##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527769_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6175714
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.210599454573185	33.0	31.0	34.0	31.0	34.0
2	32.756005054638216	34.0	31.0	34.0	31.0	34.0
3	32.85664669704588	34.0	31.0	34.0	31.0	34.0
4	36.42502130118072	37.0	37.0	37.0	35.0	37.0
5	36.26294174244468	37.0	37.0	37.0	35.0	37.0
6	36.2234923767519	37.0	37.0	37.0	35.0	37.0
7	36.35711271603575	37.0	36.0	37.0	35.0	37.0
8	36.56913759283542	37.0	37.0	37.0	35.0	37.0
9	38.65344233881297	39.0	39.0	39.0	38.0	39.0
10	38.03132334172211	39.0	38.0	39.0	37.0	39.0
11	37.79592222049143	39.0	38.0	39.0	35.0	39.0
12	37.46514961670829	39.0	37.0	39.0	35.0	39.0
13	37.242729018863244	39.0	37.0	39.0	34.0	39.0
14	38.162563065582376	40.0	38.0	40.0	34.0	40.0
15	38.256222519371846	40.0	38.0	40.0	35.0	40.0
16	38.33074394312949	40.0	38.0	40.0	35.0	40.0
17	38.30837519354037	40.0	38.0	40.0	35.0	40.0
18	38.30738955851906	40.0	38.0	40.0	35.0	40.0
19	38.29025502152464	40.0	38.0	40.0	34.0	40.0
20	38.25483644482241	40.0	38.0	40.0	34.0	40.0
21	38.28778631912035	40.0	38.0	40.0	35.0	40.0
22	38.173860058934075	40.0	38.0	40.0	34.0	40.0
23	38.17315843965572	40.0	38.0	40.0	34.0	40.0
24	38.16475390537839	40.0	38.0	40.0	34.0	40.0
25	38.10934444179248	40.0	38.0	40.0	34.0	40.0
26	37.94950851027104	40.0	38.0	40.0	34.0	40.0
27	37.86310424997013	40.0	38.0	40.0	34.0	40.0
28	37.771684213355734	40.0	37.0	40.0	34.0	40.0
29	37.69150579188091	40.0	37.0	40.0	33.0	40.0
30	37.5946076842289	40.0	37.0	40.0	33.0	40.0
31	37.53109243724693	40.0	37.0	40.0	33.0	40.0
32	37.4469068677727	40.0	37.0	40.0	33.0	40.0
33	37.129789689095055	40.0	36.0	40.0	32.0	40.0
34	37.13489873397635	40.0	36.0	40.0	32.0	40.0
35	37.088725611322026	39.0	36.0	40.0	32.0	40.0
36	36.912795670265815	39.0	35.0	40.0	32.0	40.0
37	36.88247690874286	39.0	35.0	40.0	31.0	40.0
38	36.52287719282337	39.0	35.0	40.0	31.0	40.0
39	36.608276063302156	39.0	35.0	40.0	31.0	40.0
40	36.46226946390328	39.0	35.0	40.0	31.0	40.0
41	36.13693720272668	39.0	35.0	40.0	30.0	40.0
42	36.21479864514451	39.0	35.0	40.0	30.0	40.0
43	36.18682649487978	38.0	35.0	40.0	30.0	40.0
44	36.047909763956035	38.0	35.0	40.0	30.0	40.0
45	35.943863980747814	38.0	35.0	40.0	30.0	40.0
46	35.662197925616375	38.0	35.0	40.0	29.0	40.0
47	35.39787690945533	38.0	34.0	40.0	28.0	40.0
48	35.39727519765326	38.0	34.0	40.0	28.0	40.0
49	35.32635627232738	37.0	34.0	40.0	28.0	40.0
50	35.16982052601529	37.0	34.0	40.0	28.0	40.0
51	35.46182692397996	37.0	34.0	40.0	29.0	40.0
52	35.214393024029285	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	2.0
14	12.0
15	20.0
16	81.0
17	222.0
18	600.0
19	1216.0
20	2211.0
21	3918.0
22	6463.0
23	10423.0
24	15388.0
25	22041.0
26	30097.0
27	41011.0
28	54980.0
29	71284.0
30	92649.0
31	118686.0
32	156057.0
33	210391.0
34	344742.0
35	412165.0
36	582081.0
37	975898.0
38	2060176.0
39	962896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.65058291235637	0.305082133013284	0.03183437574991329	0.012500578880433906
8	99.00701360199	0.9115707106902942	0.05246356939456717	0.02895211792515003
9	97.84478361530343	1.0920194814721018	0.45071711546227694	0.6124797877621924
