FastQCFastQC Report
Fri 17 Jun 2016
SRR1527774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076431
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC38800.3604504143786272No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC38500.3576634266385862No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC33370.3100059362838863No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT29200.27126680669731734No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC28900.2684798189572764No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19360.17985360882397478No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGA17240.16015889546101886No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG17220.1599730962783495No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG16860.15662871099030035No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG15250.141671876785414No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG14560.13526180498331988No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT14200.13191741969527077No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC14070.1307097250079197No Hit
TACTTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC13390.12439255279716026No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA13350.12402095443182146No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAA12010.11157240919297197No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT10850.10079605659814703No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10770.10005285986746944No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG254.4426524E-544.032
GTCGATA207.8562583E-444.028
ATAATAC207.8562583E-444.014
GCACGAC351.4459692E-744.024
ATTCGTC408.310963E-944.024
CGATTGT207.8562583E-444.043
GGATACG700.044.08
TCGATCT207.8562583E-444.028
ATCACGA207.8562583E-444.030
ATTCACG207.8562583E-444.044
CTCGGTA207.8562583E-444.026
TACGATC408.310963E-944.011
CTATTCG302.5275658E-644.039
CGAGATA302.5275658E-644.019
TATACCC254.4426524E-544.023
CAATCGT207.8562583E-444.023
TATCGCG207.8562583E-444.027
CGATAGG207.8562583E-444.030
AATCGCA254.4426524E-544.031
ACGAACA454.802132E-1044.038
Produced by FastQC (version 0.11.5)