##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527771_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4888256
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.951702611319867	33.0	31.0	34.0	3.0	34.0
2	30.469647252517053	34.0	31.0	34.0	16.0	34.0
3	32.282551282093245	34.0	31.0	34.0	28.0	34.0
4	36.27985215995234	37.0	35.0	37.0	35.0	37.0
5	36.29467953396876	37.0	35.0	37.0	35.0	37.0
6	36.47203153844643	37.0	37.0	37.0	35.0	37.0
7	36.50428189521989	37.0	37.0	37.0	35.0	37.0
8	36.68080067819689	37.0	37.0	37.0	35.0	37.0
9	38.77075014074549	39.0	39.0	39.0	39.0	39.0
10	38.37368521615889	39.0	39.0	39.0	37.0	39.0
11	38.18246753034211	39.0	39.0	39.0	37.0	39.0
12	37.87320672239752	39.0	38.0	39.0	35.0	39.0
13	37.79793447806334	39.0	38.0	39.0	35.0	39.0
14	38.563115761531314	40.0	39.0	40.0	35.0	40.0
15	38.633622298013854	40.0	39.0	40.0	35.0	40.0
16	38.67722864759947	40.0	39.0	40.0	35.0	40.0
17	38.6310923568651	40.0	39.0	40.0	35.0	40.0
18	38.57763914164888	40.0	38.0	40.0	35.0	40.0
19	38.59144733827361	40.0	39.0	40.0	35.0	40.0
20	38.48801167532961	40.0	38.0	40.0	35.0	40.0
21	38.49024969232381	40.0	38.0	40.0	35.0	40.0
22	38.49135315335367	40.0	38.0	40.0	35.0	40.0
23	38.425188860812526	40.0	38.0	40.0	35.0	40.0
24	38.36463372622056	40.0	38.0	40.0	35.0	40.0
25	38.33409072683591	40.0	38.0	40.0	35.0	40.0
26	38.23735377197921	40.0	38.0	40.0	35.0	40.0
27	38.128566098011234	40.0	38.0	40.0	35.0	40.0
28	37.98912863810733	40.0	38.0	40.0	34.0	40.0
29	37.9587233156365	40.0	38.0	40.0	34.0	40.0
30	38.13487550570183	40.0	38.0	40.0	35.0	40.0
31	38.17495196650912	40.0	38.0	40.0	35.0	40.0
32	38.17724358135089	40.0	38.0	40.0	35.0	40.0
33	38.08109252052266	40.0	38.0	40.0	35.0	40.0
34	37.816082668338154	40.0	38.0	40.0	34.0	40.0
35	37.79522267246232	40.0	37.0	40.0	34.0	40.0
36	37.770041094410765	40.0	37.0	40.0	34.0	40.0
37	37.66489070948821	40.0	37.0	40.0	34.0	40.0
38	37.590476849003	40.0	37.0	40.0	33.0	40.0
39	37.48172047454143	40.0	36.0	40.0	33.0	40.0
40	37.28784417182734	40.0	36.0	40.0	33.0	40.0
41	37.16610566222391	40.0	35.0	40.0	33.0	40.0
42	37.09106397046308	40.0	35.0	40.0	33.0	40.0
43	36.98128535003077	40.0	35.0	40.0	33.0	40.0
44	36.631497409300984	40.0	35.0	40.0	31.0	40.0
45	36.63466909261708	39.0	35.0	40.0	31.0	40.0
46	36.47252455681536	39.0	35.0	40.0	31.0	40.0
47	36.424781762657275	39.0	35.0	40.0	31.0	40.0
48	36.36822416829233	39.0	35.0	40.0	31.0	40.0
49	36.28661285333665	39.0	35.0	40.0	31.0	40.0
50	35.84362398368661	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	6.0
15	31.0
16	58.0
17	169.0
18	390.0
19	728.0
20	1248.0
21	2152.0
22	3504.0
23	5503.0
24	8168.0
25	11712.0
26	16070.0
27	21815.0
28	29954.0
29	41209.0
30	55734.0
31	74117.0
32	91823.0
33	129890.0
34	221681.0
35	303237.0
36	391061.0
37	881428.0
38	1482631.0
39	1113932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.60255764018906	0.31808890532738054	0.05871214600871968	0.02064130847484256
8	98.97343756137158	0.9248901857840506	0.06581079223346732	0.0358614606109009
9	97.87005836028227	1.0808149164037235	0.441077554039723	0.608049169274277
10	52.10766375574438	35.12334460388327	4.928649399704107	7.840342240668247
11	32.69613948205659	25.644360688147266	24.003305064219223	17.656194765576927
12	27.584152712132916	19.31222505531625	27.447232714489584	25.656389518061246
13	24.840024745021537	19.09646303303264	29.981347130755836	26.082165091189985
14	25.043307879129078	20.25755197763783	29.826568002985116	24.87257214024797
15	25.64043290695086	20.355787421935347	28.034538289320366	25.969241381793424
16	28.864241152672854	21.751602207413033	25.305221330470417	24.078935309443693
17	28.775170531166943	22.051893354194217	24.19998052475157	24.97295558988727
18	27.651436422315033	21.598561941109466	24.794548403356945	25.955453233218556
19	27.639857650663142	22.379106167925737	24.330517877950747	25.650518303460373
20	26.63708692834418	23.23800144673274	24.48601300750206	25.63889861742102
21	27.361251129237097	22.043587733539326	26.070177175663467	24.524983961560114
22	27.148107627751084	21.51358275834981	25.618502795270953	25.719806818628154
