##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1733593
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42850426830288	33.0	31.0	34.0	31.0	34.0
2	32.74070153721202	34.0	31.0	34.0	31.0	34.0
3	32.826863629467816	34.0	31.0	34.0	31.0	34.0
4	36.216223185026706	37.0	37.0	37.0	35.0	37.0
5	36.12704019917016	37.0	37.0	37.0	35.0	37.0
6	36.2328481944724	37.0	37.0	37.0	35.0	37.0
7	36.52763422556505	37.0	37.0	37.0	35.0	37.0
8	36.643022900992335	37.0	37.0	37.0	35.0	37.0
9	38.667286958357586	39.0	39.0	39.0	38.0	39.0
10	38.04121555636185	39.0	38.0	39.0	37.0	39.0
11	37.80822719058049	39.0	38.0	39.0	35.0	39.0
12	37.41360226996763	39.0	37.0	39.0	35.0	39.0
13	37.14059124604218	39.0	37.0	39.0	34.0	39.0
14	38.056167162650056	40.0	38.0	40.0	34.0	40.0
15	38.15051052928801	40.0	38.0	40.0	34.0	40.0
16	38.23686528498904	40.0	38.0	40.0	34.0	40.0
17	38.24468084492727	40.0	38.0	40.0	35.0	40.0
18	38.23603060233861	40.0	38.0	40.0	34.0	40.0
19	38.19292417539757	40.0	38.0	40.0	34.0	40.0
20	38.15951494958736	40.0	38.0	40.0	34.0	40.0
21	38.192305806495526	40.0	38.0	40.0	34.0	40.0
22	38.07185423568277	40.0	38.0	40.0	34.0	40.0
23	38.07634606277252	40.0	38.0	40.0	34.0	40.0
24	38.073460148950765	40.0	38.0	40.0	34.0	40.0
25	38.00763847108289	40.0	38.0	40.0	34.0	40.0
26	37.846308216519105	40.0	38.0	40.0	34.0	40.0
27	37.74657488810811	40.0	37.0	40.0	34.0	40.0
28	37.66176432415221	40.0	37.0	40.0	33.0	40.0
29	37.59783063268022	40.0	37.0	40.0	33.0	40.0
30	37.51525761813759	40.0	37.0	40.0	33.0	40.0
31	37.42406377967608	40.0	36.0	40.0	33.0	40.0
32	37.34029152171242	40.0	36.0	40.0	33.0	40.0
33	37.140706036537985	40.0	36.0	40.0	32.0	40.0
34	37.10887445899932	40.0	36.0	40.0	32.0	40.0
35	37.06105931438348	40.0	36.0	40.0	32.0	40.0
36	36.87716609377172	39.0	35.0	40.0	32.0	40.0
37	36.84636936120531	39.0	35.0	40.0	31.0	40.0
38	36.52970045448961	39.0	35.0	40.0	31.0	40.0
39	36.599295220965935	39.0	35.0	40.0	31.0	40.0
40	36.42333235078822	39.0	35.0	40.0	31.0	40.0
41	36.087017541026064	39.0	35.0	40.0	30.0	40.0
42	36.202989975155646	39.0	35.0	40.0	30.0	40.0
43	36.16496951706658	39.0	35.0	40.0	30.0	40.0
44	36.027686429283	38.0	35.0	40.0	30.0	40.0
45	35.929180609289496	38.0	35.0	40.0	30.0	40.0
46	35.65214845699077	38.0	35.0	40.0	29.0	40.0
47	35.39492372200395	38.0	34.0	40.0	28.0	40.0
48	35.38745599457312	38.0	34.0	40.0	28.0	40.0
49	35.29940014755482	37.0	34.0	40.0	28.0	40.0
50	35.174624609121054	37.0	34.0	40.0	28.0	40.0
51	35.445764374913836	37.0	34.0	40.0	29.0	40.0
52	35.19057760385512	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	11.0
16	27.0
17	80.0
18	188.0
19	383.0
20	673.0
21	1223.0
22	2048.0
23	3284.0
24	4821.0
25	6731.0
26	9234.0
27	12479.0
28	16515.0
29	20833.0
30	26807.0
31	34319.0
32	44920.0
33	61384.0
34	100383.0
35	116057.0
36	156737.0
37	264401.0
38	548832.0
39	301220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.48742294183236	0.4188987842013668	0.07268141945658525	0.020996854509680182
8	99.37874691464489	0.5343238003383723	0.03928257670629727	0.04764670831042811
9	97.68302017832329	1.3007089899416993	0.4881768673500643	0.5280939643849508
10	52.22979096016193	36.385414569625055	4.676357138036436	6.708437332176584
11	32.57540841477786	25.78361818489115	24.092621509200836	17.548351891130157
12	29.79932429353372	18.668164903757685	26.593785277167132	24.93872552554146
13	25.058649867644828	19.1192511737184	29.74469786160881	26.077401097027963
14	24.56914627597135	20.266636978806442	30.144734086951203	25.019482658271002
15	24.84902742454544	21.273274638280153	28.646285489154604	25.231412448019807
16	28.45194921760759	22.037352481234063	25.509216984609424	24.001481316548926
17	29.274979767454067	22.651914261305855	23.307258393406062	24.765847577834013
18	28.03074308675681	22.252916341955693	24.97835420424517	24.73798636704232
