FastQCFastQC Report
Fri 17 Jun 2016
SRR1527772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626800
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC56220.3455864273420211No Hit
TACTTCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC54330.3339685271699041No Hit
TACTTCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44780.27526432259650846No Hit
TACTTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG42510.2613105483157118No Hit
TACTTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC40430.24852471108925497No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24660.1515859355790509No Hit
TACTTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23050.14168920580280303No Hit
TACTTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT22740.13978362429309074No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT22630.13910745020899926No Hit
TACTTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT21090.1296410130317187No Hit
TACTTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT20270.12460044258667323No Hit
TACTTCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC19970.12275633144824195No Hit
TACTTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC19780.12158839439390214No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT18730.11513400540939267No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTACTTCGGGAAG18670.11476518318170642No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACAC206.312231E-446.0000049
ATGATAC206.312231E-446.00000441
CTATCGT206.312231E-446.00000441
ACGCAAT206.312231E-446.00000418
CGATTAC405.6134013E-946.00000439
TCGATAG206.312231E-446.00000429
CGGATTA206.312231E-446.0000046
TCGACAC206.312231E-446.00000442
TATCGTC206.312231E-446.00000442
AATCGTC351.01992555E-746.00000419
CGAAAAT301.861883E-646.035
ACCGAAT253.4177858E-546.041
TCTAACG301.861883E-646.039
CCTATAT501.6370905E-1146.025
CGATACG501.6370905E-1146.035
TCGGGAT157600.043.110415
CGGGATA22950.043.093686
TCGGGAG208150.042.8287285
CGGATAA700.042.7142876
TCGGAAT2050.042.6341485
Produced by FastQC (version 0.11.5)