##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527773_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1619859 Sequences flagged as poor quality 0 Sequence length 52 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30667360554221 33.0 31.0 34.0 31.0 34.0 2 32.67767626688496 34.0 31.0 34.0 31.0 34.0 3 32.877965921725284 34.0 31.0 34.0 31.0 34.0 4 36.36528858375945 37.0 37.0 37.0 35.0 37.0 5 36.378377994627925 37.0 37.0 37.0 35.0 37.0 6 36.17305765501812 37.0 37.0 37.0 35.0 37.0 7 36.43992717884705 37.0 37.0 37.0 35.0 37.0 8 36.5918873185876 37.0 37.0 37.0 35.0 37.0 9 38.60270245743611 39.0 39.0 39.0 37.0 39.0 10 37.92737701244368 39.0 38.0 39.0 35.0 39.0 11 37.64648404583362 39.0 38.0 39.0 35.0 39.0 12 37.408196021999444 39.0 37.0 39.0 35.0 39.0 13 37.32257066818779 39.0 37.0 39.0 34.0 39.0 14 38.19608743723991 40.0 38.0 40.0 34.0 40.0 15 38.23170164810641 40.0 38.0 40.0 34.0 40.0 16 38.2375589480319 40.0 38.0 40.0 35.0 40.0 17 38.19165618735952 40.0 38.0 40.0 34.0 40.0 18 38.156685859695195 40.0 38.0 40.0 34.0 40.0 19 38.13218496177754 40.0 38.0 40.0 34.0 40.0 20 38.08904108320539 40.0 38.0 40.0 34.0 40.0 21 38.0706431856106 40.0 38.0 40.0 34.0 40.0 22 38.05962802935317 40.0 38.0 40.0 34.0 40.0 23 37.94587306672988 40.0 38.0 40.0 34.0 40.0 24 37.831668064936515 40.0 37.0 40.0 34.0 40.0 25 37.728310920888795 40.0 37.0 40.0 34.0 40.0 26 37.563976864653036 40.0 37.0 40.0 33.0 40.0 27 37.453444404729055 40.0 36.0 40.0 33.0 40.0 28 37.30774406908255 40.0 36.0 40.0 33.0 40.0 29 37.18808365419459 40.0 36.0 40.0 33.0 40.0 30 37.0558869630011 40.0 36.0 40.0 32.0 40.0 31 36.94461801922266 40.0 35.0 40.0 32.0 40.0 32 36.76597345818371 39.0 35.0 40.0 31.0 40.0 33 36.583258172470565 39.0 35.0 40.0 31.0 40.0 34 36.48548237840454 39.0 35.0 40.0 31.0 40.0 35 36.30047121385256 39.0 35.0 40.0 30.0 40.0 36 36.14422983728831 39.0 35.0 40.0 30.0 40.0 37 35.910128597612506 39.0 35.0 40.0 30.0 40.0 38 35.76500485536087 38.0 35.0 40.0 29.0 40.0 39 35.54838600149766 38.0 35.0 40.0 28.0 40.0 40 35.320927315278674 38.0 35.0 40.0 28.0 40.0 41 35.145873807535104 38.0 34.0 40.0 27.0 40.0 42 34.993200642771995 38.0 34.0 40.0 27.0 40.0 43 34.79350671879466 37.0 34.0 40.0 26.0 40.0 44 34.62854853416254 37.0 34.0 40.0 26.0 40.0 45 34.5282039980023 37.0 34.0 40.0 26.0 40.0 46 34.325699335559456 36.0 34.0 40.0 24.0 40.0 47 34.13750641259517 36.0 33.0 40.0 24.0 40.0 48 33.86746192106844 35.0 33.0 39.0 23.0 40.0 49 34.08650073864454 35.0 33.0 40.0 24.0 40.0 50 34.09336244697841 35.0 34.0 40.0 24.0 40.0 51 34.063871608578275 35.0 34.0 40.0 24.0 40.0 52 33.52511854426836 35.0 33.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 2.0 14 4.0 15 14.0 16 49.0 17 144.0 18 344.0 19 849.0 20 1487.0 21 2505.0 22 4093.0 23 6169.0 24 8788.0 25 11913.0 26 16255.0 27 21099.0 28 24542.0 29 26947.0 30 28943.0 31 33838.0 32 42538.0 33 55470.0 34 89393.0 35 118658.0 36 172886.0 37 278728.0 38 460636.0 39 213562.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.49884526986608 0.34039999777758434 0.10686115272995982 0.053893579626374886 8 98.49881995902112 1.162014718565011 0.0883410222741609 0.2508243001397035 9 93.76106191958684 3.517651845006263 1.2955448591513212 1.4257413762555875 10 45.229183527702105 38.52261215328001 6.676568763083701 9.57163555593419 11 31.070914196852932 25.892994390252483 23.63983531899999 19.396256093894593 12 27.947555929250633 20.520860148938887 27.14100424790059 24.39057967390989 13 23.4334593319542 20.2175004120729 30.747305784021943 25.601734471950955 14 24.81876509004796 23.31042393195951 27.229715672783865 24.641095305208662 15 25.276953117524425 22.40417221498908 26.62071204962901 25.698162617857477 16 28.375432676547774 22.948787517925943 24.453733318764165 24.22204648676212 17 28.64119654858849 22.731546387679423 23.535937387142955 25.091319676589137 18 28.17776115081621 23.018793611048864 23.031078630917875 25.772366607217045 19 