##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336999
Sequences flagged as poor quality	0
Sequence length	50
%GC	60
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.675924854376426	34.0	31.0	34.0	31.0	34.0
2	33.12857308181923	34.0	33.0	34.0	31.0	34.0
3	33.09258781183327	34.0	34.0	34.0	31.0	34.0
4	36.466864886839424	37.0	37.0	37.0	35.0	37.0
5	36.41749085308859	37.0	37.0	37.0	35.0	37.0
6	36.31250241098638	37.0	37.0	37.0	35.0	37.0
7	36.54016480761071	37.0	37.0	37.0	35.0	37.0
8	36.71031664782388	37.0	37.0	37.0	35.0	37.0
9	38.79433470128991	39.0	39.0	39.0	39.0	39.0
10	38.36985272953332	39.0	39.0	39.0	37.0	39.0
11	38.114433573986865	39.0	39.0	39.0	37.0	39.0
12	37.29576645628028	39.0	35.0	39.0	35.0	39.0
13	37.09068572903777	39.0	35.0	39.0	35.0	39.0
14	37.78158392161401	40.0	36.0	40.0	34.0	40.0
15	37.83934670429289	40.0	36.0	40.0	34.0	40.0
16	37.91645079065516	40.0	36.0	40.0	35.0	40.0
17	37.71839382312707	40.0	36.0	40.0	33.0	40.0
18	37.65439660058338	40.0	36.0	40.0	33.0	40.0
19	37.618749610532966	40.0	36.0	40.0	33.0	40.0
20	37.502408019014894	40.0	35.0	40.0	33.0	40.0
21	37.44976097851922	40.0	35.0	40.0	33.0	40.0
22	37.53798082486892	40.0	35.0	40.0	33.0	40.0
23	37.49958011744842	40.0	35.0	40.0	34.0	40.0
24	37.51024780488963	40.0	35.0	40.0	34.0	40.0
25	37.49796290196707	40.0	35.0	40.0	34.0	40.0
26	37.41637215540699	40.0	35.0	40.0	34.0	40.0
27	37.335748770767864	39.0	35.0	40.0	34.0	40.0
28	37.185831411962646	39.0	35.0	40.0	33.0	40.0
29	37.25522330926798	40.0	35.0	40.0	33.0	40.0
30	37.37724444286185	40.0	35.0	40.0	34.0	40.0
31	37.396722245466606	40.0	35.0	40.0	34.0	40.0
32	37.385716278089845	40.0	35.0	40.0	34.0	40.0
33	37.26190285431114	40.0	35.0	40.0	33.0	40.0
34	36.76738506642453	39.0	35.0	40.0	32.0	40.0
35	36.84679776497853	39.0	35.0	40.0	32.0	40.0
36	36.83550396291977	39.0	35.0	40.0	33.0	40.0
37	36.821949026554975	39.0	35.0	40.0	33.0	40.0
38	36.80555135178442	39.0	35.0	40.0	33.0	40.0
39	36.72652441105166	39.0	35.0	40.0	33.0	40.0
40	36.473532562411165	38.0	35.0	40.0	32.0	40.0
41	36.38013169178544	38.0	35.0	40.0	32.0	40.0
42	36.368630173976776	38.0	35.0	40.0	32.0	40.0
43	36.20873058970501	38.0	35.0	40.0	32.0	40.0
44	35.94195235000697	37.0	35.0	40.0	31.0	40.0
45	35.87209160858044	37.0	35.0	40.0	31.0	40.0
46	35.69465191291369	37.0	35.0	40.0	31.0	40.0
47	35.60701960539942	36.0	35.0	40.0	31.0	40.0
48	35.484743278169965	36.0	35.0	40.0	31.0	40.0
49	35.35282300540951	36.0	35.0	40.0	30.0	40.0
50	34.898079816260584	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	8.0
17	23.0
18	59.0
19	109.0
20	165.0
21	236.0
22	380.0
23	547.0
24	816.0
25	1169.0
26	1515.0
27	1903.0
28	2578.0
29	3299.0
30	4294.0
31	5895.0
32	7725.0
33	11201.0
34	28383.0
35	37442.0
36	25408.0
37	45208.0
38	94407.0
39	64226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.62789207089635	0.2943628912845439	0.041839886765242626	0.03590515105386069
8	99.78664625117581	0.11335345208739493	0.08694387817174531	0.013056418565040253
9	99.6830851130122	0.17092038848779967	0.04273009712194992	0.10326440137804563
10	72.5370104955801	21.828551420033886	1.6813106270345015	3.9531274573515054
11	44.6737230674275	23.121433594758443	17.336253223303334	14.868590114510727
12	38.47726551117363	14.508054920044271	23.562087721328552	23.45259184745355
13	27.6626933611079	14.231496235893875	27.893554580280654	30.212255822717577
14	25.47040198932341	15.735655001943627	31.479915370668753	27.31402763806421
15	27.514918441894487	18.754655058323618	28.3674432268345	25.3629832729474
16	36.41138401004157	18.54248825664171	22.77692218671272	22.269205546603995
