##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6136241
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25228881981656	33.0	31.0	34.0	31.0	34.0
2	32.78368809178127	34.0	31.0	34.0	31.0	34.0
3	32.860523242160795	34.0	31.0	34.0	31.0	34.0
4	36.44631819382583	37.0	37.0	37.0	35.0	37.0
5	36.26024890482626	37.0	37.0	37.0	35.0	37.0
6	36.29497993967316	37.0	37.0	37.0	35.0	37.0
7	36.40914559907279	37.0	36.0	37.0	35.0	37.0
8	36.6030933270059	37.0	37.0	37.0	35.0	37.0
9	38.70008381352688	39.0	39.0	39.0	39.0	39.0
10	38.12312668293178	39.0	39.0	39.0	37.0	39.0
11	37.83163731672208	39.0	38.0	39.0	35.0	39.0
12	37.51411849697559	39.0	37.0	39.0	35.0	39.0
13	37.437733948194015	39.0	37.0	39.0	35.0	39.0
14	38.22411750125199	40.0	38.0	40.0	35.0	40.0
15	38.30250131961896	40.0	38.0	40.0	35.0	40.0
16	38.34681672378904	40.0	38.0	40.0	35.0	40.0
17	38.29430330392825	40.0	38.0	40.0	35.0	40.0
18	38.28499923650326	40.0	38.0	40.0	35.0	40.0
19	38.30844909774567	40.0	38.0	40.0	35.0	40.0
20	38.26127412531548	40.0	38.0	40.0	34.0	40.0
21	38.24028798738511	40.0	38.0	40.0	34.0	40.0
22	38.23887376652905	40.0	38.0	40.0	34.0	40.0
23	38.20367599642843	40.0	38.0	40.0	34.0	40.0
24	38.1490317932428	40.0	38.0	40.0	34.0	40.0
25	38.12399757441079	40.0	38.0	40.0	34.0	40.0
26	38.01765152313933	40.0	38.0	40.0	34.0	40.0
27	37.921236144408276	40.0	38.0	40.0	34.0	40.0
28	37.81480355807407	40.0	37.0	40.0	34.0	40.0
29	37.73754436959044	40.0	37.0	40.0	34.0	40.0
30	37.64607974165291	40.0	37.0	40.0	33.0	40.0
31	37.561042827359614	40.0	37.0	40.0	33.0	40.0
32	37.47378060933396	40.0	37.0	40.0	33.0	40.0
33	37.340837004283244	40.0	36.0	40.0	33.0	40.0
34	37.23089216997833	40.0	36.0	40.0	33.0	40.0
35	37.11129875765962	40.0	36.0	40.0	32.0	40.0
36	37.08757397240428	39.0	35.0	40.0	32.0	40.0
37	36.97295624471073	39.0	35.0	40.0	32.0	40.0
38	36.89169851705629	39.0	35.0	40.0	32.0	40.0
39	36.78624209837912	39.0	35.0	40.0	31.0	40.0
40	36.56486829640492	39.0	35.0	40.0	31.0	40.0
41	36.43725515344003	39.0	35.0	40.0	31.0	40.0
42	36.373956792114264	39.0	35.0	40.0	31.0	40.0
43	36.237731210361524	38.0	35.0	40.0	30.0	40.0
44	36.104403656896785	38.0	35.0	40.0	30.0	40.0
45	35.99889671869146	38.0	35.0	40.0	30.0	40.0
46	35.89247097693849	38.0	35.0	40.0	30.0	40.0
47	35.71238629643132	38.0	35.0	40.0	29.0	40.0
48	35.53972440130693	38.0	34.0	40.0	29.0	40.0
49	35.81350276170704	38.0	35.0	40.0	30.0	40.0
50	35.85883409729181	38.0	35.0	40.0	30.0	40.0
51	35.83599861869832	38.0	35.0	40.0	30.0	40.0
52	35.38685019053196	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	13.0
15	24.0
16	88.0
17	225.0
18	529.0
19	1081.0
20	2002.0
21	3562.0
22	6103.0
23	9765.0
24	14500.0
25	20732.0
26	28557.0
27	39448.0
28	52236.0
29	68165.0
30	88694.0
31	113169.0
32	147958.0
33	196404.0
34	331193.0
35	396483.0
36	543565.0
37	905053.0
38	2053277.0
39	1113409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.65808057408437	0.30510535684631684	0.025243467458334834	0.01157060161098627
8	99.0084972216704	0.9118448900556545	0.050763977490453846	0.028893910783491066
9	97.87576791719881	1.0870987629071283	0.42980710829317165	0.6073262116008807
10	52.16944706050496	35.12508716655686	4.879338995974898	7.8261267769632905
11	32.626521676707284	25.66084024405169	23.93414795800882	17.778490121232203
12	27.602566457217048	19.252975885399547	27.459710268876336	25.684747388507066
13	24.836785256641647	19.006228731889767	29.956222384355502	26.200763627113083
14	25.200672528996172	20.207403848708026	29.702500276635156	24.889423345660642
15	25.702852935534963	20.250084701692778	27.966747068767344	26.08031529400491
16	28.895410072713894	21.648220791849603	25.295926284511967	24.160442850924532
17	28.822759731894493	21.924611500754292	24.203889645142688	25.04873912220853
18	27.72415555386433	21.49472290935118	24.800411196365985	25.980710340418504
19	27.70649001563009	22.24699127690715	24.354959330965002	25.691559376497764
20	26.619228286503088	23.14302844363512	24.48363745817676	25.754105811685037
21	27.49522060818667	21.889573763481586	26.070455837702593	24.54474979062915
22	27.18314029712979	21.41661320016603	25.59421965336759	25.80602684933659
23	25.984034851303917	22.389472642942152	25.943407372689563	25.683085133064367
