##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1076431
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67017579389668	34.0	31.0	34.0	31.0	34.0
2	32.99944446044382	34.0	31.0	34.0	31.0	34.0
3	33.17125575164595	34.0	34.0	34.0	31.0	34.0
4	36.514944292760056	37.0	37.0	37.0	35.0	37.0
5	36.56615333449148	37.0	37.0	37.0	35.0	37.0
6	36.40079391990755	37.0	37.0	37.0	35.0	37.0
7	36.54386579353437	37.0	37.0	37.0	35.0	37.0
8	36.6753345082035	37.0	37.0	37.0	35.0	37.0
9	38.678976172183816	39.0	39.0	39.0	39.0	39.0
10	38.179692892530966	39.0	39.0	39.0	37.0	39.0
11	38.00414517976535	39.0	38.0	39.0	37.0	39.0
12	37.772077355631716	39.0	38.0	39.0	35.0	39.0
13	37.68618610946731	39.0	38.0	39.0	35.0	39.0
14	38.54738297206231	40.0	39.0	40.0	35.0	40.0
15	38.56209733833381	40.0	38.0	40.0	35.0	40.0
16	38.570430431676535	40.0	38.0	40.0	35.0	40.0
17	38.53837821467423	40.0	38.0	40.0	35.0	40.0
18	38.44063112266369	40.0	38.0	40.0	35.0	40.0
19	38.41295261842143	40.0	38.0	40.0	35.0	40.0
20	38.31219557965164	40.0	38.0	40.0	35.0	40.0
21	38.31390400313629	40.0	38.0	40.0	35.0	40.0
22	38.32135176337359	40.0	38.0	40.0	35.0	40.0
23	38.17743264547379	40.0	38.0	40.0	35.0	40.0
24	38.067907743273835	40.0	38.0	40.0	34.0	40.0
25	37.9589476705892	40.0	38.0	40.0	34.0	40.0
26	37.81971069209266	40.0	37.0	40.0	34.0	40.0
27	37.687557307435405	40.0	37.0	40.0	34.0	40.0
28	37.52319377647058	40.0	37.0	40.0	33.0	40.0
29	37.49815269162631	40.0	37.0	40.0	33.0	40.0
30	37.59630947083464	40.0	37.0	40.0	33.0	40.0
31	37.58464685613848	40.0	37.0	40.0	33.0	40.0
32	37.515445950553264	40.0	37.0	40.0	33.0	40.0
33	37.3595372113958	40.0	36.0	40.0	33.0	40.0
34	37.07191914762767	40.0	36.0	40.0	33.0	40.0
35	36.99761062251087	40.0	36.0	40.0	33.0	40.0
36	36.81932051380906	40.0	35.0	40.0	32.0	40.0
37	36.62433913553214	40.0	35.0	40.0	31.0	40.0
38	36.46284434394773	40.0	35.0	40.0	31.0	40.0
39	36.25820047917609	39.0	35.0	40.0	31.0	40.0
40	36.03648352750896	39.0	35.0	40.0	30.0	40.0
41	35.86057257734123	39.0	35.0	40.0	30.0	40.0
42	35.71314278388489	39.0	35.0	40.0	29.0	40.0
43	35.54738297206231	38.0	35.0	40.0	29.0	40.0
44	35.22279551592253	38.0	35.0	40.0	27.0	40.0
45	35.18679135030485	38.0	35.0	40.0	28.0	40.0
46	34.95938708565621	37.0	35.0	40.0	26.0	40.0
47	34.85638559275978	37.0	35.0	40.0	26.0	40.0
48	34.680800720157634	37.0	34.0	40.0	26.0	40.0
49	34.55033996605449	36.0	34.0	40.0	26.0	40.0
50	34.05562548830348	35.0	34.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	5.0
14	2.0
15	17.0
16	32.0
17	87.0
18	184.0
19	387.0
20	687.0
21	1226.0
22	1838.0
23	2954.0
24	4328.0
25	5816.0
26	7877.0
27	10228.0
28	13264.0
29	15642.0
30	16492.0
31	17930.0
32	21544.0
33	27524.0
34	46409.0
35	67789.0
36	93783.0
37	173122.0
38	327146.0
39	220116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.41324618113005	0.3650024943540273	0.14390146697744677	0.07784985753847669
8	98.41569036937805	1.2080662857164093	0.11203690714964545	0.2642064377558803
9	93.81140082364777	3.5006424006740797	1.288610231403592	1.3993465442745516
10	45.175491973010814	38.58723875473672	6.680409612878114	9.556859659374359
11	31.036267071461154	26.042542438855808	23.581167766442995	19.34002272324004
12	27.937601202492313	20.69979404160601	27.13039665338512	24.232208102516555
13	23.26252216816498	20.513809059753946	30.69291018188811	25.53075859019296
14	24.762293170672343	23.405959137185757	27.19765595751144	24.63409173463046
15	25.14234539882259	22.650685459634662	26.572441707828926	25.63452743371382
16	28.280029096152003	23.30023940224687	24.319533718371172	24.10019778322995
17	28.541634345350513	22.991255361467665	23.55376238699926	24.913347906182562
18	27.997985936859866	23.29866010919418	23.111746131428767	25.591607822517187
19	28.547765718378603	23.0890786311431	23.033710474707622	25.329445175770672
