FastQCFastQC Report
Fri 17 Jun 2016
SRR1527781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497604
Sequences flagged as poor quality0
Sequence length52
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24720.49678057250343644No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC19270.38725572945555103No Hit
GGCTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC13990.28114725765870047No Hit
GGCTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12430.24979702735508555No Hit
GGCTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11640.23392094918851133No Hit
GGCTGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT11620.23351902315897782No Hit
GGCTGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9660.1941302722646924No Hit
GGCTGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC8920.179259009171952No Hit
GGCTGCGGGACGTACTTATTTCTCTGCTTGACGGGATGGGATGTTACCTGCT8830.17745034203905113No Hit
GGCTGCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG8820.1772493790242844No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT8600.1728281926994156No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCGCCAACTGCTCT8150.1637848570349113No Hit
GGCTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT8010.1609713748281766No Hit
GGCTGCGGGGGGCATCTGAATTACAAAACAGTGTAGCTTATGAATGTGTGCA7720.15514344739994052No Hit
GGCTGCGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTC7480.1503203350455382No Hit
GGCTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6820.13705677607093192No Hit
GGCTGCGGGGAGTGCAGTGCTGGCTGCGGGAACACAAGTCATCCATATTAAT6730.13524810893803105No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT6660.1338413678346637No Hit
GGCTGCGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG6530.1312288486426958No Hit
GGCTGCGGGGGACACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGG6340.1274105513621273No Hit
GGCTGCGGGGAGACCAGTGCCAAGGAGGCCATCAATGTGGAGCAGGCCTTCC6280.12620477327352675No Hit
GGCTGCGGGGCTGGCTGCGGGAGCAGGACGGTGGCCATGGAAGTCGGAATCC6220.12499899518492617No Hit
GGCTGCGGGTCAACGCAAAGTGCAGTGTGGCTGCGGGGCTGGTGAGATGGCT6190.12439610614062588No Hit
GGCTGCGGGGGTGCGTCCTCACTCTGAGTACCCCTCTGTCTACGCAGCACTG6180.12419514312585911No Hit
GGCTGCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC6120.12298936503725853No Hit
GGCTGCGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA6000.1205778088600574No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC5650.11354410334322071No Hit
GGCTGCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC5530.11113254716601957No Hit
GGCTGCGGGGAAAGGTTATTGCTATTATTGTTACCTAAAAACAAAGAAAGAA5510.11073062113648605No Hit
GGCTGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5480.11012773209218575No Hit
GGCTGCGGGGCTGGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC5440.1093238800331187No Hit
GGCTGCGGGGTGCCTCCCCTCCCTATCCCCAGTCCCTGTGTGGTGTCTGCCA5390.10831906495928488No Hit
GGCTGCGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG5320.10691232385591756No Hit
GGCTGCGGGACTGTGGATCCTCCAGCTCCATCAGCTGGCCGTGGCAGAGGCC5180.1040988416491829No Hit
GGCTGCGGGGTTTTAATAGATGAGGAGACGGGTTTTATCAAGGTTGGTCGGC5150.1034959526048826No Hit
GGCTGCGGGGGGGCTGGGGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC5110.10269210054581555No Hit
GGCTGCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA5040.10128535944244821No Hit
GGCTGCTGCTGGATCCCGCGCTCCTAGCATTGTGCCTGCCTCAAAGTGGGCA5030.10108439642768144No Hit
GGCTGCGGGGCTCTTGCTTCTTGCTCTCTTGCTTCTTGCACTCTGCTCCTGA5020.10088343341291468No Hit
GGCTGCGGGGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGCC5020.10088343341291468No Hit
GGCTGCGGGGGTTCGGAGCCGCCGCGATGGCGGAGGACAGCGAGTCTGCGGC5000.10048150738338117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGG351.0179974E-746.00000428
ACACGTA351.0179974E-746.00000423
CGAAATG351.0179974E-746.00000446
CGACGGA351.0179974E-746.00000428
GCGGTAA351.0179974E-746.00000441
GACCGTA351.0179974E-746.00000427
CGACCGA351.0179974E-746.00000437
GATCATA351.0179974E-746.00000445
GTACTAC351.0179974E-746.00000435
TGCTCGA351.0179974E-746.00000442
TCTCACG351.0179974E-746.00000427
TCGCGTG351.0179974E-746.00000444
AGTGTAA551.8189894E-1246.00000440
TCACGCG600.046.023
TCACGAC600.046.015
CTTGTAT301.85926E-646.040
TTTAGGC206.3079887E-446.023
TGTTTCG253.4143435E-546.043
AACAATG253.4143435E-546.026
CGGAATT301.85926E-646.010
Produced by FastQC (version 0.11.5)