FastQCFastQC Report
Fri 17 Jun 2016
SRR1527770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6136241
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG251970.41062598421411417No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA244390.3982731447477373No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC194920.31765375577654137No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC171120.27886779544675644No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA153630.25036500359096064No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC133920.21824436165398328No Hit
ACAATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC131830.21483836765863662No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT129410.21089458513770892No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT115820.18874747585696194No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG115440.18812820422144438No Hit
ACAATAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC73480.11974757836271425No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC69810.11376671809337345No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC69160.11270743766419865No Hit
ACAATAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT67300.10967626597456No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT64950.10584655980754341No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC64770.10555322061177193No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG64760.10553692398978462No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG64740.10550433074581No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT64070.10441245707266061No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC62490.10183759079866647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGA2151200.044.5780034
TAGGGAT712650.044.528315
CAATAGG6268150.044.4662132
ACAATAG6326950.044.461931
AATAGGG6140900.044.4587753
ATAGGGT287350.044.311124
TAGGGGG1126050.044.298575
TAGGGAG662550.044.2746965
ATAGGGG3251500.044.2520984
TAGGGGA946850.044.1514475
ATAGGGC464600.044.128714
AGGGATA101250.044.0691386
TAGGGGT588250.043.7752655
AGGGATT247350.043.6381656
TAGGGGC607450.043.5388955
TAGGGAC399950.043.5329445
AGGGATC197500.043.3797456
TAGGGAA403450.043.1723865
TAGGGTG125900.042.9857035
AGGGGGT246500.042.6409726
Produced by FastQC (version 0.11.5)