FastQCFastQC Report
Fri 17 Jun 2016
SRR1527769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175714
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG239760.38823041351979704No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA231870.37545456282463857No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC187810.3041105854319031No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC162080.2624473866503533No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA147930.23953505618945437No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC128440.20797595225426568No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT127640.20668055547909117No Hit
ACAATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC126090.2041707242271906No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT112740.18255379054146614No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG110050.17819801888494188No Hit
ACAATAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC69790.11300717617428527No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC65500.10606061096741203No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC64510.10445755745813359No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG63430.10270877181164802No Hit
ACAATAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT63220.10236873015816471No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC62350.10095998616516243No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC62140.10061994451167915No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT61980.10036086515664423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAT708450.044.6689265
ATAGGGA2158350.044.6637044
CAATAGG6302800.044.5020142
AATAGGG6172400.044.5009233
ACAATAG6363150.044.4970661
ATAGGGG3257800.044.2872474
ATAGGGT287750.044.281494
TAGGGGG1125000.044.274495
TAGGGAG670950.044.2723055
TAGGGGA951200.044.2662965
AGGGATA101750.044.101236
ATAGGGC483100.044.0004124
TAGGGGT601500.043.8472185
TAGGGAC403050.043.6717535
AGGGATT256800.043.5369956
TAGGGGC597400.043.5051885
AGGGATC186450.043.409496
TAGGGAA404850.043.0969475
TAGGGTA75600.042.9576725
TAGGGTG119300.042.7803885
Produced by FastQC (version 0.11.5)