28.589216715775876 22.838222339104824 23.14077953698439 25.431781408134906 20 28.083678888100756 23.786329550905357 23.708545002991002 24.421446558002888 21 28.78120873483433 22.656725060637996 25.409001647674273 23.153064556853405 22 28.833867639096983 22.16507733080472 22.615795572330676 26.385259457767617 23 26.410323367651134 24.966432263548867 23.796886025265163 24.82635834353484 24 26.929072221718066 23.101763795490843 23.978877173877482 25.990286808913616 25 25.50555326111717 23.75854935522166 23.643107208713847 27.092790174947325 26 25.942443138569466 23.758919757830775 23.358452803608216 26.940184299991543 27 27.599871346827097 21.227958729741292 23.383763648564475 27.788406274867132 28 29.122596472902888 24.07061355340187 22.163410519063696 24.643379454631546 29 28.457229919394216 22.58110119461015 23.645514825673096 25.316154060322532 30 28.014351866427877 23.41586520802119 23.029350085408666 25.54043284014226 31 29.69727612094633 24.456387870796163 21.384392098324607 24.461943909932902 32 30.41740052683598 23.772871589440808 22.28854486717671 23.5211830165465 33 29.420523638168504 24.336192224138024 22.714507867660085 23.52877627003338 34 27.172118067066332 26.46829137597779 23.649095384227888 22.710495172727995 35 28.355924805800996 25.659579012741236 24.00968232420229 21.974813857255477 36 28.431116535451544 26.651146797344705 21.546937109958336 23.370799557245412 37 28.35302331869626 25.85496638904991 22.063154879529637 23.728855412724194 38 29.750120226513545 25.613957758051782 21.47007856856677 23.165843446867907 39 28.62773858712394 25.108296462840286 21.268826484280424 24.995138465755353 40 27.99860975554045 25.940776326828445 21.846160684355862 24.214453233275243 41 26.167647924911986 25.648096531858638 22.947984978939527 25.236270564289853 42 26.564534320579753 24.246739994036517 23.651749936259883 25.53697574912384 43 26.382851840808367 23.62267333144428 24.36854071866749 25.62593410907986 44 25.719522501649834 24.223775032271327 24.570780543244812 25.485921922834027 45 26.68917479854728 24.284521060166348 23.606128681570432 25.42017545971594 46 26.11184059847184 24.833828129485344 22.875200866248235 26.179130405794577 47 26.481749337442334 25.61414295935634 23.334253166479304 24.569854536722026 48 27.022907549360774 24.626279200844024 23.775279206400064 24.575534043395137 49 25.329550288018893 25.274236831724245 23.817011233693798 25.579201646563064 50 23.860595274033113 26.059984233195603 23.135532166688584 26.943888326082703 51 24.82370379150284 25.785577633608852 23.659590124819506 25.731128450068802 52 26.176846256371693 25.220281518329678 23.839976195458988 24.762896029839634 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 22.5 4 45.0 5 357.0 6 669.0 7 506.5 8 344.0 9 2202.0 10 4060.0 11 4087.0 12 4114.0 13 4572.5 14 4301.5 15 3572.0 16 4266.0 17 4960.0 18 4674.0 19 4388.0 20 4869.0 21 5350.0 22 6522.5 23 7695.0 24 8967.5 25 10240.0 26 12825.0 27 15410.0 28 16376.0 29 17342.0 30 20364.5 31 23387.0 32 26549.5 33 29712.0 34 29241.5 35 28771.0 36 30037.5 37 31304.0 38 34713.0 39 38704.0 40 39286.0 41 42984.0 42 46682.0 43 50316.5 44 53951.0 45 56864.5 46 59778.0 47 65759.0 48 71740.0 49 75460.5 50 79181.0 51 88251.0 52 97321.0 53 103929.5 54 110538.0 55 119263.0 56 127988.0 57 134959.5 58 141931.0 59 134091.0 60 126251.0 61 122779.5 62 119308.0 63 105827.5 64 81036.0 65 69725.0 66 58436.0 67 47147.0 68 40349.0 69 33551.0 70 28865.5 71 24180.0 72 25222.5 73 26265.0 74 18562.0 75 10859.0 76 7501.5 77 4144.0 78 3042.0 79 1940.0 80 1449.0 81 958.0 82 562.5 83 167.0 84 123.0 85 79.0 86 49.0 87 19.0 88 12.5 89 3.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1619859.