10	52.16146667413679	35.09220148471901	4.912597960333008	7.833733880811191
11	32.655543958156095	25.68193734360108	23.91365597564913	17.748862722593696
12	27.623720917127965	19.238698553721886	27.46440330624119	25.67317722290896
13	25.082767757703806	19.01702378057015	29.846589398408025	26.053619063318024
14	25.206154300539175	20.192677316339456	29.709811691409282	24.891356691712083
15	25.678796006421283	20.25288088146569	28.013214342503556	26.055108769609475
16	28.91510196229942	21.643813168809306	25.299633370327708	24.14145149856357
17	28.82374410473024	21.922760671883445	24.20333907949753	25.050156143888785
18	27.749811600731512	21.451479132615273	24.79914063377935	25.999568632873864
19	27.732032279992243	22.238319326315953	24.30533538308283	25.724313010608974
20	26.615950803421274	23.133114648767737	24.499078163269868	25.75185638454112
21	27.48621454944319	21.866993840712183	26.054121029568407	24.592670580276224
22	27.37186339911466	21.370403486949037	25.559797620161817	25.697935493774487
23	26.026059496926184	22.382917991344804	25.93139837758031	25.6596241341487
24	26.670292698139843	22.902631177544816	24.280334225321962	26.14674189899338
25	26.565041710156915	23.55967261437301	23.938819058006896	25.93646661746318
26	25.824949147580345	23.915858150166926	23.96148850157245	26.29770420068028
27	25.97808123886566	22.259466678670677	24.23076910621185	27.531682976251815
28	27.23369314058261	23.294812551228894	23.39408852158633	26.077405786602164
29	27.20168064777611	23.363598119990662	24.219110535235277	25.21561069699795
30	27.166607780088263	22.695205768919998	24.269031888458564	25.869154562533176
31	28.708729063554433	22.89678569959684	23.716739473362917	24.67774576348581
32	29.51308949863935	23.32682504403539	23.45162033086377	23.70846512646149
33	29.461306012551745	22.46198577200952	24.32058868011051	23.756119535328224
34	28.364995529261876	23.264807923423916	25.733445557873956	22.636750989440248
35	27.428974204440166	24.80114849878087	25.467209135656216	22.302668161122746
36	28.133896744570748	24.211856313294298	24.112062184226797	23.542184757908156
37	27.794308479958758	25.10998728244216	22.819871516070855	24.275832721528232
38	28.179397556298753	25.202559574488063	22.884333698095475	23.733709171117702
39	27.70568714807713	23.39161107525381	22.796586759037094	26.10611501763197
40	27.42931424609365	24.28350794742114	22.806771816181904	25.480405990303307
41	25.744618355059835	24.686505884177926	23.67611259200151	25.89276316876073
42	25.051581080341478	22.906209711136235	24.8149120895171	27.22729711900519
43	24.94276775122682	23.032235624901023	26.060662783283036	25.96433384058912
44	26.030059034469538	22.503244159298827	25.382975960350496	26.083720845881142
45	26.10605024779321	23.056297619999892	24.881187827026963	25.95646430517994
46	26.399749081644647	24.295830409244985	23.45128028921028	25.853140219900077
47	25.953420122758274	24.342157036417166	24.95669326655995	24.747729574264614
48	26.418807606699403	24.212584973980338	24.55377629210161	24.81483112721865
49	26.853656111665792	23.642157004032246	24.14324886158912	25.360938022712837
