##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527765_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	381547
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.538615164055805	34.0	31.0	34.0	31.0	34.0
2	32.98139678728964	34.0	31.0	34.0	31.0	34.0
3	32.993712439096626	34.0	31.0	34.0	31.0	34.0
4	36.440753039599315	37.0	37.0	37.0	35.0	37.0
5	36.035633879967605	37.0	35.0	37.0	35.0	37.0
6	36.3690292414827	37.0	37.0	37.0	35.0	37.0
7	36.49952168409134	37.0	37.0	37.0	35.0	37.0
8	36.66407546121448	37.0	37.0	37.0	35.0	37.0
9	38.72229109388883	39.0	39.0	39.0	39.0	39.0
10	38.15956094530949	39.0	39.0	39.0	37.0	39.0
11	37.85753000285679	39.0	38.0	39.0	35.0	39.0
12	37.482320657743344	39.0	37.0	39.0	35.0	39.0
13	37.335313866967894	39.0	37.0	39.0	35.0	39.0
14	38.14134038532605	40.0	38.0	40.0	34.0	40.0
15	38.20147452345321	40.0	38.0	40.0	35.0	40.0
16	38.20803990071996	40.0	38.0	40.0	35.0	40.0
17	38.126736679884786	40.0	38.0	40.0	34.0	40.0
18	38.05451752995044	40.0	38.0	40.0	34.0	40.0
19	38.01919291725528	40.0	38.0	40.0	34.0	40.0
20	37.864525209214065	40.0	38.0	40.0	34.0	40.0
21	37.82841956561053	40.0	37.0	40.0	34.0	40.0
22	37.84208760650719	40.0	37.0	40.0	34.0	40.0
23	37.740419922054166	40.0	37.0	40.0	34.0	40.0
24	37.67140614393509	40.0	37.0	40.0	34.0	40.0
25	37.67082430211743	40.0	37.0	40.0	33.0	40.0
26	37.54407451768721	39.0	37.0	40.0	33.0	40.0
27	37.456232128676156	39.0	37.0	40.0	33.0	40.0
28	37.242772187961116	39.0	36.0	40.0	33.0	40.0
29	37.44081069959926	40.0	37.0	40.0	33.0	40.0
30	37.680511182108624	40.0	37.0	40.0	33.0	40.0
31	37.70502978663179	40.0	37.0	40.0	34.0	40.0
32	37.66549599394046	40.0	37.0	40.0	33.0	40.0
33	37.577386272202375	40.0	37.0	40.0	33.0	40.0
34	37.290108427008995	40.0	36.0	40.0	33.0	40.0
35	37.31038377971783	40.0	36.0	40.0	33.0	40.0
36	37.22332504252425	40.0	35.0	40.0	33.0	40.0
37	37.15320261986073	40.0	35.0	40.0	33.0	40.0
38	37.053458682678674	40.0	35.0	40.0	33.0	40.0
39	36.97297842729729	39.0	35.0	40.0	33.0	40.0
40	36.82012700925443	39.0	35.0	40.0	32.0	40.0
41	36.71249151480683	39.0	35.0	40.0	32.0	40.0
42	36.59071359491753	39.0	35.0	40.0	32.0	40.0
43	36.468209683210716	39.0	35.0	40.0	32.0	40.0
44	36.20221886163435	38.0	35.0	40.0	31.0	40.0
45	36.17516583802258	38.0	35.0	40.0	31.0	40.0
46	35.95813359821988	38.0	35.0	40.0	31.0	40.0
47	35.887819849192894	37.0	35.0	40.0	31.0	40.0
48	35.824194660159826	37.0	35.0	40.0	31.0	40.0
49	35.68416472937803	37.0	35.0	40.0	30.0	40.0
50	35.16018210076347	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	4.0
15	5.0
16	13.0
17	17.0
18	35.0
19	74.0
20	147.0
21	252.0
22	380.0
23	516.0
24	807.0
25	1221.0
26	1635.0
27	2105.0
28	2823.0
29	3682.0
30	4980.0
31	6323.0
32	8392.0
33	11600.0
34	21546.0
35	26480.0
36	38154.0
37	61053.0
38	132896.0
39	56402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.92333054643333	0.9264913627940987	0.12161018170762711	0.028567909064938266
8	99.38644518237597	0.4728119995701709	0.12501736352271148	0.015725454531158676
9	96.953193184588	1.7394973620550025	0.5393830904187427	0.7679263629382488
10	47.373718047842075	39.046041509958144	5.192545086188596	8.387695356011186
11	32.614592697623095	24.298710250637537	23.581891614925553	19.504805436813815
12	27.30489297517737	19.67070898211754	27.341061520599037	25.68333652210606
13	24.025873614521934	19.345192073322554	30.49584979045832	26.133084521697196
14	24.961538159125872	19.964512890941354	30.46413679048715	24.609812159445628
15	25.04147588632593	22.495000615913636	27.619402065800543	24.84412143195989
16	28.337006974239088	23.48334543319696	24.636021250330888	23.54362634223307
17	28.661737610307515	23.3093170697188	23.13659916078491	24.892346159188776
18	27.36045624785413	22.811344342898778	24.282722705197525	25.54547670404957
19	28.069412156300533	23.734428523877792	23.43512070596807	24.7610386138536
20	28.568695337664824	22.81763452471124	24.19701897800271	24.416651159621225