24	26.706627070221	22.93539318289487	24.26069966939043	26.097280077493696
25	26.521171512005477	23.575165968872476	23.940959945999513	25.962702573122538
26	25.759304434098983	23.954078726699294	23.941236988573298	26.34537985062842
27	25.929261904804584	22.222464860816256	24.29092990317688	27.557343331202276
28	27.26876274905109	23.326984712627812	23.341716858904334	26.062535679416765
29	27.18703518978476	23.373641941377464	24.16614666862009	25.27317620021769
30	27.12134350655393	22.68701636718636	24.27290583925892	25.918734287000788
31	28.714713128118664	22.92095437581412	23.680539926642385	24.68379256942483
32	29.51754991370124	23.311910337289557	23.492916917702548	23.677622831306657
33	29.463445128703388	22.421251055817397	24.319237135568827	23.79606667991039
34	28.30423381350244	23.29954120120119	25.7550510157603	22.641173969536073
35	27.4333097412569	24.828506572672097	25.452911644115673	22.285272041955327
36	27.968539697185946	24.24650531163949	24.12858295493935	23.65637203623521
37	27.78399674980171	25.095803766507867	22.837662340837003	24.282537142853418
38	28.13276075695202	25.182518092102313	22.97602066150922	23.70870048943645
39	27.665373638356122	23.414872395005347	22.792243655358384	26.127510311280144
40	27.42667701610807	24.290718047091044	22.80164680624506	25.480958130555827
41	25.47069125870382	24.756084384560513	23.715007282145535	26.05821707459013
42	25.04891838505039	22.91150233506148	24.77647145866663	27.263107821221492
43	24.957119513395902	23.033401067526523	26.06087342397406	25.94860599510352
44	26.02357045624512	22.50574904082157	25.36398097793095	26.10669952500236
45	26.07081436338631	23.097349012204702	24.881226144801026	25.950610479607956
46	26.177215008341427	24.324109825543033	23.47235058075457	26.02632458536097
47	25.708589346474497	24.331573678413218	24.99262985270624	24.967207122406045
48	26.379733129777662	24.250220941452593	24.586045430744978	24.78400049802477
49	26.78936502005055	23.633507875587025	24.237020677642874	25.34010642671955
50	24.905654781159996	24.48868941099282	24.493806550296835	26.111849257550347
51	24.92219585247711	24.55192030430356	24.153174557518195	26.372709285701134
52	25.441275856016738	24.71314278562397	24.26777240333292	25.577808955026377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	33.5
2	67.0
3	72.0
4	77.0
5	171.0
6	265.0
7	2987.0
8	5709.0
9	11456.0
10	17203.0
11	18776.0
12	20349.0
13	20128.0
14	18589.0
15	17271.0
16	17580.5
17	17890.0
18	18853.5
19	19817.0
20	22338.5
21	24860.0
22	29436.5
23	34013.0
24	40701.0
25	47389.0
26	51656.0
27	55923.0
28	63028.0
29	70133.0
30	80909.0
31	91685.0
32	100382.5
33	109080.0
34	122767.0
35	136454.0
36	146367.5
37	156281.0
38	161033.5
39	178689.5
40	191593.0
41	206935.5
42	222278.0
43	242894.0
44	263510.0
45	273431.5
46	283353.0
47	300283.0
48	317213.0
49	332734.5
50	348256.0
51	361795.0
52	375334.0
53	419280.5
54	463227.0
55	482388.0
56	501549.0
57	487231.0
58	472913.0
59	470040.5
60	467168.0
61	421638.5
62	376109.0
63	330265.0
64	233546.5
65	182672.0
66	155036.5
67	127401.0
68	112617.0
69	97833.0
70	87973.0
71	78113.0
72	61015.0
73	43917.0
74	30392.0
75	16867.0
76	12460.5
77	8054.0
78	5629.5
79	3205.0
80	2024.0
81	843.0
82	524.5
83	206.0
84	117.0
85	28.0
86	18.0
87	8.0
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	6136241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.777280953349533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.9214831671782	5.282444573593459
2	9.594459430019718	1.4923761316555433
3	4.20504841632058	0.98111528868488
4	2.06274436592233	0.6417016987486719
5	1.160880995429722	0.45142488274305104
6	0.665043590836492	0.3103338511295898
7	0.4760606295767084	0.2591720086932565
8	0.3475273104952212	0.21622540261466183
9	0.2707589746532432	0.18951917548672056
>10	5.787529318910419	13.21081307872371
>50	4.6304809261739726	25.606030506647304
>100	2.741318387437135	37.28981511622596
>500	0.09570693435348904	4.962535650320773
>1k	0.035942342158952215	4.987563667027811
>5k	0.0029255394780542503	1.4496259780634093
>10k+	0.002089671055753036	2.6693029896411837