19	28.745962864409353	22.207807714959625	24.71860465518723	24.327624765443794
20	27.564139910578785	23.129765752399784	25.040421829114447	24.265672507906988
21	29.247176240328614	21.516699709793475	26.319268709552933	22.91685534032498
22	28.34765714905402	21.354550924005807	25.4862012017815	24.811590725158673
23	26.82480836043985	23.03706810075952	26.085015340971037	24.053108197829594
24	26.678637950199384	23.55304849523504	24.279920373467128	25.488393181098445
25	26.410351218538608	25.04930511371469	24.034591740968036	24.505751926778665
26	27.422180407973496	23.784475364171406	23.88040330112085	24.912940926734244
27	25.767870544008886	21.907679599536916	25.781656940239145	26.542792916215053
28	27.037430354183478	23.06648677053957	24.110215027402624	25.78586784787433
29	28.488578345667065	22.854903082788177	23.768843090621615	24.887675480923146
30	27.05231274007221	23.03793335575305	24.925746700638502	24.98400720353624
31	28.42414569048214	22.793181559916313	24.4702764720439	24.312396277557653
32	28.51799701544711	23.59452305125828	24.608717271008825	23.278762662285786
33	29.45708710175918	22.80708332347904	24.188318711485337	23.547510863276443
34	27.804334696782924	23.63230585264246	26.541869977555287	22.021489473019333
35	27.422295775305965	25.611317073846052	24.851796240524738	22.114590910323244
36	28.46135165520396	25.70995614310856	22.763243737140147	23.06544846454733
37	27.7483815405346	25.21387661348425	23.395283668081262	23.64245817789989
38	28.733099406838857	24.020863028404012	23.485847024070814	23.76019054068631
39	27.717290044433728	23.99346328694221	23.69143161053373	24.597815058090337
40	27.870959331284794	24.342334100333815	24.104619711777794	23.682086856603597
41	26.02767777673306	23.84844655002645	25.55651759092244	24.56735808231805
42	26.56419355638838	22.86084450041042	24.691089546392952	25.88387239680825
43	25.64200478428328	22.765666451122033	26.1702141160007	25.42211464859399
44	25.803519049742356	23.284992498239205	25.615470297815	25.296018154203438
45	26.949751181505693	22.515030921329284	24.627752880866502	25.90746501629852
46	26.1514669244742	23.942874711653772	24.313492267216123	25.592166096655905
47	25.97881971143169	23.897246931661584	25.122563369833635	25.00136998707309
48	26.591593297850185	23.92164712247915	25.099028434009597	24.387731145661064
49	26.81817473882278	24.09792840649449	25.069955866226962	24.01394098845577
50	25.488681599429626	24.610043995332237	24.991159978149426	24.91011442708871
51	24.38167436070635	25.09049125140676	24.498714519497945	26.029119868388946
52	25.21560712347131	24.5667812456557	24.203316464706536	26.014295166166452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.0
4	12.0
5	14.5
6	17.0
7	69.5
8	122.0
9	670.0
10	1218.0
11	2184.5
12	3151.0
13	4376.5
14	5221.5
15	4841.0
16	4589.5
17	4338.0
18	4606.0
19	4874.0
20	4777.5
21	4681.0
22	5613.0
23	6545.0
24	7689.5
25	8834.0
26	12020.0
27	15206.0
28	17295.5
29	19385.0
30	21416.5
31	23448.0
32	26286.5
33	29125.0
34	31097.5
35	33070.0
36	37788.5
37	42507.0
38	46262.0
39	50723.0
40	51429.0
41	55553.0
42	59677.0
43	61741.0
44	63805.0
45	67317.5
46	70830.0
47	75497.0
48	80164.0
49	85675.0
50	91186.0
51	94041.5
52	96897.0
53	101172.5
54	105448.0
55	115707.5
56	125967.0
57	131371.5
58	136776.0
59	137151.0
60	137526.0
61	129681.5
62	121837.0
63	115355.0
64	92631.0
65	76389.0
66	61701.5
67	47014.0
68	42075.0
69	37136.0
70	32327.0
71	27518.0
72	23607.0
73	19696.0
74	15132.0
75	10568.0
76	7362.5
77	4157.0
78	2970.5
79	1784.0
80	1442.5
81	1101.0
82	941.0
83	781.0
84	405.0
85	29.0
86	17.5
87	6.0
88	5.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1733593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.373149862889163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.181604091057	6.578226927634323
2	9.760877366177219	1.8298033269292384