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.181329913752698 #Duplication Level Percentage of deduplicated Percentage of total 1 74.57573190488623 9.830073257978784 2 8.552689220730167 2.2547163653648163 3 3.5944005576256646 1.4213693877672167 4 1.8654083354533193 0.9835425077389786 5 1.1302632598321423 0.7449186458620529 6 0.7520465648803104 0.5947784329315077 7 0.4898613465735457 0.4519916814826739 8 0.39177433442517334 0.4131285403039273 9 0.34742654511557175 0.4121589520767274 >10 3.9280868482022697 13.702289358147123 >50 2.782124519348854 26.326250632670778 >100 1.5288815482877818 34.223675517052634 >500 0.04211794898864413 3.6879981689846324 >1k 0.018251111228412456 4.2222573246802835 >5k 9.359544219698694E-4 0.7308512269578658 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC 6072 0.3748474404253704 No Hit TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 5776 0.35657424504231544 No Hit TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT 4853 0.2995939770066407 No Hit TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG 4508 0.2782958269824719 No Hit TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC 4165 0.25712114449467516 No Hit TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2657 0.16402662207019253 No Hit TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC 2573 0.1588409855425688 No Hit TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT 2365 0.14600036175988157 No Hit TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 2326 0.1435927448006277 No Hit TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT 2214 0.1366785627637961 No Hit TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT 2118 0.13075212101794045 No Hit TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC 2038 0.12581341956306075 No Hit TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG 2029 0.12525781564938676 No Hit TACTTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC 2022 0.12482567927208478 No Hit TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 1967 0.121430322021855 No Hit TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAA 1648 0.10173724997052214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0018520130455798932 0.0 0.0 0.0 0.0 8 0.011914617259897313 0.0 0.0 0.0 0.0 9 0.02148335132872676 0.0 0.0 0.0 0.0 10 0.0797600284963074 0.0 0.0 0.0 0.0 11 0.13075212101794045 0.0 0.0 0.0 0.0 12 0.16310061554740257 0.0 0.0 0.0 0.0 13 0.19446136978588877 0.0 0.0 0.0 0.0 14 0.22915574750641876 0.0 0.0 0.0 0.0 15 0.2810738465508418 0.0 0.0 0.0 0.0 16 0.33175727023154483 0.0 0.0 0.0 0.0 17 0.37490917419355635 0.0 0.0 0.0 0.0 18 0.4186784158374278 0.0 0.0 0.0 0.0 19 0.46127471588576535 0.0 0.0 0.0 0.0 20 0.5061551653569848 0.0 0.0 0.0 0.0 21 0.5462203809096965 0.0 0.0 0.0 0.0 22 0.589125349798964 0.0 0.0 0.0 0.0 23 0.6338205979656254 0.0 0.0 0.0 0.0 24 0.6828372099053066 0.0 0.0 0.0 0.0 25 0.727902860681084 0.0 0.0 0.0 0.0 26 0.7777837453753691 0.0 0.0 0.0 0.0 27 0.8257508832558883 0.0 0.0 0.0 0.0 28 0.8783480537503573 0.0 0.0 0.0 0.0 29 0.9110052171207494 0.0 0.0 0.0 0.0 30 0.9458847961458374 0.0 0.0 0.0 0.0 31 0.9728007190749318 0.0 0.0 0.0 0.0 32 1.0019390576587222 0.0 0.0 0.0 0.0 33 1.0320651365334883 0.0 0.0 0.0 0.0 34 1.0649692349766244 0.0 0.0 0.0 0.0 35 1.10805940517045 0.0 0.0 0.0 0.0 36 1.1381854840452164 0.0 0.0 0.0 0.0 37 1.1684967642245405 0.0 0.0 0.0 0.0 38 1.2004748561448866 0.0 0.0 0.0 0.0 39 1.2334406883562088 0.0 0.0 0.0 0.0 40 1.266159585494787 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACGC 20 6.3122244E-4 46.0 29 CTCGTTA 30 1.8618775E-6 46.0 12 AATTTCG 25 3.4177803E-5 46.0 14 GTCGATA 20 6.3122244E-4 46.0 28 CGAAATA 20 6.3122244E-4 46.0 15 TTTCGAA 20 6.3122244E-4 46.0 39 CGCATAC 20 6.3122244E-4 46.0 44 TCGATAG 20 6.3122244E-4 46.0 29 CCGTATA 20 6.3122244E-4 46.0 18 TTGCGAT 20 6.3122244E-4 46.0 32 TCGTACC 60 0.0 46.0 38 CTATTCG 40 5.6134013E-9 46.0 39 CGTAACA 50 1.6370905E-11 46.0 35 ATCGGTT 20 6.3122244E-4 46.0 21 GACGAAT 20 6.3122244E-4 46.0 43 CGATCTA 30 1.8618775E-6 46.0 41 TCGACAC 20 6.3122244E-4 46.0 42 GTTATAT 30 1.8618775E-6 46.0 13 ACGAATA 25 3.4177803E-5 46.0 27 GCATATA 25 3.4177803E-5 46.0 37 >>END_MODULE