FastQCFastQC Report
Fri 17 Jun 2016
SRR1527773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619859
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC60720.3748474404253704No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC57760.35657424504231544No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT48530.2995939770066407No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG45080.2782958269824719No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC41650.25712114449467516No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26570.16402662207019253No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC25730.1588409855425688No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT23650.14600036175988157No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT23260.1435927448006277No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT22140.1366785627637961No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT21180.13075212101794045No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC20380.12581341956306075No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG20290.12525781564938676No Hit
TACTTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC20220.12482567927208478No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT19670.121430322021855No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA16480.10173724997052214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC206.3122244E-446.029
CTCGTTA301.8618775E-646.012
AATTTCG253.4177803E-546.014
GTCGATA206.3122244E-446.028
CGAAATA206.3122244E-446.015
TTTCGAA206.3122244E-446.039
CGCATAC206.3122244E-446.044
TCGATAG206.3122244E-446.029
CCGTATA206.3122244E-446.018
TTGCGAT206.3122244E-446.032
TCGTACC600.046.038
CTATTCG405.6134013E-946.039
CGTAACA501.6370905E-1146.035
ATCGGTT206.3122244E-446.021
GACGAAT206.3122244E-446.043
CGATCTA301.8618775E-646.041
TCGACAC206.3122244E-446.042
GTTATAT301.8618775E-646.013
ACGAATA253.4177803E-546.027
GCATATA253.4177803E-546.037
Produced by FastQC (version 0.11.5)