3	3.986573516662368	1.1210025303330433
4	1.9935506078419627	0.74743394426226
5	1.1504440191448135	0.5391642100154433
6	0.6610478892555653	0.3717660559523383
7	0.47542191627910574	0.3119340608570202
8	0.3326580529238724	0.24944430265218964
9	0.26639692332388676	0.22472804567946456
>10	4.67577057422887	12.655003435973148
>50	3.9858301071561972	26.877813156758577
>100	2.4430516216265534	39.87174540372432
>500	0.06252782943833957	3.7949453899843415
>1k	0.02296940673245127	3.9533073320020944
>5k	0.0012760781518028484	0.8736818772422031
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	7659	0.44179919969681464	No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6950	0.40090148033592665	No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3647	0.21037233076044953	No Hit
TAGCATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3614	0.20846876977468182	No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3522	0.2031618724810264	No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3405	0.19641288353148637	No Hit
TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCT	3375	0.1946823735444248	No Hit
TAGCATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2795	0.16122584712790142	No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2684	0.15482296017577366	No Hit
TAGCATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2408	0.13890226829480737	No Hit
TAGCATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2339	0.13492209532456578	No Hit
TAGCATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2210	0.12748090238020113	No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2165	0.12488513739960878	No Hit
TAGCATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2037	0.1175016281214795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.011998202576960106	0.0	0.0	0.0	0.0
8	0.01995854851744325	0.0	0.0	0.0	0.0
9	0.024919343813686372	0.0	0.0	0.0	0.0
10	0.045339361661012705	0.0	0.0	0.0	0.0
11	0.07262373579034986	0.0	0.0	0.0	0.0
12	0.08306447937895457	0.0	0.0	0.0	0.0
13	0.09194776397920389	0.0	0.0	0.0	0.0
14	0.1023885075678086	0.0	0.0	0.0	0.0
15	0.11357913881747331	0.0	0.0	0.0	0.0
16	0.1287499430373796	0.0	0.0	0.0	0.0
17	0.1419018189390474	0.0	0.0	0.0	0.0
18	0.1543038071796552	0.0	0.0	0.0	0.0
19	0.1663596934228507	0.0	0.0	0.0	0.0
20	0.17893473266216464	0.0	0.0	0.0	0.0
21	0.190817567906654	0.0	0.0	0.0	0.0
22	0.20189283182384793	0.0	0.0	0.0	0.0
23	0.2136026160696311	0.0	0.0	0.0	0.0
24	0.2264083899738866	0.0	0.0	0.0	0.0
25	0.2399640515392021	0.0	0.0	0.0	0.0
26	0.2545580190967545	0.0	0.0	0.0	0.0
27	0.27047871097772086	0.0	0.0	0.0	0.0
28	0.2870339231872764	0.0	0.0	0.0	0.0
29	0.2985706564343534	0.0	0.0	0.0	0.0
30	0.31201095066719814	0.0	0.0	0.0	0.0
31	0.32423988790909974	0.0	0.0	0.0	0.0
32	0.33721871281206145	0.0	0.0	0.0	0.0
33	0.3481786093967846	0.0	0.0	0.0	0.0
34	0.3605805976373924	0.0	0.0	0.0	0.0
35	0.3781164321729495	0.0	0.0	0.0	0.0
36	0.3914990427395588	0.0	0.0	0.0	0.0
37	0.4051123879711097	0.0	0.0	0.0	0.0
38	0.4183219475390129	0.0	0.0	0.0	0.0
39	0.43135845610820994	0.0	0.0	0.0	0.0
40	0.44335665868517005	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTTA	20	6.3123455E-4	46.000004	45
ACCGATA	20	6.3123455E-4	46.000004	17
GTCGTAC	20	6.3123455E-4	46.000004	16
TTGCGAC	20	6.3123455E-4	46.000004	21
ACGCTTA	40	5.6134013E-9	46.000004	38
TCGACAC	20	6.3123455E-4	46.000004	13
GCGTATA	20	6.3123455E-4	46.000004	16
CCGTTCG	20	6.3123455E-4	46.000004	45
TGCGTAT	40	5.6134013E-9	46.000004	18
GTACGAC	20	6.3123455E-4	46.000004	15
CGTATAG	50	1.6370905E-11	46.0	17
TCACACG	25	3.4178796E-5	46.0	39
TAACGGA	30	1.8619558E-6	46.0	12
CTCGATT	30	1.8619558E-6	46.0	34
ACGAATC	25	3.4178796E-5	46.0	13
TAATCGT	30	1.8619558E-6	46.0	21
TAGCATG	177340	0.0	44.73678	1
AGCATGG	176360	0.0	44.699764	2
GCATGGG	172795	0.0	44.636997	3
ATGGGAT	21885	0.0	44.486633	5
>>END_MODULE