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	25197	0.41062598421411417	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	24439	0.3982731447477373	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	19492	0.31765375577654137	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17112	0.27886779544675644	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	15363	0.25036500359096064	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	13392	0.21824436165398328	No Hit
ACAATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	13183	0.21483836765863662	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	12941	0.21089458513770892	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11582	0.18874747585696194	No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	11544	0.18812820422144438	No Hit
ACAATAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	7348	0.11974757836271425	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	6981	0.11376671809337345	No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	6916	0.11270743766419865	No Hit
ACAATAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	6730	0.10967626597456	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	6495	0.10584655980754341	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6477	0.10555322061177193	No Hit
ACAATAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	6476	0.10553692398978462	No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	6474	0.10550433074581	No Hit
ACAATAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	6407	0.10441245707266061	No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	6249	0.10183759079866647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.045483871966567155	0.0	0.0	0.0	0.0
7	0.04760243282491675	0.0	0.0	0.0	0.0
8	0.06080269663463348	0.0	0.0	0.0	0.0
9	0.07871268419868124	0.0	0.0	0.0	0.0
10	0.11078443626969671	0.0	0.0	0.0	0.0
11	0.13425157193141535	0.0	0.0	0.0	0.0
12	0.14596884314028735	0.0	0.0	0.0	0.0
13	0.15447567981766036	0.0	0.0	0.0	8.474243433398395E-4
14	0.16402550030222085	0.0	0.0	0.0	8.474243433398395E-4
15	0.1754494323153214	0.0	0.0	0.0	8.474243433398395E-4
16	0.187948941379584	0.0	0.0	0.0	8.474243433398395E-4
17	0.1996173227224941	0.0	0.0	0.0	8.474243433398395E-4
18	0.21151385677322648	0.0	0.0	0.0	8.474243433398395E-4
19	0.22657193548949592	0.0	0.0	0.0	8.474243433398395E-4
20	0.23657806138970097	0.0	0.0	0.0	8.474243433398395E-4
21	0.24617677174022337	0.0	0.0	0.0	8.474243433398395E-4
22	0.2559058550666442	0.0	0.0	0.0	8.474243433398395E-4
23	0.2669386681520494	0.0	0.0	0.0	8.474243433398395E-4
24	0.27839519340912455	0.0	0.0	0.0	8.474243433398395E-4
25	0.2890531841888218	0.0	0.0	0.0	8.474243433398395E-4
26	0.3017319560949448	0.0	0.0	0.0	8.637209653271441E-4
27	0.3135633036577279	0.0	0.0	0.0	8.637209653271441E-4
28	0.32738283910296223	0.0	0.0	0.0	8.637209653271441E-4
29	0.33877417787208813	0.0	0.0	0.0	8.637209653271441E-4
30	0.35058922881288396	0.0	0.0	0.0	8.637209653271441E-4
31	0.36139388919046694	0.0	0.0	0.0	8.637209653271441E-4
32	0.3736652455469073	0.0	0.0	0.0	8.637209653271441E-4
33	0.3849099147181475	0.0	0.0	0.0	8.637209653271441E-4
34	0.39757239000228317	0.0	0.0	0.0	8.637209653271441E-4
35	0.41129414571559364	0.0	0.0	0.0	8.637209653271441E-4
36	0.42296252705850373	0.0	0.0	0.0	8.637209653271441E-4
37	0.4346146117794265	0.0	0.0	0.0	8.637209653271441E-4
38	0.4454518654009841	0.0	0.0	0.0	8.637209653271441E-4
39	0.4561750426686305	0.0	0.0	0.0	8.637209653271441E-4
40	0.4685115855130201	0.0	0.0	0.0	8.637209653271441E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGGA	215120	0.0	44.578003	4
TAGGGAT	71265	0.0	44.52831	5
CAATAGG	626815	0.0	44.466213	2
ACAATAG	632695	0.0	44.46193	1
AATAGGG	614090	0.0	44.458775	3
ATAGGGT	28735	0.0	44.31112	4
TAGGGGG	112605	0.0	44.29857	5
TAGGGAG	66255	0.0	44.274696	5
ATAGGGG	325150	0.0	44.252098	4
TAGGGGA	94685	0.0	44.151447	5
ATAGGGC	46460	0.0	44.12871	4
AGGGATA	10125	0.0	44.069138	6
TAGGGGT	58825	0.0	43.775265	5
AGGGATT	24735	0.0	43.638165	6
TAGGGGC	60745	0.0	43.538895	5
TAGGGAC	39995	0.0	43.532944	5
AGGGATC	19750	0.0	43.379745	6
TAGGGAA	40345	0.0	43.172386	5
TAGGGTG	12590	0.0	42.985703	5
AGGGGGT	24650	0.0	42.640972	6
>>END_MODULE