FastQCFastQC Report
Fri 17 Jun 2016
SRR1527778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733593
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC76590.44179919969681464No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA69500.40090148033592665No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36470.21037233076044953No Hit
TAGCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG36140.20846876977468182No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC35220.2031618724810264No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34050.19641288353148637No Hit
TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT33750.1946823735444248No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA27950.16122584712790142No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT26840.15482296017577366No Hit
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24080.13890226829480737No Hit
TAGCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC23390.13492209532456578No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG22100.12748090238020113No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG21650.12488513739960878No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC20370.1175016281214795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA206.3123455E-446.00000445
ACCGATA206.3123455E-446.00000417
GTCGTAC206.3123455E-446.00000416
TTGCGAC206.3123455E-446.00000421
ACGCTTA405.6134013E-946.00000438
TCGACAC206.3123455E-446.00000413
GCGTATA206.3123455E-446.00000416
CCGTTCG206.3123455E-446.00000445
TGCGTAT405.6134013E-946.00000418
GTACGAC206.3123455E-446.00000415
CGTATAG501.6370905E-1146.017
TCACACG253.4178796E-546.039
TAACGGA301.8619558E-646.012
CTCGATT301.8619558E-646.034
ACGAATC253.4178796E-546.013
TAATCGT301.8619558E-646.021
TAGCATG1773400.044.736781
AGCATGG1763600.044.6997642
GCATGGG1727950.044.6369973
ATGGGAT218850.044.4866335
Produced by FastQC (version 0.11.5)