FastQCFastQC Report
Fri 17 Jun 2016
SRR1527780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143347
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGA53860.47107308629838535No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC49010.4286537682785716No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG25240.22075537872579365No Hit
TAGCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG24830.21716941575917023No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24750.21646971566812176No Hit
TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGAC24350.21297121521287937No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA23690.20719868946172948No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA19750.1727384599775921No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG18880.16512922148743994No Hit
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC17880.15638297034933402No Hit
TAGCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT15480.1353919676178798No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC14970.13093137953744577No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG14640.12804511666187082No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13990.12236005342210195No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTG11980.10478008863450904No Hit
TAGCATGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG11670.10206875078169621No Hit
TAGCATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC11590.10136905069064771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACT207.8564626E-444.00000437
TAAAGCG207.8564626E-444.00000419
GCGCGTA207.8564626E-444.00000434
ATTCGGC207.8564626E-444.00000411
TACGGTA207.8564626E-444.00000430
TCGATTA408.312782E-944.00000435
CGCGAAC207.8564626E-444.00000436
ACTACCG207.8564626E-444.00000435
CGTACCG207.8564626E-444.00000423
CACGTAT207.8564626E-444.00000429
TATCGCC207.8564626E-444.00000414
TACCGAC207.8564626E-444.00000425
TAATCGG207.8564626E-444.00000423
ACAACGG302.527704E-644.038
TCGTTGA254.442825E-544.024
GATACGC254.442825E-544.09
GATCGAT351.4460784E-744.09
TATGCGT254.442825E-544.038
CTTATCG302.527704E-644.030
CGGATAC302.527704E-644.038
Produced by FastQC (version 0.11.5)