FastQCFastQC Report
Fri 17 Jun 2016
SRR1527765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381547
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC13630.3572299087661546No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT11800.3092672724461207No Hit
TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA11680.3061221815398889No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC10480.27467127247757156No Hit
TTGGACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC9130.23928899978246454No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6290.16485518166831348No Hit
TTGGACGGGCTGGGGCTACAGAGCTGACTGACGTCACAGGATTCAAACCA6180.16197218167093438No Hit
TTGGACGGGGATATTACCTGTGAGAAACTCACTTTAAATACAATGGTGTG5770.15122645440797594No Hit
TTGGACGGGAGCGTAGAGCCGGGCGCGGTGGCGCGCGTCTTTAATCCCAG5510.14441209077780717No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC5220.1368114544210805No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG5110.1339284544237014No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCA4820.1263278180669747No Hit
TTGGACGGGGGGAGCATGTAGAAGGGGAGGGAGGGAGAGAGGGAGGGAAG4760.12475527261385884No Hit
TTGGACGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC4740.12423109079615355No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4710.12344481806959562No Hit
TTGGACGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA4550.1192513635279533No Hit
TTGGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCT4280.11217490898893191No Hit
TTGGACGGGACAAATGTGAAGGCCGAGGTGGTAAGAAAGGAGCAGTAGAG4260.11165072717122661No Hit
TTGGACGGGAGAGGCTCCTTCCTGCAGTGTCAGCATCAGCCATGTTGGGT4060.10640890899417373No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA3920.10273963627023669No Hit
TTGGACGGGATTTCTGTCTCATGCTTTGCAGTCTTAGCTCCCTTTTAAAG3850.10090499990826818No Hit
TTGGACGGGAGCGGTGAAATTGTGAACTCTTGGAAATGCTGTCAATCAAG3850.10090499990826818No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT3820.10011872718171025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG650.044.00000427
GTTGAAC207.849861E-444.033
CGGAATA254.437231E-544.06
TAATAGC207.849861E-444.017
TCGTTTA207.849861E-444.031
AGTCCGC207.849861E-444.035
GGTACGA408.287316E-944.015
GTACCGC207.849861E-444.018
TCCATAA254.437231E-544.042
CGAACCC302.5232548E-644.022
TCCGCGT351.4426769E-744.029
CAACGTC254.437231E-544.036
CCGATTA207.849861E-444.037
CGCGGAA302.5232548E-644.028
TCCGATT254.437231E-544.036
ACGCACC254.437231E-544.042
GCGAACC207.849861E-444.09
CGGCCAA207.849861E-444.018
GCAACCG207.849861E-444.023
AGCTTAT207.849861E-444.040
Produced by FastQC (version 0.11.5)