FastQCFastQC Report
Fri 17 Jun 2016
SRR1527779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721027
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC79370.4611781221328892No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA72650.42213166905574406No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC38650.22457521003447362No Hit
TAGCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG38300.22254154060337228No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC37910.22027545180871655No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC35150.20423851572346047No Hit
TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT34300.1992996042479287No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA28410.16507585296453803No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT27760.16129903830677844No Hit
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT25520.14828355394773005No Hit
TAGCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC25120.14595936031218568No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG23240.1350356502251272No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC22420.13027105327226127No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG21800.12666855313716752No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCT18590.10801689921192403No Hit
TAGCATGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG17780.10331040709994671No Hit
TAGCATGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT17630.10243883448661757No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT206.312334E-446.043
CCGATAG206.312334E-446.022
ATAATCG301.8619467E-646.043
ATTACCG253.4178694E-546.044
GCACGTA206.312334E-446.09
CGTCTAA253.4178694E-546.023
ATCCGTA253.4178694E-546.040
ATCGTAC206.312334E-446.043
CGTAGTA206.312334E-446.038
CGGATAC405.6134013E-946.038
CGTAGAC253.4178694E-546.035
TAACACC253.4178694E-546.021
CGTACAT206.312334E-446.024
CGTAACC253.4178694E-546.018
TTACGCC253.4178694E-546.019
CACGTAC405.6134013E-946.033
GCGTCTA253.4178694E-546.019
TACCGAC405.6134013E-946.025
TATCCCG206.312334E-446.044
TAGCATG1761850.044.711521
Produced by FastQC (version 0.11.5)