20	28.071005015648936	24.074929094386913	23.53601856505433	24.318047324909816
21	28.563001251357495	23.063438343934724	25.374594377159337	22.998966027548445
22	28.693432277591413	22.39549028223825	22.633406135646407	26.27767130452393
23	26.309814563125737	25.255775799842255	23.752753311638184	24.681656325393824
24	26.91765658922866	23.30516308058761	23.927961940895422	25.849218389288303
25	25.406644736169802	24.030244390954923	23.687166200155886	26.87594467271939
26	25.87457997772268	23.949143047719733	23.29039204556539	26.885884928992198
27	27.475518635193524	21.422088364233286	23.38830821483216	27.71408478574103
28	29.03911165694782	24.353163370434334	22.054177183674568	24.553547788943277
29	28.466292776778072	22.81874082035913	23.542428636856428	25.17253776600637
30	27.781901487415357	23.744578147600727	22.994692646347048	25.478827718636865
31	29.53826116118915	24.723182442720436	21.40276524923567	24.335791146854746
32	30.351132585367758	24.081339166189007	22.09830448955855	23.469223758884684
33	29.234386597933355	24.694011971041338	22.666106791796224	23.405494639229083
34	27.263521767767745	26.74551364648547	23.535832765871664	22.455131819875124
35	28.295264629130894	25.933199619854875	23.972925343101416	21.798610407912815
36	28.324342201218656	26.96122649756464	21.484145291244864	23.23028600997184
37	28.203479832892214	26.122993484951664	22.03996354620036	23.633563135955765
38	29.63487673617724	25.86993499815594	21.396355177433573	23.098833088233246
39	28.523983422996924	25.447148957991732	21.275957307063806	24.752910311947538
40	27.907037236943193	26.16749238920098	21.780959485559222	24.1445108882966
41	26.12159999108164	25.950200245069123	22.7463720387094	25.18182772513984
42	26.403271552008444	24.491490861931698	23.585348248053055	25.519889338006802
43	26.275441714331897	23.841565320954153	24.302161494791584	25.580831469922366
44	25.77963659537862	24.49446364885441	24.406301936677778	25.319597819089196
45	26.539090754539767	24.641709501119905	23.545494323370473	25.273705420969854
46	26.210969397945615	25.0138652640067	22.833511855381346	25.941653482666325
47	26.32439979896529	25.880339752385428	23.35021938238494	24.445041066264352
48	26.815838637125832	24.95803260961455	23.79678771793083	24.429341035328786
49	25.32860907944866	25.54060594687444	23.634492131869113	25.496292841807787
50	23.897769573711646	26.17055807571502	23.05990815946401	26.87176419110932
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	19.0
4	38.0
5	255.5
6	473.0
7	378.5
8	284.0
9	1635.0
10	2986.0
11	3028.5
12	3071.0
13	3410.0
14	3749.0
15	3270.5
16	2792.0
17	3286.5
18	3781.0
19	3701.5
20	3622.0
21	4136.0
22	4650.0
23	5787.5
24	6925.0
25	8653.0
26	10381.0
27	10661.0
28	10941.0
29	12611.5
30	14282.0
31	16341.5
32	18401.0
33	19881.5
34	21362.0
35	20957.0
36	20552.0
37	23219.0
38	25886.0
39	25908.0
40	25930.0
41	27319.5
42	28709.0
43	33388.5
44	38068.0
45	39958.5
46	41849.0
47	44899.5
48	47950.0
49	52458.5
50	56967.0
51	61738.0
52	66509.0
53	71883.0
54	77257.0
55	85206.0
56	93155.0
57	92788.0
58	92421.0
59	88293.0
60	84165.0
61	81886.5
62	79608.0
63	69374.5
64	59141.0
65	51680.0
66	44219.0
67	35706.0
68	27193.0
69	23858.5
70	20524.0
71	17096.0
72	13668.0
73	14496.5
74	15325.0
75	10654.5
76	5984.0
77	3891.5
78	1799.0
79	1535.5
80	1272.0
81	831.0
82	390.0
83	254.0
84	118.0
85	68.5
86	19.0
87	16.0
88	13.0
89	7.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1076431.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.599805219112424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37626213053758	9.707032622436214
2	9.03721154307506	2.458086334194705
3	3.58717736096768	1.4635474018671055
4	1.8081480403360477	0.9836184462356031
5	1.124993990218064	0.7649849569818868