17	34.96746281146235	20.04011881340894	21.981370864601974	23.011047510526737
18	31.88318066225716	22.76327229457654	22.28760322730928	23.065943815857022
19	32.45113486983641	23.13181938225336	20.333888231122348	24.083157516787885
20	33.09327327380793	23.6739574894881	21.17395007106846	22.05881916563551
21	33.71434336600405	22.647248211419026	22.63330158249728	21.005106840079645
22	31.600687242395374	20.12261163979715	25.859720651990063	22.416980465817407
23	31.095937970142344	19.82765527494147	25.715506574203484	23.3609001807127
24	33.65084169389227	18.430025014911024	23.406004172119204	24.513129119077504
25	29.8327294739747	22.246060077329606	20.354659806112185	27.566550642583508
26	27.528271597245098	21.549322104813367	23.403927014620223	27.51847928332132
27	24.391467036994175	19.665933726806312	25.27010465906427	30.672494577135247
28	26.556161887720737	22.911937424146657	21.787305006839784	28.74459568129282
29	31.01255493339743	23.48256226279603	20.912524962982086	24.59235784082445
30	30.076647111712496	20.268012664726008	23.81461072584785	25.840729497713642
31	32.2956447941982	20.071276175893697	20.41103979537031	27.22203923453779
32	35.71642645823875	20.69086258416197	21.488194326986136	22.104516630613148
33	37.09981335256188	18.946940495372388	21.96148949996884	21.991756652096893
34	29.382579770266382	21.77157795720462	27.681091041813183	21.164751230715815
35	28.87723702444221	25.45764230754394	26.16446933076953	19.500651337244324
36	27.666847676105867	28.235098620470684	22.51045255327167	21.58760115015178
37	31.428579906765304	27.2517129130947	18.68640559764272	22.63330158249728
38	31.474574108528515	28.721153475232864	18.833883780070565	20.97038863616806
39	33.04401496740346	24.244285591351904	18.78907652544963	23.922622915795003
40	30.100386054558026	22.27454680874424	19.110145727435395	28.51492140926234
41	25.827673079148607	24.78048896287526	21.959412342469857	27.43242561550628
42	24.227371594574464	21.660004925830638	20.932109590829644	33.18051388876525
43	24.203632651728938	20.873058970501397	24.511942171935228	30.41136620583444
44	27.516995599393468	20.476618624981082	23.96653996005923	28.039845815566217
45	30.501574188647446	19.332995053397784	21.80807658182962	28.35735417612515
46	26.887023403630277	23.74725147552367	21.373357190970893	27.99236792987516
47	25.846960970210592	20.758815308057294	23.775144733367163	29.61907898836495
48	28.69415042774608	21.276917735660934	25.548740500713652	24.480191335879333
49	30.270119495903547	19.701838877860173	23.865649452965737	26.162392173270543
50	25.714913100632348	20.26089098187235	25.45348799254597	28.570707924949335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	2.5
12	2.0
13	37.5
14	73.0
15	89.0
16	105.0
17	199.5
18	294.0
19	208.5
20	123.0
21	324.0
22	525.0
23	462.0
24	399.0
25	518.5
26	638.0
27	911.5
28	1185.0
29	1152.5
30	1120.0
31	1256.0
32	1392.0
33	1644.5
34	1897.0
35	2016.0
36	2135.0
37	2693.5
38	3252.0
39	4009.0
40	4766.0
41	4967.0
42	5168.0
43	5911.5
44	6655.0
45	7712.0
46	8769.0
47	9048.5
48	9328.0
49	9553.0
50	9778.0
51	10370.0
52	10962.0
53	13116.0
54	15270.0
55	16606.5
56	17943.0
57	18344.0
58	18745.0
59	24681.5
60	30618.0
61	32885.5
62	35153.0
63	36232.5
64	37312.0
65	39985.5
66	42659.0
67	33807.5
68	24956.0
69	20112.5
70	15269.0
71	12980.5
72	10692.0
73	9220.5
74	7749.0
75	6063.0
76	4377.0
77	4280.0
78	4183.0
79	3370.0
80	2557.0
81	1654.0
82	751.0
83	451.5
84	152.0
85	88.5
86	25.0
87	17.0
88	9.0
89	8.5
