FastQCFastQC Report
Fri 17 Jun 2016
SRR1527771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4888256
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG204620.4185950981290669No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC198480.4060343811780725No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA154330.31571587085455427No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC140960.288364602835858No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC123270.25217582712525694No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC104760.21430956152869246No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG104670.21412544678511108No Hit
ACAATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG103090.2108932101755718No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG102540.20976806452035246No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT91740.18767429529059035No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG58310.11928589664698412No Hit
ACAATAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA56600.11578771651893846No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC54290.11106210476701713No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT52480.10735935270165885No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC51380.1051090613912201No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC50810.1039430013485382No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC50750.10382025818615065No Hit
ACAATAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC50130.10255191217481245No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG49950.10218368268764975No Hit
ACAATAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC49250.10075167912646146No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC49140.10052664999541758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAT565500.042.7239655
ATAGGGA1722750.042.6591224
AATAGGG4887550.042.5762633
CAATAGG4984850.042.5744822
ACAATAG5031450.042.574131
TAGGGGG881450.042.512455
TAGGGGA753100.042.4984745
ATAGGGG2568100.042.448584
TAGGGAG531750.042.4071435
AGGGATA80100.042.3795286
ATAGGGT230700.042.3025554
ATAGGGC375050.042.2109074
AGGGATC153000.041.886286
TAGGGAC318650.041.8459135
TAGGGGT470600.041.8308565
TAGGGGC474600.041.8213235
AGGGATT199000.041.777896
TAGGGAA322800.041.6555145
TAGGGTG97400.041.2895285
AGGGGGT188900.041.123356
Produced by FastQC (version 0.11.5)