21	29.256683973403014	21.08835870810149	25.69146134028049	23.963495978215004
22	27.763027883851795	21.637963343965488	25.762487976579557	24.83652079560316
23	26.743494248415008	23.460019342309074	25.334231431514336	24.462254977761585
24	26.38128461238065	23.57481516038653	25.045669340867573	24.998230886365246
25	26.5597685213093	25.038068704510845	23.30800661517454	25.094156159005315
26	25.400278340545203	26.449428248682338	21.84501516195908	26.305278248813384
27	26.156934794402787	24.004906341813722	23.846865523775577	25.991293340007914
28	26.26439206703237	25.27709561338446	23.06242743357961	25.39608488600356
29	26.78909806655537	24.121274705344295	24.49632679591243	24.59330043218791
30	27.51718661134801	22.320448070617775	24.725656341158494	25.43670897687572
31	27.710609702081264	24.625275523067934	24.24734043250242	23.416774342348386
32	28.775485064749557	24.497637250456695	23.35387252422375	23.373005160569996
33	29.29888060972829	23.304599433359456	23.097547615365865	24.29897234154639
34	27.01816552089258	26.02536515815876	24.23947770523684	22.716991615711827
35	27.249329702500606	25.511929067716427	24.925631704613064	22.3131095251699
36	26.637609521238538	25.67154243120769	24.047102978138998	23.643745069414777
37	26.654121248496253	26.881878248289205	23.109603797173087	23.35439670604146
38	26.902059248270856	26.42557797597675	23.623826160341977	23.048536615410423
39	26.06913433993715	25.02679879543018	22.96335707003331	25.940709794599353
40	26.2376587943294	25.650050976681772	22.72957197933675	25.382718249652076
41	24.871116795571712	26.225340521613326	23.98603579637633	24.91750688643863
42	25.22467743161393	25.948572521864932	23.36252152421589	25.46422852230525
43	25.143167158960754	25.12455870443222	25.17619061347619	24.556083523130834
44	25.38429079510519	25.069257522664312	24.301855341543767	25.24459634068673
45	25.195585340731284	25.07712024992989	24.040812796326534	25.68648161301229
46	25.99365215818759	24.369212705118898	24.084319887196074	25.552815249497442
47	24.618461159437764	24.91776897734748	25.158106340765357	25.305663522449397
48	26.66486697575921	24.27328743247883	24.54822079586525	24.513624795896703
49	24.236332614330607	26.01881288543744	25.129014249882715	24.615840250349237
50	24.31600825062181	25.591866794916484	25.298849158819227	24.79327579564248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.0
6	6.0
7	4.5
8	3.0
9	3.5
10	4.0
11	114.0
12	224.0
13	557.0
14	890.0
15	1118.5
16	1347.0
17	1210.5
18	1074.0
19	1081.0
20	1088.0
21	1132.5
22	1177.0
23	1245.0
24	1313.0
25	1573.5
26	1834.0
27	1809.5
28	1785.0
29	2575.5
30	3366.0
31	3476.5
32	3587.0
33	4335.0
34	5083.0
35	6121.5
36	7160.0
37	7581.5
38	8003.0
39	9098.5
40	10194.0
41	11693.0
42	13192.0
43	14516.5
44	15841.0
45	15643.0
46	15445.0
47	18305.0
48	21165.0
49	21000.5
50	20836.0
51	22695.5
52	24555.0
53	24892.0
54	25229.0
55	24731.0
56	24233.0
57	28283.5
58	32334.0
59	30232.0
60	28130.0
61	29655.5
62	31181.0
63	27557.5
64	23934.0
65	21273.5
66	18613.0
67	15360.0
68	12107.0
69	10341.5
70	8576.0
71	8098.0
72	7620.0
73	5710.5
74	3801.0
75	4190.0
76	4579.0
77	2898.0
78	1217.0
79	910.0
80	603.0
81	354.5
82	106.0
83	100.5
84	95.0
85	53.5
86	12.0
87	7.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	381547.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.657001627584544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.68316777940618	6.632734630333878
2	8.660370189437117	1.672664180297578
3	3.321934538348803	0.9623978173069111
4	1.5822613038050264	0.6111959994443672
5	0.7599196656353472	0.3669272723937025
6	0.537371763556424	0.31136399971694184
7	0.3826738316235141	0.25868372703756026
8	0.3962438256527167	0.3061221815398889
9	0.3229658578950225	0.2806993633811824
>10	8.758074146447376	25.171735068025697
>50	4.706073929327472	31.68128697119883
>100	1.8590891820007598	29.364403336941454
>500	0.018997991640883678	1.1324948171522775