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	336999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.84245947317351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.99892613846103	6.012777485986605
2	8.98352293701131	1.5887287499369434
3	3.3994429343266552	0.9017830913444846
4	1.5873015873015872	0.5614259982967308
5	0.8658008658008658	0.38279045338413464
6	0.526863317560992	0.27952605200608904
7	0.3926306251887647	0.24302742738109015
8	0.2986677405282056	0.2112765913251968
9	0.3255142790026511	0.2590512138018214
>10	9.164737071713816	24.17633286745658
>50	4.560555723346421	27.803049860682076
>100	1.7886506258599282	25.879898753408764
>500	0.07047216349541931	3.7842842263626895
>1k	0.033558173093056816	5.835922361787424
>5k	0.0033558173093056814	2.0801248668393675
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7010	2.0801248668393675	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4111	1.2198849254745563	No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2751	0.8163228971005848	No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2745	0.8145424763871703	No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	2352	0.6979249196585153	No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1533	0.4548974922774252	No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1318	0.3910990833800694	No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1254	0.3721079291036472	No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1237	0.36706340374897256	No Hit
CCGCTAGGGGGGCTGGCAAGATGGCTCAGCAGGTAAGAGCACCGACTACT	1199	0.35578740589734686	No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1167	0.3462918287591358	No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	847	0.25133605737702486	No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	770	0.22848732488820442	No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	743	0.22047543167783878	No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	719	0.2133537488241805	No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	658	0.1952528049044656	No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	656	0.19465933133332738	No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	619	0.18368007026727082	No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	602	0.17863554491259617	No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	600	0.17804207134145797	No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	595	0.17655838741361252	No Hit
CCGCTAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC	576	0.17092038848779967	No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	565	0.1676562838465396	No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	556	0.16498565277641772	No Hit
CCGCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC	555	0.16468891599084864	No Hit
CCGCTAGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGG	545	0.16172154813515766	No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	536	0.1590509170650358	No Hit
CCGCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	535	0.15875418027946672	No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	530	0.15727049635162121	No Hit
CCGCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	524	0.15549007563820666	No Hit
CCGCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGC	519	0.15400639171036115	No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	503	0.1492586031412556	No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGC	483	0.14332386742987369	No Hit
CCGCTAGGGGCGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCG	475	0.1409499731453209	No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA	470	0.13946628921747542	No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	452	0.1341250270772317	No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	441	0.1308609224359716	No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	438	0.12997071207926433	No Hit
CCGCTAGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCT	431	0.12789355458028065	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	430	0.12759681779471155	No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	411	0.12195881886889871	No Hit
CCGCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	404	0.11988166136991504	No Hit
CCGCTAGGGGGAGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC	400	0.11869471422763866	No Hit
CCGCTAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCT	388	0.1151338728008095	No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTG	382	0.11335345208739493	No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	373	0.11068282101727306	No Hit
CCGCTAGGGAGGGCACCGCCAGTGTGTTTGCACCAGGAGACAGTAAAGGA	365	0.10830892673272027	No Hit
CCGCTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT	363	0.10771545316158208	No Hit
CCGCTAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGG	361	0.1071219795904439	No Hit
CCGCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	356	0.1056382956625984	No Hit
CCGCTAGGGGGGCCCCAGAAAGGACTCTTTCTCCCTGAGTTTTACAGTAA	352	0.10445134852032202	No Hit
CCGCTAGGGGTCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC	343	0.10178071745020015	No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGC	338	0.10029703352235465	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.018991154276422187	0.0	0.0	0.0	0.0
7	0.020771574989836766	0.0	0.0	0.0	0.0
8	0.021661785346544057	0.0	0.0	0.0	0.0
9	0.028783468200202375	0.0	0.0	0.0	0.0
10	0.04273009712194992	0.0	0.0	0.0	0.0
11	0.06795272389532313	0.0	0.0	0.0	0.0
12	0.07329398603556687	0.0	0.0	0.0	0.0
13	0.07685482746239602	0.0	0.0	0.0	0.0
14	0.0887242988851599	0.0	0.0	0.0	0.0
15	0.11602408315751679	0.0	0.0	0.0	0.0
16	0.13026744886483343	0.0	0.0	0.0	0.0
17	0.13857607886076814	0.0	0.0	0.0	0.0
18	0.14777491921341013	0.0	0.0	0.0	0.0
19	0.16617259991869412	0.0	0.0	0.0	0.0
20	0.17893228169816527	0.0	0.0	0.0	0.0
21	0.1857572277662545	0.0	0.0	0.0	0.0
22	0.19703322561788017	0.0	0.0	0.0	0.0
23	0.20623206597052215	0.0	0.0	0.0	0.0
24	0.21839827417885513	0.0	0.0	0.0	0.0
25	0.2293775352449117	0.0	0.0	0.0	0.0
26	0.2385763755975537	0.0	0.0	0.0	0.0
27	0.24896216309247207	0.0	0.0	0.0	0.0
28	0.2649859495132033	0.0	0.0	0.0	0.0
29	0.27863584164938177	0.0	0.0	0.0	0.0
30	0.28753794521645465	0.0	0.0	0.0	0.0
31	0.30296825806604766	0.0	0.0	0.0	0.0
32	0.3181018341300716	0.0	0.0	0.0	0.0
33	0.334719094121941	0.0	0.0	0.0	0.0
34	0.3480722494725504	0.0	0.0	0.0	0.0
35	0.3629090887510052	0.0	0.0	0.0	0.0
36	0.3798230855284437	0.0	0.0	0.0	0.0
37	0.3964403455203131	0.0	0.0	0.0	0.0
38	0.40949676408535335	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGCC	130	0.0	44.000004	5
ATTTATA	20	7.848553E-4	44.0	25
GTGCATA	30	2.5223726E-6	44.0	33
TTGATAC	30	2.5223726E-6	44.0	16
ATCCTAG	30	2.5223726E-6	44.0	10
AGACTTA	30	2.5223726E-6	44.0	44
CGGAATT	20	7.848553E-4	44.0	25
CGGAAAT	30	2.5223726E-6	44.0	36
TCGTTCT	20	7.848553E-4	44.0	23
TCGTTCC	20	7.848553E-4	44.0	27
GATACCA	30	2.5223726E-6	44.0	9
CTCACGC	25	4.4361208E-5	44.0	25
ACCGGAT	30	2.5223726E-6	44.0	30
ATTAGTC	20	7.848553E-4	44.0	18
GCGACGT	20	7.848553E-4	44.0	17
CCCTCGA	20	7.848553E-4	44.0	21
TCCGCGC	25	4.4361208E-5	44.0	13
ACGCCCA	25	4.4361208E-5	44.0	36
GATCGTA	20	7.848553E-4	44.0	36
CTCGTAA	25	4.4361208E-5	44.0	43
>>END_MODULE