23	25.89944143678236	22.52058811977114	25.940580853375927	25.63938959007057
24	26.663517622644967	23.08520666675395	24.26243633721311	25.98883937338797
25	26.4950321750743	23.723777969075268	23.95623306144359	25.824956794406837
26	25.71549035075086	24.034747770984172	23.938844446772016	26.310917431492946
27	25.85171480380733	22.398479130389244	24.332461311355218	27.417344754448216
28	27.173720034302622	23.394642179133008	23.370277661399076	26.061360125165294
29	27.153140097408972	23.57354033831289	24.147425994055958	25.12589357022218
30	27.044369198339858	22.852833403160556	24.252657798609565	25.85013959989002
31	28.607728400476574	23.05893963000301	23.72120036266513	24.612131606855286
32	29.44064304324487	23.49117967635083	23.45615696068291	23.61202031972139
33	29.369963438903362	22.586869427460428	24.309590168763666	23.733576964872544
34	28.387854482252976	23.40345922963118	25.729319413713192	22.47936687440265
35	27.361455701174407	24.908147200146637	25.491074935518927	22.23932216316003
36	27.84663896489873	24.3816608622789	24.153092636719517	23.618607536102857
37	27.69273949645845	25.269012097566083	22.827732426452297	24.210515979523166
38	28.04061407585855	25.30593733225101	23.013749689050655	23.639698902839786
39	27.607126140693122	23.550444166590292	22.808564035926103	26.03386565679048
40	27.34335108472224	24.426257544613048	22.835547074457637	25.394844296207076
41	25.406484439440156	24.857679303211615	23.74198487149609	25.993851385852135
42	24.999938628418803	23.115933371738304	24.764067184697364	27.120060815145525
43	24.85528581154506	23.179002900011785	26.11837432409432	25.847336964348838
44	26.157468021314763	22.660965383155055	25.26923712669713	25.912329468833057
45	26.061073724453053	23.256965265321618	24.792625427146206	25.88933558307912
46	26.331661025936448	24.534721585776197	23.37942202699695	25.754195361290407
47	25.672121100040584	24.519460519252675	24.972976047081005	24.835442333625736
48	26.254066890113776	24.481573796462378	24.525761334921903	24.738597978501943
49	26.708707563597322	23.893511305463544	24.11892912318831	25.278852007750825
50	24.915614075858546	24.633795775016694	24.411385164770422	26.039204984354335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	26.5
2	53.0
3	56.5
4	60.0
5	157.5
6	255.0
7	2644.0
8	5033.0
9	10129.0
10	15225.0
11	16697.0
12	18169.0
13	17826.0
14	17483.0
15	16304.5
16	15126.0
17	16129.0
18	17132.0
19	17496.5
20	17861.0
21	20711.5
22	23562.0
23	29601.0
24	35640.0
25	40148.0
26	44656.0
27	49229.0
28	53802.0
29	61059.0
30	68316.0
31	77519.5
32	86723.0
33	95968.5
34	105214.0
35	115565.0
36	125916.0
37	130410.5
38	134905.0
39	143570.0
40	152235.0
41	167910.0
42	183585.0
43	193852.0
44	204119.0
45	224824.5
46	245530.0
47	258249.5
48	270969.0
49	276817.0
50	282665.0
51	297678.0
52	312691.0
53	344633.0
54	376575.0
55	401407.0
56	426239.0
57	413659.0
58	401079.0
59	389276.5
60	377474.0
61	334215.0
62	290956.0
63	238648.5
64	186341.0
65	160385.0
66	134429.0
67	114824.5
68	95220.0
69	79392.5
70	63565.0
71	59888.0
72	56211.0
73	41370.5
74	26530.0
75	18006.5
76	9483.0
77	7220.0
78	4957.0
79	3309.5
80	1662.0
81	1074.5
82	487.0
83	274.5
84	62.0
85	49.5
86	37.0
87	20.0
88	3.0
89	12.0
90	21.0
91	10.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4888256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.044340545428271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.79413874563507	4.352989570356971
2	10.329400282544418	1.4552762644057176
3	4.39850119791809	0.9295362098282767
4	2.1211514632399924	0.5976845302198392
5	1.1549717046023598	0.4068007003776403
6	0.73517598957143	0.3107298018818026
7	0.5566264427222501	0.2744746353388098
8	0.4123231398285356	0.2323635689369955
9	0.3550022661847047	0.22506811716634512
>10	9.986569629894683	20.78929052249077
>50	5.435221648589122	26.50101621759703
>100	2.5937414446902354	31.587134992797356
>500	0.08587354412008451	4.003121664515684
>1k	0.03595397521982777	4.664768718682739
>5k	0.002674262619656611	1.081094865570205
>10k+	0.002674262619656611	2.5886496198338476
