FastQCFastQC Report
Fri 17 Jun 2016
SRR1527775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511516
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC95981.8763831434402836No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT55561.0861830323978134No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG40800.7976290086722605No Hit
CCGCTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC39000.7624394935837785No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC34190.6684052893751123No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC21890.4279436029371515No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT19630.38376121177050176No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18510.36186551349322404No Hit
CCGCTAGGGGGGCTGGCAAGATGGCTCAGCAGGTAAGAGCACCGACTACTCT18140.3546321131694805No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG17900.34994017782434955No Hit
CCGCTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT17500.34212028558246466No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT11540.22560389117837956No Hit
CCGCTAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA11320.22130295044534287No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA10020.19588830065921692No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT9450.18474495421453094No Hit
CCGCTAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT9320.18220348923591834No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC9310.18200799192987122No Hit
CCGCTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9160.17907553233916437No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT8590.16793218589447836No Hit
CCGCTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG8570.16754119128238415No Hit
CCGCTAGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC8540.16695469936424276No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT8520.16656370475214852No Hit
CCGCTAGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG8440.16499972630377155No Hit
CCGCTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT8300.16226276401911183No Hit
CCGCTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA8030.1569843367558395No Hit
CCGCTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT7720.1509239202683787No Hit
CCGCTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG7590.14838245528976612No Hit
CCGCTAGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG7190.1405625630478812No Hit
CCGCTAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT7140.1395850765176456No Hit
CCGCTAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC7110.1389985845995042No Hit
CCGCTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG6970.1362616223148445No Hit
CCGCTAGGGGCGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGG6950.13587062770275027No Hit
CCGCTAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC6870.13430664925437327No Hit
CCGCTAGGGATCCGATTAGCCCAACTCTGTCTCCCAGGCCCCCTGCTGGGAA6680.13059220043947795No Hit
CCGCTAGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC6640.12981021121528946No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC6630.1296147139092423No Hit
CCGCTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG6550.12805073546086537No Hit
CCGCTAGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT6460.12629125970644123No Hit
CCGCTAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC6080.11886236207665059No Hit
CCGCTAGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC6010.11749388093432071No Hit
CCGCTAGGGGGAGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC5840.11417042673151964No Hit
CCGCTAGGGGAGAGCGCGGGACGTCTGTCTTCGAGTCCGAACGTTCGTGGTG5790.11319294020128402No Hit
CCGCTAGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTC5790.11319294020128402No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA5770.11280194558918978No Hit
CCGCTAGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGG5700.11143346444685992No Hit
CCGCTAGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT5580.10908749677429445No Hit
CCGCTAGGGGGGCCCCAGAAAGGACTCTTTCTCCCTGAGTTTTACAGTAATG5480.10713252371382322No Hit
CCGCTAGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT5360.10478655604125775No Hit
CCGCTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5310.10380906951102213No Hit
CCGCTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA5210.10185409645055092No Hit
CCGCTAGGGAGGGCACCGCCAGTGTGTTTGCACCAGGAGACAGTAAAGGAGC5200.10165859914450379No Hit
CCGCTAGGGGAGTAGCAGCAGAGACAGCGGCTGCGGCAGCACTCCAGTCCAT5130.10029011800217394No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT5120.10009462069612682No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAT206.308152E-446.00000416
TCACGAC206.308152E-446.00000441
CTATGTG206.308152E-446.00000433
ACTATTA206.308152E-446.00000422
TAATAAC206.308152E-446.00000417
ACAGTCG206.308152E-446.00000437
CGAACTT206.308152E-446.00000418
TCGCAAC206.308152E-446.00000415
CTCGTAA206.308152E-446.00000443
CGTATAT206.308152E-446.00000417
TACTTAT206.308152E-446.00000430
TTTCGCA206.308152E-446.00000413
CGCATGG206.308152E-446.00000426
CGGTTAT405.6006684E-946.00000437
CGTTGAC206.308152E-446.00000431
ATTACGA206.308152E-446.00000411
ACACACG206.308152E-446.00000434
ACACAAT206.308152E-446.00000419
CCTACGC206.308152E-446.00000433
CAATTAC206.308152E-446.00000441
Produced by FastQC (version 0.11.5)