610069503No Hit
GGCTGCGGGCTGGCTGCGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCA5110.10387846829064017No Hit
GGCTGCTGCTGGATCCCGCGCTCCTAGCATTGTGCCTGCCTCAAAGTGGGCA5110.10387846829064017No Hit
GGCTGCGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT5040.10245547557433002No Hit
GGCTGCGGGGCTCTTGCTTCTTGCTCTCTTGCTTCTTGCACTCTGCTCCTGA5030.10225219090057144No Hit
GGCTGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG5020.10204890622681284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAT750.046.00000434
CCGCATT750.046.00000435
AACGTTT206.307917E-446.010
CGTGGTA206.307917E-446.042
AACAATG206.307917E-446.026
AGCGTCG206.307917E-446.024
CCCCTTA206.307917E-446.019
AACCGAC301.8592164E-646.043
CTGTATT206.307917E-446.012
GTCGATA206.307917E-446.027
GGTAATC253.414286E-546.010
GTATTAC206.307917E-446.014
AATCAAC206.307917E-446.033
GATCGCC206.307917E-446.032
GTACACG206.307917E-446.037
AGATATG206.307917E-446.041
TAAAGTT453.092282E-1046.036
GATCGAC206.307917E-446.09
CGACGTA301.8592164E-646.010
CCGATTG501.6370905E-1146.042
Produced by FastQC (version 0.11.5)