>1k	0.010855995223362102	1.247290635229736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1363	0.3572299087661546	No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1180	0.3092672724461207	No Hit
TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	1168	0.3061221815398889	No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1048	0.27467127247757156	No Hit
TTGGACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	913	0.23928899978246454	No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	629	0.16485518166831348	No Hit
TTGGACGGGCTGGGGCTACAGAGCTGACTGACGTCACAGGATTCAAACCA	618	0.16197218167093438	No Hit
TTGGACGGGGATATTACCTGTGAGAAACTCACTTTAAATACAATGGTGTG	577	0.15122645440797594	No Hit
TTGGACGGGAGCGTAGAGCCGGGCGCGGTGGCGCGCGTCTTTAATCCCAG	551	0.14441209077780717	No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	522	0.1368114544210805	No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	511	0.1339284544237014	No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCA	482	0.1263278180669747	No Hit
TTGGACGGGGGGAGCATGTAGAAGGGGAGGGAGGGAGAGAGGGAGGGAAG	476	0.12475527261385884	No Hit
TTGGACGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	474	0.12423109079615355	No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	471	0.12344481806959562	No Hit
TTGGACGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAA	455	0.1192513635279533	No Hit
TTGGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCT	428	0.11217490898893191	No Hit
TTGGACGGGACAAATGTGAAGGCCGAGGTGGTAAGAAAGGAGCAGTAGAG	426	0.11165072717122661	No Hit
TTGGACGGGAGAGGCTCCTTCCTGCAGTGTCAGCATCAGCCATGTTGGGT	406	0.10640890899417373	No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	392	0.10273963627023669	No Hit
TTGGACGGGATTTCTGTCTCATGCTTTGCAGTCTTAGCTCCCTTTTAAAG	385	0.10090499990826818	No Hit
TTGGACGGGAGCGGTGAAATTGTGAACTCTTGGAAATGCTGTCAATCAAG	385	0.10090499990826818	No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	382	0.10011872718171025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.05451490904135008	0.0	0.0	0.0	0.0
8	0.05661163631217124	0.0	0.0	0.0	0.0
9	0.057659999947581815	0.0	0.0	0.0	0.0
10	0.06709527266627703	0.0	0.0	0.0	0.0
11	0.07495799993185637	0.0	0.0	0.0	0.0
12	0.08622790901252009	0.0	0.0	0.0	0.0
13	0.09592527264006794	0.0	0.0	0.0	0.0
14	0.10405009081449992	0.0	0.0	0.0	0.0
15	0.11348536353319512	0.0	0.0	0.0	0.0
16	0.12554154534041678	0.0	0.0	0.0	0.0
17	0.13759772714763843	0.0	0.0	0.0	0.0
18	0.146770908957481	0.0	0.0	0.0	0.0
19	0.16118590894437645	0.0	0.0	0.0	0.0
20	0.17376627256930338	0.0	0.0	0.0	0.0
21	0.18398781801455652	0.0	0.0	0.0	0.0
22	0.19289890891554645	0.0	0.0	0.0	0.0
23	0.20574136344932603	0.0	0.0	0.0	0.0
24	0.2167491816211371	0.0	0.0	0.0	0.0
25	0.2277569997929482	0.0	0.0	0.0	0.0
26	0.24086154523558043	0.0	0.0	0.0	0.0
27	0.257373272493297	0.0	0.0	0.0	0.0
28	0.27205036338904515	0.0	0.0	0.0	0.0
29	0.2848928179228247	0.0	0.0	0.0	0.0
30	0.297211090638899	0.0	0.0	0.0	0.0
31	0.30638427244874156	0.0	0.0	0.0	0.0
32	0.3187025451648159	0.0	0.0	0.0	0.0
33	0.3281378178835111	0.0	0.0	0.0	0.0
34	0.3375730906022063	0.0	0.0	0.0	0.0
35	0.35460899967762816	0.0	0.0	0.0	0.0
36	0.3669272723937025	0.0	0.0	0.0	0.0
37	0.37793509056551355	0.0	0.0	0.0	0.0
38	0.3884187269196193	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	65	0.0	44.000004	27
GTTGAAC	20	7.849861E-4	44.0	33
CGGAATA	25	4.437231E-5	44.0	6
TAATAGC	20	7.849861E-4	44.0	17
TCGTTTA	20	7.849861E-4	44.0	31
AGTCCGC	20	7.849861E-4	44.0	35
GGTACGA	40	8.287316E-9	44.0	15
GTACCGC	20	7.849861E-4	44.0	18
TCCATAA	25	4.437231E-5	44.0	42
CGAACCC	30	2.5232548E-6	44.0	22
TCCGCGT	35	1.4426769E-7	44.0	29
CAACGTC	25	4.437231E-5	44.0	36
CCGATTA	20	7.849861E-4	44.0	37
CGCGGAA	30	2.5232548E-6	44.0	28
TCCGATT	25	4.437231E-5	44.0	36
ACGCACC	25	4.437231E-5	44.0	42
GCGAACC	20	7.849861E-4	44.0	9
CGGCCAA	20	7.849861E-4	44.0	18
GCAACCG	20	7.849861E-4	44.0	23
AGCTTAT	20	7.849861E-4	44.0	40
>>END_MODULE