6	0.7277192837015101	0.5938104307519531
7	0.5598137889381339	0.5329350941972354
8	0.4634735083191119	0.5042519549886884
9	0.3453756726536219	0.42273376879582725
>10	7.109398936829317	27.13901010353928
>50	2.8086538596733246	25.8769395078432
>100	1.0046258806878912	22.928847805337625
>500	0.03327953227951606	3.050114194097435
>1k	0.013866471783131691	3.574087378733253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3880	0.3604504143786272	No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3850	0.3576634266385862	No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3337	0.3100059362838863	No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2920	0.27126680669731734	No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2890	0.2684798189572764	No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1936	0.17985360882397478	No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGA	1724	0.16015889546101886	No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1722	0.1599730962783495	No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1686	0.15662871099030035	No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1525	0.141671876785414	No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1456	0.13526180498331988	No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1420	0.13191741969527077	No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1407	0.1307097250079197	No Hit
TACTTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	1339	0.12439255279716026	No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1335	0.12402095443182146	No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAA	1201	0.11157240919297197	No Hit
TACTTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1085	0.10079605659814703	No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1077	0.10005285986746944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001950891418028652	0.0	0.0	0.0	0.0
8	0.013563340334865868	0.0	0.0	0.0	0.0
9	0.02118110682431108	0.0	0.0	0.0	0.0
10	0.08676821830660766	0.0	0.0	0.0	0.0
11	0.13628370048800156	0.0	0.0	0.0	0.0
12	0.17028495091650092	0.0	0.0	0.0	0.0
13	0.20837378336372697	0.0	0.0	0.0	0.0
14	0.24674131458495713	0.0	0.0	0.0	0.0
15	0.30489645876047794	0.0	0.0	0.0	0.0
16	0.36035751478729244	0.0	0.0	0.0	0.0
17	0.4069931096373107	0.0	0.0	0.0	0.0
18	0.44870502614658997	0.0	0.0	0.0	0.0
19	0.4952477214052735	0.0	0.0	0.0	0.0
20	0.5430910109426429	0.0	0.0	0.0	0.0
21	0.5887976098793142	0.0	0.0	0.0	0.0
22	0.6355261043206671	0.0	0.0	0.0	0.0
23	0.6894078672947918	0.0	0.0	0.0	0.0
24	0.7481204090183208	0.0	0.0	0.0	0.0
25	0.799958380983082	0.0	0.0	0.0	0.0
26	0.8595070190286233	0.0	0.0	0.0	0.0
27	0.9169189664734665	0.0	0.0	0.0	0.0
28	0.984364069782457	0.0	0.0	0.0	0.0
29	1.0265404842484098	0.0	0.0	0.0	0.0
30	1.0708535893150606	0.0	0.0	0.0	0.0
31	1.1055980364742375	0.0	0.0	0.0	0.0
32	1.1418288770947698	0.0	0.0	0.0	0.0
33	1.1725786418265547	0.0	0.0	0.0	0.0
34	1.212711265283144	0.0	0.0	0.0	0.0
35	1.2591610609504928	0.0	0.0	0.0	0.0
36	1.290189524456282	0.0	0.0	0.0	0.0
37	1.3232617789714343	0.0	0.0	0.0	0.0
38	1.3551263387992356	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	25	4.4426524E-5	44.0	32
GTCGATA	20	7.8562583E-4	44.0	28
ATAATAC	20	7.8562583E-4	44.0	14
GCACGAC	35	1.4459692E-7	44.0	24
ATTCGTC	40	8.310963E-9	44.0	24
CGATTGT	20	7.8562583E-4	44.0	43
GGATACG	70	0.0	44.0	8
TCGATCT	20	7.8562583E-4	44.0	28
ATCACGA	20	7.8562583E-4	44.0	30
ATTCACG	20	7.8562583E-4	44.0	44
CTCGGTA	20	7.8562583E-4	44.0	26
TACGATC	40	8.310963E-9	44.0	11
CTATTCG	30	2.5275658E-6	44.0	39
CGAGATA	30	2.5275658E-6	44.0	19
TATACCC	25	4.4426524E-5	44.0	23
CAATCGT	20	7.8562583E-4	44.0	23
TATCGCG	20	7.8562583E-4	44.0	27
CGATAGG	20	7.8562583E-4	44.0	30
AATCGCA	25	4.4426524E-5	44.0	31
ACGAACA	45	4.802132E-10	44.0	38
>>END_MODULE