FastQCFastQC Report
Fri 17 Jun 2016
SRR1527777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336999
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC70102.0801248668393675No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG41111.2198849254745563No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT27510.8163228971005848No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT27450.8145424763871703No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC23520.6979249196585153No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA15330.4548974922774252No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT13180.3910990833800694No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG12540.3721079291036472No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC12370.36706340374897256No Hit
CCGCTAGGGGGGCTGGCAAGATGGCTCAGCAGGTAAGAGCACCGACTACT11990.35578740589734686No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG11670.3462918287591358No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG8470.25133605737702486No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA7700.22848732488820442No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT7430.22047543167783878No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC7190.2133537488241805No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG6580.1952528049044656No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT6560.19465933133332738No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT6190.18368007026727082No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG6020.17863554491259617No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT6000.17804207134145797No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC5950.17655838741361252No Hit
CCGCTAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC5760.17092038848779967No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA5650.1676562838465396No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC5560.16498565277641772No Hit
CCGCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC5550.16468891599084864No Hit
CCGCTAGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGG5450.16172154813515766No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC5360.1590509170650358No Hit
CCGCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG5350.15875418027946672No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5300.15727049635162121No Hit
CCGCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5240.15549007563820666No Hit
CCGCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGC5190.15400639171036115No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC5030.1492586031412556No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC4830.14332386742987369No Hit
CCGCTAGGGGCGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCG4750.1409499731453209No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA4700.13946628921747542No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC4520.1341250270772317No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT4410.1308609224359716No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG4380.12997071207926433No Hit
CCGCTAGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCT4310.12789355458028065No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT4300.12759681779471155No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC4110.12195881886889871No Hit
CCGCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC4040.11988166136991504No Hit
CCGCTAGGGGGAGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC4000.11869471422763866No Hit
CCGCTAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCT3880.1151338728008095No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTG3820.11335345208739493No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG3730.11068282101727306No Hit
CCGCTAGGGAGGGCACCGCCAGTGTGTTTGCACCAGGAGACAGTAAAGGA3650.10830892673272027No Hit
CCGCTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT3630.10771545316158208No Hit
CCGCTAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGG3610.1071219795904439No Hit
CCGCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG3560.1056382956625984No Hit
CCGCTAGGGGGGCCCCAGAAAGGACTCTTTCTCCCTGAGTTTTACAGTAA3520.10445134852032202No Hit
CCGCTAGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC3430.10178071745020015No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGC3380.10029703352235465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCC1300.044.0000045
ATTTATA207.848553E-444.025
GTGCATA302.5223726E-644.033
TTGATAC302.5223726E-644.016
ATCCTAG302.5223726E-644.010
AGACTTA302.5223726E-644.044
CGGAATT207.848553E-444.025
CGGAAAT302.5223726E-644.036
TCGTTCT207.848553E-444.023
TCGTTCC207.848553E-444.027
GATACCA302.5223726E-644.09
CTCACGC254.4361208E-544.025
ACCGGAT302.5223726E-644.030
ATTAGTC207.848553E-444.018
GCGACGT207.848553E-444.017
CCCTCGA207.848553E-444.021
TCCGCGC254.4361208E-544.013
ACGCCCA254.4361208E-544.036
GATCGTA207.848553E-444.036
CTCGTAA254.4361208E-544.043
Produced by FastQC (version 0.11.5)