FastQCFastQC Report
Fri 17 Jun 2016
SRR1527764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581364
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT20100.3457386422275889No Hit
TTGGACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG17330.29809207312458286No Hit
TTGGACGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA17160.2951679154540013No Hit
TTGGACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC16710.28742749809069706No Hit
TTGGACGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG13420.23083644670120612No Hit
TTGGACGGGCTGGGGCTACAGAGCTGACTGACGTCACAGGATTCAAACCAAT9600.16512890375049022No Hit
TTGGACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT9260.15928058840932702No Hit
TTGGACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT8850.15222820814498317No Hit
TTGGACGGGAGCGTAGAGCCGGGCGCGGTGGCGCGCGTCTTTAATCCCAGCA8520.1465519020785601No Hit
TTGGACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC7850.13502728067097378No Hit
TTGGACGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT7320.1259107891097488No Hit
TTGGACGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC6810.11713831609800401No Hit
TTGGACGGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGA6740.11593425117482335No Hit
TTGGACGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6640.1142141584274224No Hit
TTGGACGGGGATATTACCTGTGAGAAACTCACTTTAAATACAATGGTGTGTG6470.11129000075684081No Hit
TTGGACGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA6420.11042995438314034No Hit
TTGGACGGGGGGAGCATGTAGAAGGGGAGGGAGGGAGAGAGGGAGGGAAGAA6070.10440962976723704No Hit
TTGGACGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA5980.10286154629457621No Hit
TTGGACGGGACCCATGAGGTTGCTGCCGAGCCTGACAGCCTGAATTCAGTCT5960.10251752774509601No Hit
TTGGACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT5880.10114145354717527No Hit
TTGGACGGGAGAGGCTCCTTCCTGCAGTGTCAGCATCAGCCATGTTGGGTCT5880.10114145354717527No Hit
TTGGACGGGACAAATGTGAAGGCCGAGGTGGTAAGAAAGGAGCAGTAGAGTG5870.10096944427243518No Hit
TTGGACGGGACCTTGGGAGATTGTAACAGTGACCAAATTATCTGTGCATTTA5820.10010939789873469No Hit
TTGGACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5820.10010939789873469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG351.0183976E-746.00000435
CTAGAAT351.0183976E-746.00000439
GGTACGC351.0183976E-746.0000049
TTTACGC351.0183976E-746.00000436
CGATTGA351.0183976E-746.00000420
TTACGCT351.0183976E-746.00000437
CGGTCGT351.0183976E-746.00000428
CACGTAT351.0183976E-746.00000432
CGATACA700.046.00000446
TACACGT351.0183976E-746.00000430
ACGGTAT351.0183976E-746.0000045
ACGCGGT351.0183976E-746.00000443
TCGCCTT206.3088676E-446.030
CGGAATT301.8598039E-646.014
CGGAATA206.3088676E-446.06
CTAGACG253.4150573E-546.026
AGCGTAC301.8598039E-646.015
CAGCGAA206.3088676E-446.030
TGATACG253.4150573E-546.026
GATACGC253.4150573E-546.027
Produced by FastQC (version 0.11.5)