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	20462	0.4185950981290669	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	19848	0.4060343811780725	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	15433	0.31571587085455427	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	14096	0.288364602835858	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	12327	0.25217582712525694	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	10476	0.21430956152869246	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	10467	0.21412544678511108	No Hit
ACAATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	10309	0.2108932101755718	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	10254	0.20976806452035246	No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	9174	0.18767429529059035	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	5831	0.11928589664698412	No Hit
ACAATAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA	5660	0.11578771651893846	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	5429	0.11106210476701713	No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	5248	0.10735935270165885	No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	5138	0.1051090613912201	No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	5081	0.1039430013485382	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5075	0.10382025818615065	No Hit
ACAATAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTC	5013	0.10255191217481245	No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	4995	0.10218368268764975	No Hit
ACAATAGGGGAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	4925	0.10075167912646146	No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	4914	0.10052664999541758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.05134755626546564	0.0	0.0	0.0	0.0
7	0.053536475994710585	0.0	0.0	0.0	0.0
8	0.06822474109375613	0.0	0.0	0.0	0.0
9	0.08847736288770473	0.0	0.0	0.0	0.0
10	0.12354099294308644	0.0	0.0	0.0	0.0
11	0.14880562720119406	0.0	0.0	0.0	0.0
12	0.16187777399546996	0.0	0.0	0.0	0.0
13	0.17139036908050642	0.0	0.0	0.0	9.205737179067545E-4
14	0.1833782846070255	0.0	0.0	0.0	9.205737179067545E-4
15	0.19602083033294493	0.0	0.0	0.0	9.205737179067545E-4
16	0.2102590371699027	0.0	0.0	0.0	9.205737179067545E-4
17	0.22349484151402874	0.0	0.0	0.0	9.205737179067545E-4
18	0.2356464145903979	0.0	0.0	0.0	9.205737179067545E-4
19	0.25195079799421305	0.0	0.0	0.0	9.205737179067545E-4
20	0.2628953966404378	0.0	0.0	0.0	9.205737179067545E-4
21	0.2740241100302439	0.0	0.0	0.0	9.205737179067545E-4
22	0.2845186504143809	0.0	0.0	0.0	9.205737179067545E-4
23	0.29673159507194385	0.0	0.0	0.0	9.205737179067545E-4
24	0.3093741407978633	0.0	0.0	0.0	9.205737179067545E-4
25	0.32109611280587597	0.0	0.0	0.0	9.205737179067545E-4
26	0.334413745924927	0.0	0.0	0.0	9.205737179067545E-4
27	0.34713812042577147	0.0	0.0	0.0	9.205737179067545E-4
28	0.36301290276123016	0.0	0.0	0.0	9.205737179067545E-4
29	0.3749394467065555	0.0	0.0	0.0	9.205737179067545E-4
30	0.38874805247515676	0.0	0.0	0.0	9.205737179067545E-4
31	0.400122252189738	0.0	0.0	0.0	9.205737179067545E-4
32	0.41315348459655143	0.0	0.0	0.0	9.205737179067545E-4
33	0.4249777425732204	0.0	0.0	0.0	9.205737179067545E-4
34	0.4381112609486901	0.0	0.0	0.0	9.205737179067545E-4
35	0.4531882127286296	0.0	0.0	0.0	9.205737179067545E-4
36	0.46501247070529855	0.0	0.0	0.0	9.205737179067545E-4
37	0.47753227326883046	0.0	0.0	0.0	9.205737179067545E-4
38	0.49023619057594364	0.0	0.0	0.0	9.205737179067545E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGAT	56550	0.0	42.723965	5
ATAGGGA	172275	0.0	42.659122	4
AATAGGG	488755	0.0	42.576263	3
CAATAGG	498485	0.0	42.574482	2
ACAATAG	503145	0.0	42.57413	1
TAGGGGG	88145	0.0	42.51245	5
TAGGGGA	75310	0.0	42.498474	5
ATAGGGG	256810	0.0	42.44858	4
TAGGGAG	53175	0.0	42.407143	5
AGGGATA	8010	0.0	42.379528	6
ATAGGGT	23070	0.0	42.302555	4
ATAGGGC	37505	0.0	42.210907	4
AGGGATC	15300	0.0	41.88628	6
TAGGGAC	31865	0.0	41.845913	5
TAGGGGT	47060	0.0	41.830856	5
TAGGGGC	47460	0.0	41.821323	5
AGGGATT	19900	0.0	41.77789	6
TAGGGAA	32280	0.0	41.655514	5
TAGGGTG	9740	0.0	41.289528	5
AGGGGGT	18890	0.0	41.12335	6
>>END_MODULE