50	24.970489242215557	24.42020469212143	24.48520122531581	26.124104840347208
51	24.935529721745535	24.52535852534622	24.134262046461348	26.404849706446896
52	25.464780266702764	24.67112304747273	24.265453354867144	25.59864333095736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	32.5
2	65.0
3	69.0
4	73.0
5	151.0
6	229.0
7	2895.5
8	5562.0
9	11232.0
10	16902.0
11	18606.5
12	20311.0
13	20097.5
14	18610.5
15	17337.0
16	17467.0
17	17597.0
18	18653.0
19	19709.0
20	22097.0
21	24485.0
22	29310.0
23	34135.0
24	41013.0
25	47891.0
26	51702.5
27	55514.0
28	62733.5
29	69953.0
30	80983.5
31	92014.0
32	100429.5
33	108845.0
34	122787.5
35	136730.0
36	147053.5
37	157377.0
38	162417.5
39	180351.0
40	193244.0
41	208785.5
42	224327.0
43	244323.5
44	264320.0
45	275578.0
46	286836.0
47	303314.0
48	319792.0
49	335599.5
50	351407.0
51	365401.5
52	379396.0
53	423475.0
54	467554.0
55	485288.0
56	503022.0
57	489811.0
58	476600.0
59	472992.0
60	469384.0
61	423658.0
62	377932.0
63	332485.0
64	235391.5
65	183745.0
66	156130.0
67	128515.0
68	113581.5
69	98648.0
70	88659.5
71	78671.0
72	61264.0
73	43857.0
74	30340.0
75	16823.0
76	12540.0
77	8257.0
78	5748.5
79	3240.0
80	2013.5
81	787.0
82	489.0
83	191.0
84	111.0
85	31.0
86	23.0
87	15.0
88	10.5
89	5.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	6175714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.157737805937902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.96464148212013	5.5443772528570685
2	9.648860903000482	1.5742575474528633
3	4.2119020847456206	1.0307877861491404
4	1.9804850232093625	0.6462511019171527
5	1.1688272644937192	0.4767493182085696
6	0.6913699237044332	0.33840087386952344
7	0.49297943089517293	0.2815117858674312
8	0.32297222125132147	0.21077781596557163
9	0.2680207598377291	0.19677987767740007
>10	6.119151662732506	14.568483038858734
>50	4.491534701330957	25.89253632728849
>100	2.5159381430763923	35.78165729990826
>500	0.08621785249489582	4.708912754832936
>1k	0.03233169468558593	4.733783356175631
>5k	0.0026943078904654943	1.3528355992085044
>10k+	0.002072544531127303	2.6618982637628714
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACAATAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	23976	0.38823041351979704	No Hit
ACAATAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	23187	0.37545456282463857	No Hit
ACAATAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	18781	0.3041105854319031	No Hit
ACAATAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16208	0.2624473866503533	No Hit
ACAATAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	14793	0.23953505618945437	No Hit
ACAATAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	12844	0.20797595225426568	No Hit
ACAATAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	12764	0.20668055547909117	No Hit
ACAATAGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	12609	0.2041707242271906	No Hit
ACAATAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11274	0.18255379054146614	No Hit
ACAATAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	11005	0.17819801888494188	No Hit
ACAATAGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	6979	0.11300717617428527	No Hit
ACAATAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	6550	0.10606061096741203	No Hit
ACAATAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	6451	0.10445755745813359	No Hit
ACAATAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	6343	0.10270877181164802	No Hit
ACAATAGGGAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGT	6322	0.10236873015816471	No Hit
ACAATAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	6235	0.10095998616516243	No Hit
ACAATAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6214	0.10061994451167915	No Hit
ACAATAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	6198	0.10036086515664423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.04572750616365978	0.0	0.0	0.0	0.0
7	0.04723340491480014	0.0	0.0	0.0	0.0
8	0.0597663687146134	0.0	0.0	0.0	0.0
9	0.0785658144143333	0.0	0.0	0.0	0.0
10	0.10861901959838166	0.0	0.0	0.0	0.0
11	0.13123988578486634	0.0	0.0	0.0	0.0
12	0.14301180397926458	0.0	0.0	0.0	0.0
13	0.15156142269541628	0.0	0.0	0.0	9.715475813808736E-4
14	0.16080731717822425	0.0	0.0	0.0	9.715475813808736E-4
15	0.17219061634007016	0.0	0.0	0.0	9.715475813808736E-4
16	0.1843997309460898	0.0	0.0	0.0	9.715475813808736E-4
17	0.1969003098265237	0.0	0.0	0.0	9.715475813808736E-4
18	0.20847791850464578	0.0	0.0	0.0	9.715475813808736E-4
19	0.22361786831449773	0.0	0.0	0.0	9.715475813808736E-4
20	0.23377054053992785	0.0	0.0	0.0	9.715475813808736E-4
21	0.24351840127311594	0.0	0.0	0.0	9.715475813808736E-4
22	0.25308814494971754	0.0	0.0	0.0	9.715475813808736E-4
23	0.26367801358676907	0.0	0.0	0.0	9.715475813808736E-4
24	0.27439742190133803	0.0	0.0	0.0	9.715475813808736E-4
25	0.28516540759497605	0.0	0.0	0.0	9.715475813808736E-4
26	0.29750406187851314	0.0	0.0	0.0	9.877400410705547E-4
27	0.3082558551124615	0.0	0.0	0.0	9.877400410705547E-4
28	0.3225861819378294	0.0	0.0	0.0	9.877400410705547E-4
29	0.33345132238960545	0.0	0.0	0.0	9.877400410705547E-4
30	0.3443002703816919	0.0	0.0	0.0	9.877400410705547E-4
31	0.35489013901874344	0.0	0.0	0.0	9.877400410705547E-4
32	0.3672773706813496	0.0	0.0	0.0	9.877400410705547E-4
33	0.3774300429067797	0.0	0.0	0.0	9.877400410705547E-4
34	0.3899144293275239	0.0	0.0	0.0	9.877400410705547E-4
35	0.40288458953895856	0.0	0.0	0.0	9.877400410705547E-4
36	0.4141545414829767	0.0	0.0	0.0	9.877400410705547E-4
37	0.4254568783463742	0.0	0.0	0.0	9.877400410705547E-4
38	0.4361277092818741	0.0	0.0	0.0	9.877400410705547E-4
39	0.4470738120320986	0.0	0.0	0.0	9.877400410705547E-4
40	0.4589752699040143	0.0	0.0	0.0	9.877400410705547E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGAT	70845	0.0	44.668926	5
ATAGGGA	215835	0.0	44.663704	4
CAATAGG	630280	0.0	44.502014	2
AATAGGG	617240	0.0	44.500923	3
ACAATAG	636315	0.0	44.497066	1
ATAGGGG	325780	0.0	44.287247	4
ATAGGGT	28775	0.0	44.28149	4
TAGGGGG	112500	0.0	44.27449	5
TAGGGAG	67095	0.0	44.272305	5
TAGGGGA	95120	0.0	44.266296	5
AGGGATA	10175	0.0	44.10123	6
ATAGGGC	48310	0.0	44.000412	4
TAGGGGT	60150	0.0	43.847218	5
TAGGGAC	40305	0.0	43.671753	5
AGGGATT	25680	0.0	43.536995	6
TAGGGGC	59740	0.0	43.505188	5
AGGGATC	18645	0.0	43.40949	6
TAGGGAA	40485	0.0	43.096947	5
TAGGGTA	7560	0.0	42.957672	5
TAGGGTG	11930	0.0	42.780388	5
>>END_MODULE