FastQCFastQC Report
Fri 17 Jun 2016
SRR1527776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507040
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC103512.0414562953613125No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT56931.1227911012937835No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG43030.8486509940044178No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41330.8151230672136321No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC34620.682786367939413No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC22850.45065478068791415No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT20330.40095455979804356No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG19230.3792600189334175No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18790.3705822025875671No Hit
CCGCTAGGGGGGCTGGCAAGATGGCTCAGCAGGTAAGAGCACCGACTACTCT18540.3656516251183339No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG18150.35795992426633005No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA12330.24317608078258127No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT11740.2315399179551909No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10810.21319816976964343No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC10590.2088592615967182No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT10280.20274534553486903No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT10200.20116756074471442No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC10060.1984064373619438No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC8980.17710634269485642No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA8840.17434521931208583No Hit
CCGCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT8630.17020353423792994No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT8590.16941464184285263No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT8410.16586462606500474No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG8390.1654701798674661No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT8200.16172294099084883No Hit
CCGCTAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG8000.1577784790154623No Hit
CCGCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG7980.15738403281792362No Hit
CCGCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG7890.1556090249289997No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG7890.1556090249289997No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT7730.15245345534869045No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC7440.14673398548437994No Hit
CCGCTAGGGGCGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGG7410.14614231618807194No Hit
CCGCTAGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAA7300.14397286210160934No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC6970.13746449984222153No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC6950.13707005364468286No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT6740.13292836857052698No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC6630.13075891448406438No Hit
CCGCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG6520.12858946039760177No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC6510.12839223729883242No Hit
CCGCTAGGGGGAGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC6370.12563111391606185No Hit
CCGCTAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG6370.12563111391606185No Hit
CCGCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC6250.12326443673082992No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA6190.12208109813821395No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA5870.11576995897759546No Hit
CCGCTAGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTC5840.11517828968128747No Hit
CCGCTAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG5710.11261438939728621No Hit
CCGCTAGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT5580.11005048911328495No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT5510.10866992742189965No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT5470.10788103502682234No Hit
CCGCTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG5340.10531713474282109No Hit
CCGCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5330.10511991164405174No Hit
CCGCTAGGGAGGGCACCGCCAGTGTGTTTGCACCAGGAGACAGTAAAGGAGC5290.10433101924897444No Hit
CCGCTAGGGGGGCCCCAGAAAGGACTCTTTCTCCCTGAGTTTTACAGTAATG5260.10373934995266645No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTTAGAGGTTGAAAGCACTGACTGCTC5180.10216156516251182No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACTGCTC5130.10117544966866521No Hit
CCGCTAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC5120.10097822656989586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTA351.018052E-746.00000420
CTCACGC351.018052E-746.00000425
CGTATAG351.018052E-746.00000429
TCTTACC351.018052E-746.00000423
TACGGTT351.018052E-746.00000411
CCGTATA351.018052E-746.00000428
ACTAGGT351.018052E-746.00000444
AACGAAA351.018052E-746.00000426
TAGGGCC1700.046.0000045
CAAGCGA351.018052E-746.00000433
ACGTCCC351.018052E-746.00000411
TATAGGT351.018052E-746.00000431
ACCGTAT351.018052E-746.00000427
CACGTCT850.046.00000440
CCACGTA351.018052E-746.00000423
CTGATCA351.018052E-746.00000418
CTAGGTC351.018052E-746.00000445
GCCCGCC850.046.00000410
GCATAAT301.859331E-646.040
TTGATTC206.3081004E-446.014
Produced by FastQC (version 0.11.5)